AI Summary & Key Takeaways
AI InsightIt was found that the amperometric responses of the sensor decreased with increasing the volume of coating solution dropped on the adsorbed enzyme.
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Abstract
In the present study, the effect of the film thickness on the responses of a glucose biosensor was investigated. The modified platinum electrodes used in the study were constructed by immobilization of glucose oxidase under a film layer of the sol-gel coating solution. The sol-gel coating solutions were prepared by using (3-Glycidoxylpropyl) trimethoxysilane (GLYMO), tetraethoxysilane (TEOS), and methyltriethoxysilane (MTEOS). Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide released by the enzymatic reaction between glucose and glucose oxidase. It was found that the amperometric responses of the sensor decreased with increasing the volume of coating solution dropped on the adsorbed enzyme. It was determined that the film thickness has a significantly influence on the sensor responses. It was observed that the amperometric responses decreased by 85% when the volume of the coating solution dropped on the electrode increased from 4 µL to 12 µL.