Journals / Journal of advances in vetbio science and techniques / 2021 / Cilt: 6 - Sayı: 1

Western Blot

Pages
45–56
Publication Date
2022-10-19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this composition, , information has been provided about principle, protocol and use of Western Blot technique in virology.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The Western Blot technique is an old and reliable method used in molecular biology to uniquely detect a single protein from any sample.
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Key Findings & Data:It is a frequently used method for investigating the responses of proteins to stimuli, evaluating protein expression as either absent or increased-decreased, determining cell specific protein isoforms and determining proteins responsible for the occurrence of genetic diseases.
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Conclusion & Impact:In this composition, , information has been provided about principle, protocol and use of Western Blot technique in virology.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Metot · Violoji · Western Blot

Abstract

The Western Blot technique is an old and reliable method used in molecular biology to uniquely detect a single protein from any sample. It is a frequently used method for investigating the responses of proteins to stimuli, evaluating protein expression as either absent or increased-decreased, determining cell specific protein isoforms and determining proteins responsible for the occurrence of genetic diseases. In this composition, , information has been provided about principle, protocol and use of Western Blot technique in virology.

Özet

Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının belirlenmesinde, genetik hastalıkların ortaya çıkışından sorumlu proteinlerin belirlenmesinde sıklıkla kullanılan bir metottur. Yapılan bu derlemede, Western Blot tekniğinin prensibi, protokolü ve virolojide kullanımı hakkında bilgi verilmiştir.

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Cite This Article

BULUT, O., ASLIM, H. P. (2022). Western Blot. Journal of advances in vetbio science and techniques, Cilt: 6 - Sayı: 1, 45–56. https://ajindex.com/articles/559445

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References (8)

  1. Anonim, (2009). Polyacrylamide gel electrophoresis. https://en.wikipedia.org/wiki/Polyacrylamide gel electrophoresis Erişim tarihi: 27.07.2020 Anonim, (2020a, 27 Temmuz). Western blot. https://en.wikipedia.org/wiki/Western_blot#:~:text=SDS%20is%20generally%20used%20as,%2C%20negatively%2C%20or%20neutrally%20charged.&text=The%20gel%20electrophoresis%20step%20is,the%20cross%2Dreactivity%20of%20antibodies Anonim, (2020b, 27 Temmuz). Choosing a primary antibody or secondary antibody. https://www.abcam.com/protocols/choosing-an-antibody CUSABIO, (2018, 27 Temmuz 2020 ). Laboratory technical manual western blotting. Erişim adresi: https://www.cusabio.com/protocols/Western_Blotting(WB)_Protocol.pdf
  2. Flores, M.K., Salazar-Anzures, Á.T., Fernández-Torres, J., Pineda, C., Aguilar-González, C.A., López-Reyes, A. (2017). Western blot: a tool in the biomedical field. Investigacion en Discapacidad. 3, 128-137.
  3. Hassan, A.O., Vemula, S.V., Sharmal, A., Bangaril, D.S., Mishral, K.K., Mitta, S.K. (2017). 155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication. Journal of General Virology, 98, 749-753.
  4. He, Q., Chong, K.H., Chng, H.K., Leung, B., Ling, A.E., Wei, T., Chan, S.W., Ooi, E.E.İ., Kwang, J. (2004). Development of a Western blot assay for detection of antibodies against coronavirus causing severe acute respiratory syndrome. American society for mıcrobıyolog. 11, 417-422.
  5. Hemmatzadeh, F., Kazemimanesh, M. (2017). Detection of specific antigens of newcastle disease virus using an absorbed Western blotting method. Iranian Journal of Veterinary Research, 18, 92-96.
  6. Jayme, S.I., Field, H.E., Jong, C.D., Olival, K.J., Marsh, G., Tagtag, A.M., Hughes, T., Bucad, A.C., Barr, J., Azul, R.R., Retes, L.M., Foord, A., Yu, M., Cruz, M.S., Santos, I.J., Lim, T.M.S., Benigno, C.C., Epstein, J.H., Wang, L.F., Daszak, P., Newman, S.H. (2015). Molecular evidence of Ebola Reston virus infection in Philippine bats. Virology Journal,, 12,107. Jensen, E.C. (2012). The Basics of Western Blotting. The Anatomical Record, 295, 369–371.
  7. Kennedy, M. (2005). Methodology in diagnostic virology. Veterinary Clinics: Exotic Animal Practice, 8, 7–26.
  8. Goldberg, T.L., Chapman, C.A., Cameron, K., Saj, T., Karesh, W.B., Wolfe, N.D., Wong, S.W., Dubois, M.E., Slifka, M. (2008). Serologic evidence for novel poxvirus in endangered red colobus monkeys, Western Uganda. Emerging Infectious Disease,. 5, 801. Kurien, B.T., Scofield, R. (2006). Western blotting