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Journals / Journal of advances in vetbio science and techniques / 2026 / Issue: 3

Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index

Süleyman Nogay(MALATYA TURGUT OZAL UNIVERSITY, SCHOOL OF MEDICINE)0000-0003-1184-9467
Turgut Dolanbay(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-4092-1192
Tahir Gezer(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0001-7838-2553
Merve Sena Demir(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0002-2630-8818
Bilgehan Demir(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-3458-2398
Mehmet Sezer(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-5945-7895
Merve Ayyıldız Akın(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0002-7362-7934
Pages
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Publication Date
2026-10-22
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In conclusion, it has been determined that cyclophosphamide administration increases oxidative stress and impairs antioxidant defense, as evidenced by decreases in TAS and CAT levels and increases in TOS, OSI, and NO levels.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to evaluate cyclophosphamide-induced toxicity in rats by assessing levels of catalase (CAT), nitric oxide (NO), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI).
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Key Findings & Data:A total of 18 female Wistar albino rats were divided into three groups, with six rats in each group.
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Conclusion & Impact:In conclusion, it has been determined that cyclophosphamide administration increases oxidative stress and impairs antioxidant defense, as evidenced by decreases in TAS and CAT levels and increases in TOS, OSI, and NO levels.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cyclophosphamide-induced toxicity · CAT · TAS

Abstract

This study aimed to evaluate cyclophosphamide-induced toxicity in rats by assessing levels of catalase (CAT), nitric oxide (NO), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI). A total of 18 female Wistar albino rats were divided into three groups, with six rats in each group. The groups established were: a healthy control group (C, n=6), an ovariectomized group (O, n=6), and a group that received a single intraperitoneal (IP) dose of 200 mg/kg cyclophosphamide following ovariectomy (O+CPA, n=6). At the end of the study (24 hours after cyclophosphamide administration), the rats were euthanized via cervical dislocation under anesthesia in accordance with ethical guidelines, and blood samples were collected. Biochemical measurements (Catalase, Nitric Oxide, Total Antioxidant, and Total Oxidant) were performed on the collected blood samples. Analysis of the TAS, TOS, and OSI results revealed that TAS levels decreased, while TOS and OSI levels increased in the O+CPA group. In the O+CPA group, CAT levels decreased, whereas NO levels increased significantly. In conclusion, it has been determined that cyclophosphamide administration increases oxidative stress and impairs antioxidant defense, as evidenced by decreases in TAS and CAT levels and increases in TOS, OSI, and NO levels.

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Cite This Article

Nogay, S., Dolanbay, T., Gezer, T., Demir, M. S., Demir, B., Sezer, M., Akın, M. A. (2026). Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index. Journal of advances in vetbio science and techniques, Issue: 3. https://doi.org/10.31797/vetbio.2054243

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