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Journals / Ondokuz Mayıs Üniv. Diş Hekimliği Derg. / 2004 / Cilt: 5 - Sayı: 2

Evaluation of the systemic doxycycline therapy on alveolar bone, periodontal ligament by ultrastructural analysis and serum interleukin-6 level determination in experimental periodontitis

Deneysel periodontitis modelinde sistemik doksisiklin uygulamasının ultrastrüktürel açıdan alveol kemiği, periodontal ligamente ve serum interlökin-6 düzeyine etkisinin değerlendirilmesi

Gonca KELEŞ
Burcu ÖZKAN
Musa ALDIKAÇTI
Neşe LORTLAR
Gökhan AÇIKGÖZ
Erhan FIRATLI
Pages
17–21
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Ultrastructural and serum IL-6 level analysis show that oral administration of doxycycline did not induce healing of alveolar bone and periodontal ligament in experimental periodontitis in rats.

📌 3 Key Takeaways

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Objective & Hypothesis:Aitn: The aim of this study is ultrastructural evaluation in alveolar bone and periodontal ligament and serum interleukin-6 (IL-6) determination of the systemic doxycycline therapy in experimental periodontitis in rats.
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Key Findings & Data:Material and Methods: 40 Wistar rats weighting 250-300 gm were used. 30 rats were used as test and 10 rats were used as control group.
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Conclusion & Impact:Conclusion: Ultrastructural and serum IL-6 level analysis show that oral administration of doxycycline did not induce healing of alveolar bone and periodontal ligament in experimental periodontitis in rats.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Doksisiklin · Periodontal ligament · Periodontit

Abstract

Aitn: The aim of this study is ultrastructural evaluation in alveolar bone and periodontal ligament and serum interleukin-6 (IL-6) determination of the systemic doxycycline therapy in experimental periodontitis in rats. Material and Methods: 40 Wistar rats weighting 250-300 gm were used. 30 rats were used as test and 10 rats were used as control group. Ligature-induced periodontitis was obtained in 30 rats' mandibular molar teeth. 10 rats in ligature-induced periodontitis group (Group I), and control rats were decapitated, after fovty days, and blood was obtained by cardiac puncture. After forty days; in 10 rats doxycycline at dose of 21 rug/day was administrated orally (Group 2), in the remaining 10 rats no medication was performed for 15 days (Group 3). On the 55th day, these 20 rats were decapitated. Mandibles of all rats were dissected out, ultrastructural evaluation was performed on the section has alveolar bone and periodontal ligament of molar teeth. The level of IL-6 in blood serum assessed using the ELISA method. Results: No healing in extracellular structure, fibroblastic activity of alveolar bone and periodontal ligament was observed by ultrastructural evaluation in Group 2 compared to those of Group I and Group 3. Significant increases in serum IL-6 levels in Group!, Group 2 and Group3 were observed (p<().00l) compared to control group. Conclusion: Ultrastructural and serum IL-6 level analysis show that oral administration of doxycycline did not induce healing of alveolar bone and periodontal ligament in experimental periodontitis in rats.

Özet

Amaç: Çalışmanın amacı ratlarda ligatür yardımıyla oluşturulan deneysel periodontitis modelinde sistemik doksisiklin uygulamasının, ultrastrüktürel olarak alveol kemiği, periodontal ligamente etkisinin ve serum interlökin-6 (IL-6) düzeyinin incelenmesidir. Materyal ve Yöntem: Çalışmada ağırlıkları 250-3()0gr olan 40 adet Wistar rat kullanıldı. 30 rat deney grubu ve 10 rat kontrol grubu olarak değerlendirildi. Ligatür yardımıyla 30 ratın mandibular molar dişlerinde periodontitis oluşturuldu. 40.günde ecardiac puncture yöntemiyle kan alınmasından sonra periodontitis oluşturulan ratlardan 10 tanesi (GrupI) ve kontrol grubu dekapite edildi. 40.günden itibaren 10 periodontitisli rata, llmglgün dozunda oval yoldan doksisiklin uygulanırken (Grııpl), geriye kalan 10 vata 15 gün herhangi bir medikasyon uygulanmadı (Grup3). 55.günde bu iki grup da, kan alınmasını takiben dekapite edildi. Tüm ratların mandibulaları çıkarıldı, ultrastrüktürel değerlendirme molar dişlerde alveol kemiği ve periodontal ligamenti içeren kesitte yapıldı. Serum IL-6 düzeyi ELISA metoduyla tayın edildi. Bulgular: Doksisiklin uygulanan grupta (Grup2), Gvupl ve GvıtpS ile karşılaştırdığımızda ultrastrüktürel olarak; alveol kemiği ve periodontal ligamentte ekstrasellüler yapı,fibroblastik aktivitede düzelmeye rastlanmadı. Grupl, Grup 2 ve Grup3 serum IL-6 seviyelerinde, kontrol grubundan farklı olarak, istatistiksel olarak anlamlı artış tespit edildi (p<0.0OI). Sonuç: Ligatürle oluşturulan deneysel peviodontitis modelinde sistemik doksisiklin uygulamasının, ultrastrüktürel ve serum IL-6 düzeyi incelemeleri sonucunda alveol kemiği ve periodontal ligamentte iyileşmeyi indüklemediği saptanmıştır.

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Cite This Article

KELEŞ, G., ÖZKAN, B., ALDIKAÇTI, M., LORTLAR, N., AÇIKGÖZ, G., FIRATLI, E. (2004). Evaluation of the systemic doxycycline therapy on alveolar bone, periodontal ligament by ultrastructural analysis and serum interleukin-6 level determination in experimental periodontitis. Ondokuz Mayıs Üniv. Diş Hekimliği Derg., Cilt: 5 - Sayı: 2, 17–21. https://ajindex.com/articles/293272

References (1)

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