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Journals / Bingöl Üniversitesi Sağlık Dergisi / 2026 / Cilt: 4 Sayı: 1

Bazı Antibiyotiklerin Koyun Dalak Glutatyon S-Transferaz Enzimi Üzerine İn Vitro Etkilerinin İncelenmesi

Ömer Faruk Çiftci(İNÖNÜ ÜNİVERSİTESİ)0000-0002-4214-4382
Songül Ünüvar(İNÖNÜ ÜNİVERSİTESİ)0000-0001-8454-490X
Yusuf Temel(BİNGÖL ÜNİVERSİTESİ)0000-0001-8148-3718
Pages
190–196
Publication Date
2023-06-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Tylosinin ise 0,545-5,45 mM aralığında enzim üzerinde herhangi etkiye sahip olmadığı belirlendi.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Glutatyon S-transferazlar (GST) hücre içi detoksifikasyon mekanizmasında görev alan multigen ailesine sahip izoenzimlerdir.
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Key Findings & Data:GST'ler, faz II sistemi ile endojen ve eksojen kaynaklı kimyasalların toksik etkisini azaltır.
💡
Conclusion & Impact:Tylosinin ise 0,545-5,45 mM aralığında enzim üzerinde herhangi etkiye sahip olmadığı belirlendi.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antibiyotik · Enzim · Glutatyon S-transferaz

Özet

Glutatyon S-transferazlar (GST) hücre içi detoksifikasyon mekanizmasında görev alan multigen ailesine sahip izoenzimlerdir. GST'ler, faz II sistemi ile endojen ve eksojen kaynaklı kimyasalların toksik etkisini azaltır. GST izoenzimleri tarafından katalize edilen reaksiyonlarda, indirgenmiş glutatyonun (GSH) tiyol grubu sayesinde çeşitli elektrofillerle konjugasyon oluşturur. GST izoenzimleri hücrede sitozol ya da mikrozomlarda bulunabilir. Bu çalışmada koyun dalak dokusundan sitozolik glutatyon S-transferaz enzimi, homojenat hazırlanması ve glutatyon agaroz afinite kromatografisi kullanılarak saflaştırıldı. İkinci aşamada saflaştırılan GST enzimi üzerine sefazolin sodyum, sefuroksim sodyum, sefaperazon sodyum, ampisillin, gentamisin, klindamisin ve tylosin antibiyotiklerin in vitro etkileri incelendi ve inhibisyon etkisi gösteren antibiyotikler için IC50 değerleri hesaplandı. Antibiyotiklerden sefaperazon sodyum, sefazolin sodyum, sefuroksim sodyum, klindamisin ve gentamisin, enzim aktivitesi üzerinde inhibisyon etkisi gösterirken ampisillinin aktivasyon etkisi gösterdiği tespit edildi. Tylosinin ise 0,545-5,45 mM aralığında enzim üzerinde herhangi etkiye sahip olmadığı belirlendi.

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Cite This Article

Çiftci, Ö. F., Ünüvar, S., Temel, Y. (2023). Bazı Antibiyotiklerin Koyun Dalak Glutatyon S-Transferaz Enzimi Üzerine İn Vitro Etkilerinin İncelenmesi. Bingöl Üniversitesi Sağlık Dergisi, Cilt: 4 Sayı: 1, 190–196. https://doi.org/10.58605/bingolsaglik.1281161

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