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Journals / Avrasya Sağlık Bilimleri Dergisi / 2025 / Cilt: 8 Sayı: 3

Glutamin Enema Might Have Helped Reversing İntestinal Mucosal İnjury Among Diarrheic Calves: Trophic Aminoacid For ‘Microbiota-Gut-Brain Axis’

Glutamin Eneması İshalli Buzağılarda Bağırsak Mukozal Hasarının Tersine Çevrilmesine Yardımcı Olmuş Olabilir: 'Mikrobiyota-Bağırsak-Beyin Ekseni' İçin Trofik Aminoasit

Deniz Alıç Ural(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, SULTANHİSAR MESLEK YÜKSEKOKULU, BİTKİSEL VE HAYVANSAL ÜRETİM BÖLÜMÜ)0000-0002-2659-3495
Pages
171–176
Publication Date
2025-09-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Highlights: Glutamine may be a repair molecule and feed additive in intestinal damage.

📌 3 Key Takeaways

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Objective & Hypothesis:Introduction: Glutamine (Gln) has been denoted as an effectual nutritional supplementation for improving the gastrointestinal barrier function of animals.
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Key Findings & Data:Materials and Methods: In a total of 20 calves with diarrhea, with unknown etiology, were enrolled at this study.
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Conclusion & Impact:Highlights: Glutamine may be a repair molecule and feed additive in intestinal damage.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:bağırsak · diamin oksidaz · glutamin

Abstract

Introduction: Glutamine (Gln) has been denoted as an effectual nutritional supplementation for improving the gastrointestinal barrier function of animals. Aim: In the present study evaluating the efficacy of Gln on intestinal morphology, as detected by Diamine oxidase (dAo), a valuable marker for detecting gastrointestinal toxicity and intestinal mucosal injury, among diarrheic calves. Materials and Methods: In a total of 20 calves with diarrhea, with unknown etiology, were enrolled at this study. Following physical examination performed by board certified veterinary surgeons (who were not involved other than technical intervention), rapid diagnostic test kits were applied without any responsible etiological agent detected, a total of 0.9 ml blood was withdrawn from each calf. Sandwich ELISA by use of commercially available bovine dAo test kits were deemed available. Glutamine (Gut-cumin I Liquid Sol.) was given via rectal route as 15 mg/kg for 7 consequtive days. Results: Mean± SE dAo (ng/mL) analysis were deemed available at days 0 (start of trial) and primary end point (day 7), presented 9.12±2.46 vs. 28.19±3.18 (p<0,01), respectively. Conclusion: It should not be unwise to draw suggestion that a trophic aminoacid, glutamine, might have helped reversing intestinal mucosal injury among diarrheic calves as detected by dAo levels. Highlights: Glutamine may be a repair molecule and feed additive in intestinal damage.

Özet

Giriş: Glutamin (Gln), hayvanların gastrointestinal bariyer fonksiyonunu iyileştirmek için etkili bir besin takviyesi olarak gösterilmiştir. Amaç: Bu çalışmada, ishalli buzağılarda gastrointestinal toksisite ve intestinal mukozal hasarı tespit etmek için değerli bir belirteç olan Diamin oksidaz (dAo) ile tespit edilen intestinal morfoloji üzerindeki Gln'nin etkinliği değerlendirilmiştir. Materyal ve Metot: Etiyolojisi bilinmeyen toplam 20 buzağı bu çalışmaya dahil edildi. Teknik müdahale dışında herhangi bir müdahalede bulunmayan kurul onaylı veteriner cerrahlar tarafından yapılan fiziksel muayenenin ardından, herhangi bir sorumlu etiyolojik ajan tespit edilmeden hızlı tanı test kitleri uygulandı ve her buzağıdan toplam 0,9 ml kan alındı. Ticari olarak temin edilebilen sığır dAo test kitleri kullanılarak sandviç ELISA'nın mevcut olduğu kabul edildi. Bulgular: Glutamin (Gut-cumin I Liquid Sol.) 7 ardışık gün boyunca rektal yoldan 15 mg/kg olarak verildi. Ortalama± SE dAo (ng/mL) analizi 0. günde (denemenin başlangıcı) ve birincil son noktada (7. gün) mevcut kabul edildi, sırasıyla 9,12±2,46'ya karşı 28,19±3,18 (p<0,01) sunuldu. Sonuç: dAo seviyeleriyle tespit edildiği gibi, ishalli buzağılarda bağırsak mukozal hasarının tersine çevrilmesine yardımcı olabilecek bir trofik aminoasit olan glutaminin önerilmesi akıllıca olmamalıdır. Öne Çıkanlar: Glutamin bağırsak hasarında tamir edici bir molekül ve yem katkı maddesi olabilir.

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Cite This Article

Ural, D. A. (2025). Glutamin Enema Might Have Helped Reversing İntestinal Mucosal İnjury Among Diarrheic Calves: Trophic Aminoacid For ‘Microbiota-Gut-Brain Axis’. Avrasya Sağlık Bilimleri Dergisi, Cilt: 8 Sayı: 3, 171–176. https://doi.org/10.53493/avrasyasbd.1711822

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References (32)

  1. Albrecht J, Sidoryk-Wegrzynowicz M, Zielinska M, Aschner M. (2010). Roles of glutamine in neurotransmission. Neuronal Glia Biol, 6 (4): 263-276. doi:10.1017/S1740925X11000093
  2. Alıç Ural D. (2024a). Investigation of diamine oxidase as a biomarker of intestinal mucosal injury in calves exposed to antibacterial treatment. J Adv VetBio Sci Tech, 9 (3): 190- 196. doi:10.31797/vetbio.1423891
  3. Alıç Ural D. (2024b). Intestinal mucosal damage and intestinal permeability in non-infectious and infectious diarrheic calves in relation to diamine oxidase activity. Int J Vet Anim Res, 7 (3): 83-86. doi:10.5281/zenodo.14583367
  4. Alıç Ural D, Erdoğan S, Kılıç N, Erdoğan H, Turk E, Ural K. (2023). Probiotic enema protects intestinal mucosae and decreases plasma diamine oxidase activity among calves with diarrhea. Veterinaria, 72 (3): 283-289.
  5. Alizadeh, A, Akbari P, Garssen J, Fink-Gremmels J, Braber S. (2022). Epithelial integrity, junctional complexes, and biomarkers associated with intestinal functions. Tissue Barriers, 10(3), 1996830.
  6. Amores-Sanchez MI, Medina MA. (1999). Glutamine, as a precursor of glutathione, and oxidative stress. Mol Genet Metab, 67 (2): 100-105. doi:10.1006/mgme.1999.2857
  7. Avşar FM, Şahin M, Saygun O, Özel İH, Hengirmen S, Vatansev H, Kafalı E, Yılmaz O. (2001). Effects of oral L-glutamine, insulin and laxative on the severity of acute pancreatitis. Turk J Med Sci, 31 (6): 555-560.
  8. Brooks HW, White DG, Wagstaff AJ, Michell AR. (1996). Evaluation of a nutritive oral rehydration solution for the treatment of calf diarrhea. Br Vet J, 152 (6): 699-708. doi:10.1016/S0007-1935(96)80112-0
  9. Buffoni F. (1966). Histaminase and related amine oxidases. Pharmacol Rev, 18 (4): 1163-1199. doi:10.1124/pr.18.4.1163
  10. Calder P. (1994). Glutamine and the immune system. Clin Nutr, 13 (1): 2-8. doi:10.1016/0261-5614(94)90003-5
  11. Ceja G, Boerman JP, Neves RC, Jorgensen MW, Johnson JS. (2023). L-glutamine supplementation reduces gastrointestinal permeability and biomarkers of physiological stress in preweaning Holstein heifer calves. J Dairy Sci, 106 (12): 9663-9676. doi:10.3168/jds.2023-23456
  12. Coster J, McCauley R, Hall J. (2004). Glutamine: Metabolism and application in nutrition support. Asia Pac J Clin Nutr, 13: 25-31.
  13. D'Agostino L, D'Argenio G, Ciacci C, Daniele B, Macchia V, Mazzacca G. (1984). Diamine oxidase in rat small bowel: Distribution in different segments and cellular location. Enzyme, 31 (4): 217-220. doi:10.1159/000469529
  14. DeBell RM, Ledney GD, Snyder SL. (1987). Quantification of gut injury with diamine oxidase activity: development of a fission neutron RBE and measurements with combined injury in mouse models. Radiat Res, 112(3):508-516.
  15. Dong X, Zhai R, Liu Z, Lin X, Wang Z, Hu Z. (2019). The effect of intravenous infusions of glutamine on duodenal cell autophagy and apoptosis in early-weaned calves. Animals (Basel), 9 (7): 404. doi:10.3390/ani9070404
  16. Fukuda T, Tsukano K, Otsuka M, Murakami Y, Kitade Y, Nakatsuji H, Sera K, Suzuki K. (2020). Relationship between postnatal days, serum Cu concentration and plasma diamine oxidase activity in Japanese Black calves. J Vet Med Sci, 82 (10): 1488-1491. doi:10.1292/jvms.20-0200
  17. Fukudome I, Kobayashi M, Dabanaka K, Maeda H, Okamoto K, Okabayashi T, Baba R, Kumagai N, Oba K, Fujita M, Hanazaki K. (2014). Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats. Med Mol Morphol, 47 (2): 100-107. doi:10.1007/s00795-013-0060-0
  18. Karinch AM, Pan M, Lin CM, Strange R, Souba WW. (2001). Glutamine metabolism in sepsis and infection. J Nutr, 131 (9 Suppl): 2535S–2538S. doi:10.1093/jn/131.9.2535S
  19. Kaya E, Ceylan A, Kara N, Güven H, Yıldız L. (2007). The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. Turk J Gastroenterol, 18 (2): 89–94.
  20. Lacey JM, Wilmore DW. (1990). Is glutamine a conditionally essential amino acid? Nutr Rev, 48 (8): 297–309. doi:10.1111/j.1753-4887.1990.tb02967.x
  21. Larson LL, Owen FG, Albright JL, Appleman RD, Lamb RC, Muller LD. (1977). Guidelines toward more uniformity in measuring and reporting calf experimental data. J Dairy Sci, 60 (6): 989–991. doi:10.3168/jds.S0022-0302(77)83975-1
  22. Luk GD, Bayless TM, Baylin SB. (1980). Diamine oxidase (histaminase): A circulating marker for rat intestinal mucosal maturation and integrity. J Clin Invest, 66 (1): 66– 70. doi:10.1172/JCI109836
  23. McGuirk SM. (2008). Disease management of dairy calves and heifers. Vet Clin North Am Food Anim Pract, 24 (1): 139–153. doi:10.1016/j.cvfa.2007.10.003
  24. Newsholme P. (2001). Why is L-glutamine metabolism important to cells of the immune system in health, postinjury, surgery or infection? J Nutr, 131 (9 Suppl): 2515S–2522S. doi:10.1093/jn/131.9.2515S
  25. Newsholme P, Curi R, Pithon-Curi TC, Murphy CJ, Garcia C, Pires de Melo M. (1999). Glutamine metabolism by lymphocytes, macrophages, and neutrophils: Its importance in health and disease. J Nutr Biochem, 10 (6): 316–324. doi:10.1016/ S0955-2863(99)00022-4
  26. Ono M, Ishii A, Aritsune M, Horikita T. (2023). Changes in serum concentration and activity of diamine oxidase during the first month of life in calves. J Vet Med Sci, 85 (4): 417–419. doi:10.1292/jvms.22-0343
  27. Patience JF. (1990). A review of the role of acid-base balance in amino acid nutrition. J Anim Sci, 68 (2): 398–408. doi:10.2527/1990.682398x
  28. Shakir KM, Margolis S, Baylin SB. (1977). Localization of histamine (diamine oxidase) in rat small intestinal mucosa: Site of release by heparin. Biochem Pharmacol, 26 (23): 2343–2347. doi:10.1016/0006-2952(77)90438-5
  29. Song Z, Cheng K, Zhang L, Wang T. (2017). Dietary supplementation of enzymatically treated Artemisia annua could alleviate the intestinal inflammatory response in heat-stressed broilers. J Therm Bio, 69: 184-190.
  30. Wang S, Wang F, Kong F, Cao Z, Wang W, Yang H, Wang Y, Bi Y, Li S. (2022). Effect of supplementing different levels of L-glutamine on Holstein calves during weaning. Antioxidants, 11 (3): 542. doi:10.3390/antiox11030542
  31. Wolvekamp MC, de Bruin RW. (1994). Diamine oxidase: An overview of historical, biochemical and functional aspects. Dig Dis, 12 (1): 2–14. doi:10.1159/000171439
  32. Zhou Y, Zhang P, Deng G, Liu X, Lu D. (2012). Improvements of immune status, intestinal integrity, and gain performance in the early-weaned calves parenterally supplemented with L-alanyl-L-glutamine dipeptide. Vet Immunol Immunopathol, 145 (1–2): 134–142. doi:10.1016/j. vetimm.2011.10.020