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Journals / Animal Health Production and Hygiene / 2025 / Cilt: 14 Sayı: 2

Effect of ATP Addition to Goat Semen Post Thaw on Semen Parameters

Çözüm sonu teke spermasına ATP ilavesinin sperma parametreleri üzerine etkisi

Uğur Uçan(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0001-8325-138X
Zahid Naseer(Pir Mehr Ali Shah Arid Agriculture University)0000-0001-6335-9633
Melih Aksoy(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0001-9573-4493
Pages
46–51
Publication Date
2025-12-25
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that ATP supplementation might restore reduced motility problem, at least partly, in goat spermatozoa resulting from cryopreservation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In post-thaw goat spermatozoa the impact of ATP supplementation at different dose levels on motility and the other sperm parameters was evaluated during 4 hours of incubation.
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Key Findings & Data:The ejaculate was frozen in 0.25ml straws by using egg yolk-glucose-glycerol-tris-citric acid extender.
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Conclusion & Impact:It was concluded that ATP supplementation might restore reduced motility problem, at least partly, in goat spermatozoa resulting from cryopreservation.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:akrozom reaksiyonu · ATP · Çözüm sonu

Abstract

In post-thaw goat spermatozoa the impact of ATP supplementation at different dose levels on motility and the other sperm parameters was evaluated during 4 hours of incubation. The ejaculate was frozen in 0.25ml straws by using egg yolk-glucose-glycerol-tris-citric acid extender. Thawing was achieved at 37°C for 30 second in a water bath and sperm cells were rediluted, following the removal of the extender, in tris-citric acid-glucose solution before adding 0 (control), 2, 4 or 8 mM of ATP. Eight of 10 parameters that were examined by the computer assisted sperm analyzer has been influenced by ATP supplementation (P 0.05) by ATP supplementation irrespective to the dose levels. As a result, ATP supplementation (2-4mM) to post-thaw goat spermatozoa improved progressive motility during the incubation (P<0.05) without any prominent influence on most of the kinematic sperm parameters including ALH, VCL, VAP and VSL. Further increase the ATP concentrations to 8mM suppressed motility parameters significantly (P<0.01). It was concluded that ATP supplementation might restore reduced motility problem, at least partly, in goat spermatozoa resulting from cryopreservation.

Özet

Çözüm sonrası teke spermatozoonlarına farklı doz seviyelerinde ATP takviyesinin motilite ve diğer sperm parametreleri üzerindeki etkisi 4 saatlik inkübasyon süresince değerlendirildi. Ejekülat, yumurta sarısı-glikoz-gliserol-tris-sitrik asit sulandırıcısı kullanılarak 0.25 ml payetlerde donduruldu. Su banyosunda 30 saniye süreyle 37°C'de payetler çözüldü ve sperm hücreleri, sulandırıcı içeriklerinin uzaklaştırılmasının ardından 0 (kontrol), 2, 4 veya 8 mM ATP eklenmeden önce tris-sitrik asit-glikoz çözeltisi içerisinde yeniden seyreltildi. Spermatolojik parametrelerin büyük çoğunluğu ATP takviyesinden (P 0.05). Sonuç olarak, çözüm sonrası teke spermatozoasına ATP takviyesi (2-4 mM), ALH, VCL, VAP ve VSL dahil olmak üzere kinematik sperm parametrelerinin çoğu üzerinde belirgin bir etki olmaksızın inkübasyon periyodu boyunca progresif motiliteyi geliştirdi (P<0.05). ATP konsantrasyonunun 8 mM seviyesine çıkarılması motilite parametrelerini önemli ölçüde düşürdü. (P<0.01). ATP takviyesinin, teke spermatozoasında kriyoprezervasyondan kaynaklanan azalmış motilite problemini en azından kısmen düzeltebileceği sonucuna varıldı.

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Cite This Article

Uçan, U., Naseer, Z., Aksoy, M. (2025). Effect of ATP Addition to Goat Semen Post Thaw on Semen Parameters. Animal Health Production and Hygiene, Cilt: 14 Sayı: 2, 46–51. https://doi.org/10.53913/aduveterinary.1809432

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