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Dergiler / Animal Health Production and Hygiene / 2025 / Cilt: 14 Sayı: 2

Çözüm sonu teke spermasına ATP ilavesinin sperma parametreleri üzerine etkisi

Effect of ATP Addition to Goat Semen Post Thaw on Semen Parameters

Uğur Uçan(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0001-8325-138X
Zahid Naseer(Pir Mehr Ali Shah Arid Agriculture University)0000-0001-6335-9633
Melih Aksoy(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0001-9573-4493
Sayfa
46–51
Yayın Tarihi
2025-12-25
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, çözüm sonrası teke spermatozoasına ATP takviyesi (2-4 mM), ALH, VCL, VAP ve VSL dahil olmak üzere kinematik sperm parametrelerinin çoğu üzerinde belirgin bir etki olmaksızın inkübasyon periyodu boyunca progresif motiliteyi geliştirdi (P<0.05).

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Amaç & Hipotez:Çözüm sonrası teke spermatozoonlarına farklı doz seviyelerinde ATP takviyesinin motilite ve diğer sperm parametreleri üzerindeki etkisi 4 saatlik inkübasyon süresince değerlendirildi.
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Temel Bulgular & Veri:Ejekülat, yumurta sarısı-glikoz-gliserol-tris-sitrik asit sulandırıcısı kullanılarak 0.25 ml payetlerde donduruldu.
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Sonuç & Katkı:Sonuç olarak, çözüm sonrası teke spermatozoasına ATP takviyesi (2-4 mM), ALH, VCL, VAP ve VSL dahil olmak üzere kinematik sperm parametrelerinin çoğu üzerinde belirgin bir etki olmaksızın inkübasyon periyodu boyunca progresif motiliteyi geliştirdi (P<0.05).
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:akrozom reaksiyonu · ATP · Çözüm sonu

Özet

Çözüm sonrası teke spermatozoonlarına farklı doz seviyelerinde ATP takviyesinin motilite ve diğer sperm parametreleri üzerindeki etkisi 4 saatlik inkübasyon süresince değerlendirildi. Ejekülat, yumurta sarısı-glikoz-gliserol-tris-sitrik asit sulandırıcısı kullanılarak 0.25 ml payetlerde donduruldu. Su banyosunda 30 saniye süreyle 37°C'de payetler çözüldü ve sperm hücreleri, sulandırıcı içeriklerinin uzaklaştırılmasının ardından 0 (kontrol), 2, 4 veya 8 mM ATP eklenmeden önce tris-sitrik asit-glikoz çözeltisi içerisinde yeniden seyreltildi. Spermatolojik parametrelerin büyük çoğunluğu ATP takviyesinden (P 0.05). Sonuç olarak, çözüm sonrası teke spermatozoasına ATP takviyesi (2-4 mM), ALH, VCL, VAP ve VSL dahil olmak üzere kinematik sperm parametrelerinin çoğu üzerinde belirgin bir etki olmaksızın inkübasyon periyodu boyunca progresif motiliteyi geliştirdi (P<0.05). ATP konsantrasyonunun 8 mM seviyesine çıkarılması motilite parametrelerini önemli ölçüde düşürdü. (P<0.01). ATP takviyesinin, teke spermatozoasında kriyoprezervasyondan kaynaklanan azalmış motilite problemini en azından kısmen düzeltebileceği sonucuna varıldı.

Abstract

In post-thaw goat spermatozoa the impact of ATP supplementation at different dose levels on motility and the other sperm parameters was evaluated during 4 hours of incubation. The ejaculate was frozen in 0.25ml straws by using egg yolk-glucose-glycerol-tris-citric acid extender. Thawing was achieved at 37°C for 30 second in a water bath and sperm cells were rediluted, following the removal of the extender, in tris-citric acid-glucose solution before adding 0 (control), 2, 4 or 8 mM of ATP. Eight of 10 parameters that were examined by the computer assisted sperm analyzer has been influenced by ATP supplementation (P 0.05) by ATP supplementation irrespective to the dose levels. As a result, ATP supplementation (2-4mM) to post-thaw goat spermatozoa improved progressive motility during the incubation (P<0.05) without any prominent influence on most of the kinematic sperm parameters including ALH, VCL, VAP and VSL. Further increase the ATP concentrations to 8mM suppressed motility parameters significantly (P<0.01). It was concluded that ATP supplementation might restore reduced motility problem, at least partly, in goat spermatozoa resulting from cryopreservation.

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Bu Makaleye Atıf Yap

Uçan, U., Naseer, Z., Aksoy, M. (2025). Çözüm sonu teke spermasına ATP ilavesinin sperma parametreleri üzerine etkisi. Animal Health Production and Hygiene, Cilt: 14 Sayı: 2, 46–51. https://doi.org/10.53913/aduveterinary.1809432

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