SavedAdd your journal

Journals / Animal Health Production and Hygiene / 2026 / Cilt: 15 Sayı: 4

Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats

Streptozotosin ile Diyabet Oluşturulan Sıçanlarda Farklı Dozlarda Arı Sütünün Oksidatif Stres Parametreleri Üzerine Etkileri

Hande Sultan Şahiner(ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY)0000-0003-2811-3579
Mehmet Onur Ak(ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY)0000-0002-2562-857X
Özge Bardakçı Yılmaz(AYDIN ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE)0000-0001-5855-600X
Merve Avcıoğlu(ADNAN MENDERES UNIVERSITY, INSTITUTE OF HEALTH SCIENCES)0000-0003-2061-4395
Sultan Gündeş Kut(ADNAN MENDERES UNIVERSITY, INSTITUTE OF HEALTH SCIENCES)0000-0002-6076-0314
Selim Sekkin(ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY)0000-0002-3795-5375
Murat Boyacıoğlu(ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY)0000-0001-6952-8637
Cavit Kum(ADNAN MENDERES UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY)0000-0002-4032-3950
Pages
—
Publication Date
2026-10-27
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results indicate that royal jelly did not clearly suppress lipid peroxidation but may modestly limit the diabetes-related decline in antioxidant defense.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Chronic hyperglycemia in diabetes increases the cellular oxidant burden while weakening antioxidant defense capacity.
📊
Key Findings & Data:Thirty-six adult male Sprague–Dawley rats were allocated to healthy control, diabetic control, healthy royal jelly (300 mg/kg), and diabetic royal jelly groups receiving 50, 150, or 300 mg/kg.
💡
Conclusion & Impact:These results indicate that royal jelly did not clearly suppress lipid peroxidation but may modestly limit the diabetes-related decline in antioxidant defense.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Arı sütü · diyabet · malondialdehit

Abstract

Chronic hyperglycemia in diabetes increases the cellular oxidant burden while weakening antioxidant defense capacity. Royal jelly has been suggested to exert antioxidant effects; however, its tissue-specific activity at different doses remains unclear. The present study investigated malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activities in serum, brain, kidney, heart, and testis samples obtained from male rats with streptozotocin (STZ)-induced diabetes after royal jelly treatment. Thirty-six adult male Sprague–Dawley rats were allocated to healthy control, diabetic control, healthy royal jelly (300 mg/kg), and diabetic royal jelly groups receiving 50, 150, or 300 mg/kg. A 50 mg/kg dose of STZ was administered intraperitoneally to establish diabetes, and royal jelly was given orally once daily for four weeks. Biochemical measurements were performed spectrophotometrically. Serum MDA concentrations were comparable among groups, whereas serum SOD activity showed a significant group effect (p = 0.005), with lower activity in diabetic controls than in the healthy control and healthy royal jelly groups. Renal MDA was elevated and renal SOD activity was reduced in diabetic controls relative to the healthy groups (p 0.05). Royal jelly did not significantly reduce renal MDA; nevertheless, SOD activities in serum, kidney, and heart tended to be higher in treated diabetic groups than in diabetic controls and were comparable to healthy control values. These results indicate that royal jelly did not clearly suppress lipid peroxidation but may modestly limit the diabetes-related decline in antioxidant defense.

Özet

Diyabette kronik hiperglisemi, hücresel oksidan yükü artırırken antioksidan savunma kapasitesini zayıflatır. Arı sütünün antioksidan etkilere sahip olabileceği bildirilmiş olmakla birlikte, farklı dozlarda dokuya özgü etkileri yeterince açıklığa kavuşmamıştır. Bu çalışmada, streptozotosin (STZ) ile diyabet oluşturulan erkek sıçanlarda arı sütü uygulaması sonrasında serum, beyin, böbrek, kalp ve testis örneklerindeki malondialdehit (MDA) düzeyleri ile süperoksit dismutaz (SOD) aktiviteleri araştırıldı. Otuz altı erişkin erkek Sprague–Dawley sıçan; sağlıklı kontrol, diyabetik kontrol, sağlıklı arı sütü (300 mg/kg) ve 50, 150 veya 300 mg/kg arı sütü uygulanan diyabetik gruplara ayrıldı. Diyabet oluşturmak amacıyla STZ, 50 mg/kg dozunda intraperitoneal olarak uygulandı ve arı sütü dört hafta boyunca günde bir kez oral yolla verildi. Biyokimyasal ölçümler spektrofotometrik olarak yapıldı. Serum MDA düzeyleri gruplar arasında benzerken, serum SOD aktivitesinde anlamlı grup etkisi saptandı (p = 0,005); diyabetik kontrol grubundaki aktivite sağlıklı kontrol ve sağlıklı arı sütü gruplarından daha düşüktü. Diyabetik kontrolde böbrek MDA düzeyi yükselmiş, böbrek SOD aktivitesi azalmıştı (sırasıyla p 0,05). Arı sütü böbrek MDA düzeyini anlamlı olarak azaltmadı; ancak serum, böbrek ve kalp SOD aktiviteleri tedavi edilen diyabetik gruplarda diyabetik kontrolden sayısal olarak daha yüksek ve sağlıklı kontrol değerlerine yakındı. Bulgular, arı sütünün lipid peroksidasyonunu belirgin biçimde baskılamadığını, ancak diyabete bağlı antioksidan savunma kaybını sınırlı ölçüde azaltabileceğini göstermektedir.

🔖

Cite This Article

Şahiner, H. S., Ak, M. O., Yılmaz, Ö. B., Avcıoğlu, M., Kut, S. G., Sekkin, S., Boyacıoğlu, M., Kum, C. (2026). Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats. Animal Health Production and Hygiene, Cilt: 15 Sayı: 4. https://doi.org/10.53913/aduveterinary.2012739

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (31)

  1. Asmat, U., Abad, K., & Ismail, K. (2016). Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal, 24(5), 547-553. https://doi.org/10.1016/j.jsps.2015.03.013
  2. Botezan, S., Baci, G.M., Bagameri, L., Pașca, C., & Dezmirean, D.S. (2023). Current status of the bioactive properties of royal jelly: A comprehensive review with a focus on its anticancer, antiinflammatory, and antioxidant effects. Molecules, 28(3), Article 1510. https://doi.org/10.3390/molecules28031510
  3. Del Rio, D., Stewart, A.J., & Pellegrini, N. (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, Metabolism and Cardiovascular Diseases, 15(4), 316-328. https://doi.org/10.1016/j.numecd.2005.05.003
  4. Dena, S.M., Adeleye, A.O., Mohlala, K., Langa, B.C., & Opuwari, C.S. (2025). The impact of diabetes mellitus-related oxidative stress on male fertility: A review. Journal of Diabetes, 17(10), Article e70157. https://doi.org/10.1111/1753-0407.70157
  5. Feng, C., Song, J., Deng, L., Zhang, J., Lian, X., Zhen, Z., & Liu, J. (2024). Ginsenoside Rb1 reduces oxidative/carbonyl stress damage and dysfunction of RyR2 in the heart of streptozotocin-induced diabetic rats. BMC Cardiovascular Disorders, 24(1), Article 333. https://doi.org/10.1186/s12872-024-04005-8
  6. Furman, B.L. (2021). Streptozotocin-induced diabetic models in mice and rats. Current Protocols, 1, Article e78. https://doi.org/10.1002/cpz1.78
  7. Ghanbari, E., Nejati, V., & Azadbakht, M. (2015a). Protective effect of royal jelly against renal damage in streptozotocin-induced diabetic rats. Iranian Journal of Toxicology, 9(28), 1258-1263.
  8. Ghanbari, E., Nejati, V., Najafi, G., Khazaei, M., & Babaei, M. (2015b). Study on the effect of royal jelly on reproductive parameters in streptozotocin-induced diabetic rats. International Journal of Fertility & Sterility, 9(1), 113-120. https://doi.org/10.22074/ijfs.2015.4215
  9. Ghanbari, E., Nejati, V., & Khazaei, M. (2016). Antioxidant and protective effects of royal jelly on histopathological changes in testis of diabetic rats. International Journal of Reproductive BioMedicine, 14(8), 519-526. https://doi.org/10.29252/ijrm.14.8.519
  10. Ghasemi, A., & Jeddi, S. (2023). Streptozotocin as a tool for induction of rat models of diabetes: A practical guide. EXCLI Journal, 22, 274– 294. https://doi.org/10.17179/excli2022-5720
  11. Guo, H., Ekusa, A., Iwai, K., Yonekura, M., Takahata, Y., & Morimatsu, F. (2008). Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo. Journal of Nutritional Science and Vitaminology, 54(3), 191- 195. https://doi.org/10.3177/jnsv.54.191
  12. Guo, Z., Xia, Z., Jiang, J., & McNeill, J.H. (2007). Down-regulation of NADPH oxidase, antioxidant enzymes, and inflammatory markers in the heart of streptozotocin-induced diabetic rats by N-acetylcysteine. American Journal of Physiology-Heart and Circulatory Physiology, 292(4), H1728-H1736. https://doi.org/10.1152/ajpheart.01328.2005
  13. Islam, M.N., Rauf, A., Fahad, F.I., Emran, T.B., Mitra, S., Olatunde, A.,. Mubarak, M.S. (2022). Superoxide dismutase: An updated review on its health benefits and industrial applications. Critical Reviews in Food Science and Nutrition, 62(26), 7282-7300. https://doi.org/10.1080/10408398.2021.1913400
  14. Karaca, T., Demirtaş, S., Karaboğa, İ., & Ayvaz, S. (2015). Protective effects of royal jelly against testicular damage in streptozotocininduced diabetic rats. Turkish Journal of Medical Sciences, 45(1), 27-32.
  15. Kuru Karabaş, M., Ayhan, M., Güney, E., Serter, M., & Meteoğlu, İ. (2013). The effect of pioglitazone on antioxidant levels and renal histopathology in streptozotocin-induced diabetic rats. ISRN Endocrinology, 2013, Article 858690. https://doi.org/10.1155/2013/858690
  16. Malekinejad, H., Ahsan, S., Delkhosh-Kasmaie, F., Cheraghi, H., Rezaei- Golmisheh, A., & Janbaz-Acyabar, H. (2016). Cardioprotective effect of royal jelly on paclitaxel-induced cardiotoxicity in rats. Iranian Journal of Basic Medical Sciences, 19(2), 221-227. https://doi.org/10.22038/ijbms.2016.6549
  17. Mostafa, R.E., El-Marasy, S.A., Abdel Jaleel, G.A., & Bakeer, R.M. (2020). Protective effect of royal jelly against diclofenac-induced hepatorenal damage and gastrointestinal ulcerations in rats. Heliyon, 6(2), Article e03330. https://doi.org/10.1016/j.heliyon.2020.e03330
  18. Naderi, R., Mohaddes, G., Mohammadi, M., Ghaznavi, R., Ghyasi, R., & Vatankhah, A.M. (2015). Voluntary exercise protects heart from oxidative stress in diabetic rats. Advanced Pharmaceutical Bulletin, 5(2), 231-236. https://doi.org/10.15171/apb.2015.032
  19. Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  20. Omer, A.B., Afzal, O., Altamimi, A.S.A., Patil, S., AlGhamdi, S.A., Alghamdi, A.M.,. Kazmi, I. (2023). Neuroprotective effect of barbaloin on streptozotocin-induced cognitive dysfunction in rats via inhibiting cholinergic and neuroinflammatory cytokines pathway-TNF-α/IL-1β/IL-6/NF-κB. ACS Omega, 8(8), 8110-8118. https://doi.org/10.1021/acsomega.2c08277
  21. Oršolić, N., & Jazvinšćak Jembrek, M. (2024). Royal jelly: Biological action and health benefits. International Journal of Molecular Sciences, 25(11), Article 6023. https://doi.org/10.3390/ijms25116023
  22. Rahmani, A.H., Alsahli, M.A., Khan, A.A., & Almatroodi, S.A. (2023). Quercetin, a plant flavonol, attenuates diabetic complications, renal tissue damage, renal oxidative stress and inflammation in streptozotocin-induced diabetic rats. Metabolites, 13(1), Article 130. https://doi.org/10.3390/metabo13010130
  23. Sabahi, Z., Khoshnoud, M.J., Hosseini, S., Khoshraftar, F., & Rashedinia, M. (2021). Syringic acid attenuates cardiomyopathy in streptozotocin-induced diabetic rats. Advances in Pharmacological and Pharmaceutical Sciences, 2021, Article 5018092. https://doi.org/10.1155/2021/5018092
  24. Sekkin, S., İpek, E.D., Boyacıoğlu, M., Kum, C., Karademir, Ü., Yalınkılınç, H.S.,. Başaloğlu, H. (2015). DNA protective and antioxidative effects of melatonin in streptozotocin-induced diabetic rats. Turkish Journal of Biology, 39(6), 932-940. https://doi.org/10.3906/biy-1507-55
  25. Sun, Y., Oberley, L.W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34(3), 497-500. https://doi.org/10.1093/clinchem/34.3.497
  26. Supabphol, R., & Nusuetrong, P. (2002). Effect of royal jelly to lipid peroxidation in diabetic rats. Revista de Fitoterapia, 2(Suppl. 1), 114.
  27. Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & PérezÁlvarez, J.A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of Food Science, 73(9), R117-R124. https://doi.org/10.1111/j.1750-3841.2008.00966.x
  28. Ye, Z., Wang, S., Wan, Z., Huang, B., & Guo, J. (2025). Targeting NADPH oxidase-driven oxidative stress in diabetic cardiomyopathy: Mechanisms and therapeutic perspectives. Frontiers in Pharmacology, 16, Article 1610429. https://doi.org/10.3389/fphar.2025.1610429
  29. Islam, M.N., Rauf, A., Fahad, F.I., Emran, T.B., Mitra, S., Olatunde, A.,. Mubarak, M.S. (2022). Superoxide dismutase: An updated review on its health benefits and industrial applications. Critical Reviews in Food Science and Nutrition, 62(26), 7282-7300. https://doi.org/10.1080/10408398.2021.1913400
  30. Omer, A.B., Afzal, O., Altamimi, A.S.A., Patil, S., AlGhamdi, S.A., Alghamdi, A.M.,. Kazmi, I. (2023). Neuroprotective effect of barbaloin on streptozotocin-induced cognitive dysfunction in rats via inhibiting cholinergic and neuroinflammatory cytokines pathway-TNF-α/IL-1β/IL-6/NF-κB. ACS Omega, 8(8), 8110-8118. https://doi.org/10.1021/acsomega.2c08277
  31. Sekkin, S., İpek, E.D., Boyacıoğlu, M., Kum, C., Karademir, Ü., Yalınkılınç, H.S.,. Başaloğlu, H. (2015). DNA protective and antioxidative effects of melatonin in streptozotocin-induced diabetic rats. Turkish Journal of Biology, 39(6), 932-940. https://doi.org/10.3906/biy-1507-55