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Journals / Animal Health Production and Hygiene / 2025 / Cilt: 14 Sayı: 2

Determination of Dynamic Thiol Disulfide Homeostasis in Cows With Dystocia

Güç Doğum Yapan İneklerde Dinamik Tiyol-Disülfid Homeostazının Belirlenmesi

Oğuz Merhan(KAFKAS UNIVERSITY)0000-0002-3399-0667
Lale Başer(kafkas üniversitesi)0000-0003-0659-6346
Kadir Bozukluhan(KAFKAS UNIVERSITY)0000-0003-4929-5156
Abdulsamed Kükürt(KAFKAS UNIVERSITY)0000-0002-3603-0506
Necdet Cankat Lehimcioğlu(KAFKAS UNIVERSITY)0000-0001-8780-616X
Emine Atakişi(KAFKAS UNIVERSITY)0000-0002-5685-1870
Ömer Çelik(KAFKAS UNIVERSITY)0000-0002-4774-5670
Pages
40–45
Publication Date
2025-12-25
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings indicate that thiol/disulfide homeostasis is disrupted in cows experiencing dystocia, reflecting severe oxidative stress and cellular redox imbalance.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Pregnancy is a complex physiological process in animals that affects multiple mechanisms and leads to oxidative stress due to the increased production of free radicals.
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Key Findings & Data:A total of 30 Simmental cows were included, comprising 15 cows with normal calving and 15 cows with dystocia.
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Conclusion & Impact:These findings indicate that thiol/disulfide homeostasis is disrupted in cows experiencing dystocia, reflecting severe oxidative stress and cellular redox imbalance.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antioksidanlar · Distosia (Güç Doğum) · İnekler

Abstract

Pregnancy is a complex physiological process in animals that affects multiple mechanisms and leads to oxidative stress due to the increased production of free radicals. Thiols, which contain sulfhydryl (-SH) groups, play a critical role in sulfur-based biochemical processes and act as potent antioxidants that protect cells from oxidative damage. The aim of this study was to investigate thiol/disulfide balance in cows experiencing dystocia. A total of 30 Simmental cows were included, comprising 15 cows with normal calving and 15 cows with dystocia. Blood samples were collected in non-anticoagulant tubes immediately after parturition. Biochemical analyses revealed that cows with dystocia had significantly lower levels of total thiol, native thiol, and native thiol/total thiol ratio compared to cows with normal calving, whereas disulfide levels were markedly elevated. Moreover, the disulfide/native thiol and disulfide/total thiol ratios were significantly higher in the dystocia group. These findings indicate that thiol/disulfide homeostasis is disrupted in cows experiencing dystocia, reflecting severe oxidative stress and cellular redox imbalance. The results suggest that thiol/disulfide homeostasis could serve as a biomarker for oxidative disturbances associated with dystocia. Furthermore, antioxidant strategies aimed at reducing oxidative stress may complement existing clinical interventions and improve recovery in affected animals. In conclusion, this study highlights the critical importance of thiol/disulfide balance during the parturition process and emphasizes its potential both as a diagnostic marker and as a target for therapeutic strategies aimed at mitigating oxidative stress. It is concluded that further research is warranted to elucidate the mechanistic links between redox imbalance and dystocia and to evaluate the effectiveness of antioxidant support in clinical applications.

Özet

Gebelik, hayvanlarda birçok mekanizmayı etkileyen karmaşık bir fizyolojik süreçtir ve serbest radikallerin artmış üretimine bağlı olarak oksidatif strese yol açmaktadır. Sülfhidril (-SH) grubu içeren tiyoller, kükürt temelli biyokimyasal süreçlerde kritik görevler üstlenir ve hücreleri oksidatif hasara karşı koruyan güçlü antioksidanlardır. Bu çalışmanın amacı, güç doğum (distosia) yapan ineklerde tiyol/disülfid dengesini incelemektir. Çalışmaya 15 normal doğum yapan ve 15 güç doğum yapan olmak üzere toplam 30 Simmental ırkı inek dahil edilmiştir. Doğumdan hemen sonra antikoagülansız tüplere kan örnekleri alınmıştır. Biyokimyasal analizler sonucunda, güç doğum yapan ineklerde toplam tiyol, doğal (native) tiyol ve doğal tiyol/toplam tiyolüzeylerinin normal doğum yapanlara göre anlamlı derecede azaldığı; buna karşılık disülfid seviyelerinin belirgin şekilde yükseldiği saptanmıştır. Ayrıca disülfid/doğal tiyol ve disülfid/toplam tiyol oranlarının da güç doğum yapan grupta belirgin derecede arttığı görülmüştür. Bu bulgular, güç doğum yapan ineklerde tiyol/disülfid dengesinin bozulduğunu ve bunun şiddetli oksidatif stres ile hücresel redoks dengesizliğini yansıttığını göstermektedir. Çalışma sonuçları, tiyol/disülfid homeostazının güç doğuma bağlı oksidatif bozuklukların belirlenmesinde biyobelirteç olarak kullanılabileceğini düşündürmektedir. Ayrıca oksidatif stresin azaltılmasına yönelik antioksidan yaklaşımlar, mevcut klinik uygulamalara destek sağlayarak etkilenen hayvanlarda iyileşmeyi artırabilir. Sonuç olarak, bu araştırma tiyol/disülfid dengesinin doğum sürecindeki kritik önemini ortaya koymakta ve hem tanısal bir belirteç hem de oksidatif stresin azaltılmasına yönelik tedavi stratejileri için bir hedef olabileceğini vurgulamaktadır. Redoks dengesizliği ile güç doğum arasındaki mekanizmal bağlantıların daha ayrıntılı araştırılması ve antioksidan desteğinin klinik uygulamalardaki etkinliğinin değerlendirilmesi gerektiği sonucuna varılmıştır.

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Cite This Article

Merhan, O., Başer, L., Bozukluhan, K., Kükürt, A., Lehimcioğlu, N. C., Atakişi, E., Çelik, Ö. (2025). Determination of Dynamic Thiol Disulfide Homeostasis in Cows With Dystocia. Animal Health Production and Hygiene, Cilt: 14 Sayı: 2, 40–45. https://doi.org/10.53913/aduveterinary.1790327

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