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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2026 / Volume: 73 Issue: 3

Growth performance, blood antioxidant status, and bone biomechanical properties in Japanese quail (Coturnix coturnix japonica) fed diets supplemented with sodium bentonite

Özlem Durna(DİCLE ÜNİVERSİTESİ)0000-0003-4532-6795
Kadir Önk(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0002-5618-2988
Gültekin Yıldız(ANKARA ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0002-1003-9251
Oğuz Merhan(KAFKAS ÜNİVERSİTESİ)0000-0002-3399-0667
Fatma Kübra Erbay Elibol0000-0002-4117-1098
Pages
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Publication Date
2026-07-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study demonstrates that sodium bentonite improves antioxidant status in quail but has no significant effects on growth performance or bone biomechanical properties, suggesting that this clay mineral may be a safe feed additive when used in a controlled manner.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Few studies have established a relationship between clay minerals used in poultry nutrition and both the antioxidant capacity and the bone biomechanical properties.
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Key Findings & Data:In our study, the effect of sodium bentonite clay mineral on growth performance, blood antioxidant status and bone biomechanical properties of quails (Coturnix coturnix japonica) was investigated.
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Conclusion & Impact:This study demonstrates that sodium bentonite improves antioxidant status in quail but has no significant effects on growth performance or bone biomechanical properties, suggesting that this clay mineral may be a safe feed additive when used in a controlled manner.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Biomechanical properties · growth performance · quails

Abstract

Few studies have established a relationship between clay minerals used in poultry nutrition and both the antioxidant capacity and the bone biomechanical properties. In our study, the effect of sodium bentonite clay mineral on growth performance, blood antioxidant status and bone biomechanical properties of quails (Coturnix coturnix japonica) was investigated. In the study, a total of 180 one-day-old quail chicks were divided into 3 groups: a control group (C) and 2 experimental groups. The chicks were fed a basal diet or the basal diet supplemented with sodium bentonite at doses of 1% (NB1) and 2% (NB2). At the end of the experiment, quails fed with sodium bentonite showed a quadratic response in FI (P=0.037) related to growth performance, while other parameters were not affected. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) values related to antioxidant status showed linear responses (P=0.033; P≤0.001; P=0.008 and P≤0.001, respectively), whereas other blood antioxidant parameters were not affected. Bone biomechanical properties of the tibia and femur were not affected. This study demonstrates that sodium bentonite improves antioxidant status in quail but has no significant effects on growth performance or bone biomechanical properties, suggesting that this clay mineral may be a safe feed additive when used in a controlled manner.

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Cite This Article

Durna, Ö., Önk, K., Yıldız, G., Merhan, O., Elibol, F. K. E. (2026). Growth performance, blood antioxidant status, and bone biomechanical properties in Japanese quail (Coturnix coturnix japonica) fed diets supplemented with sodium bentonite. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Volume: 73 Issue: 3. https://doi.org/10.33988/auvfd.1706862

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References (60)

  1. Al‑Hammood AA, Frayyeh QJ, Abbas WA (2021): Raw bentonite as supplementary cementitious material – a review. J Phys Conf Ser, 1795, 012018. DOI: 10.1088/1742-6596/1795/1/012018
  2. AOAC (2000): Association of Official Methods of Analysis. 17th ed. AOAC Int.Gaithersburg, MD, USA.
  3. Avisar D, Primor O, Gozlan I, et al (2010): Sorption of sulfonamides and tetracyclines to montmorillonite clay. Water Air Soil Pollut, 209, 439‑450. DOI:10.1007/s11270-009-0212-8
  4. Aydemir B, Karadağ Sarı E (2009): Antioksidanlar ve büyüme faktörleri ile ilişkisi. Kocatepe Vet J, 2, 56‑60.
  5. Bailey CA, Latimer GW, Barr AC, et al (2006): Efficacy of montmorillonite clay (NovaSil PLUS) for protecting full‑term broilers from aflatoxicosis. J Appl Poult Res, 15,198‑206 DOI: 10.1093/japr/15.2.198
  6. Beutler E, Duron O, Kelly BM (1963): Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882‑888.
  7. Calik A, Yalçın S, Küçükersan S, et al (2019): Effects of calcium soaps of animal fats on performance, fatty acid composition and bone properties in broilers. Kafkas Univ Vet Fak Derg, 25, 61-70. DOI:10.9775/kvfd.2018.20329
  8. Cervini‑Silva J, Nieto‑Camacho A, Gómez‑Vidales V (2015): Oxidative stress inhibition and oxidant activity by fibrous clays. Colloids Surf B Biointerfaces, 133, 32‑35. DOI: 10.1016/j.colsurfb.2015.05.042
  9. Christidis G (1998): Physical and chemical properties of some bentonite deposits of Kimolos Island, Greece. Appl Clay Sci, 13, 79‑98. DOI: 10.1016/S0169-1317(98)00023-4
  10. Colombo JP, Richterich R (1964): Zur bestimmung des caeruloplasmin im plasma. Schweiz Med Wochenschr, 23,715‑720.
  11. Demirulus H, Aydın A (2012): Doğal zeolit (klinoptilolit)’in kanatlı hayvan beslemede kullanım olanakları. Turk Bilimsel Derlemeler Derg, 2, 147‑150.
  12. Doumas BT, Watson WA, Biggs HG (1971): Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta, 31, 87‑96. DOI: 10.1016/0009-8981(71)90365-2
  13. Durna Ö, Yıldız G, Güntürkün OB, et al (2020): The use of glauconite as a feed additive in broiler nutrition and its effect on growth performance, intestinal histomorphology and bone biomechanical properties. Kafkas Univ Vet Fak Derg, 26, 343‑349. DOI: 10.9775/kvfd.2019.23154
  14. Durna Ö, Merhan O, Yıldız G (2020): The effect of sodium bentonite on growth performance and some blood parameters in post‑weaning Tuj breed lambs. Ankara Univ Vet Fak Derg, 67, 235‑241. DOI: 10.33988/auvfd.590696
  15. Dündar Y, Aslan R (1999): Hücre moleküler statüsünün anlaşılması ve fizyolojik önem açısından radikaller ve antioksidanlar. Insizyon Cerrahi Tıp Bil Derg, 2, 134‑142
  16. EFSA (2011): Scientific Opinion on the safety and efficacy of bentonite (dioctahedral montmorillonite) as feed additive for all species. EFSA J, 9, 2007.
  17. EFSA (2017): Safety and efficacy of bentonite as a feed additive for all animal species. EFSA J, 15, e05096.
  18. Eng KS, Hutcheson DP, Bechtel R (2003): Adding potassium, clinoptilolite zeolite and yucca extract to feedlot diets to reduce nitrogen losses from manure. J Anim Sci, 81,15‑25.
  19. Ghazalah AA, Abd‑Elsamee MO, Moustafa KEM, et al (2021): Effect of Nanosilica and bentonite as mycotoxin adsorbents in broilers. Animals, 11, 2129. DOI: 10.3390/ani11072129
  20. Guo H, Miao YT, Xian JA, et al (2015): Expression profile of antioxidant enzymes in hemocytes from freshwater prawn Macrobrachium rosenbergii exposed to an elevated level of copper. Bull Environ Contam Toxicol, 95, 447‑451. DOI: 10.1007/s00128-015-1618-1
  21. Gurgul S, Erdal N, Yilmaz SN, et al (2008): Bone quality deterioration by long‑term exposure to extremely low frequency magnetic field. Bone, 42, 74‑80. DOI: 10.1016/j.bone.2007.08.040
  22. Gümüş E (2023): Dietary sodium bentonite supplementation in Japanese quails. Acta Vet Brno, 97, 197‑204. DOI:10.2754/avb202392020197
  23. Halici M, Imik H, Koc M, et al (2012): Effects of alpha‑lipoic acid and vitamins on performance and oxidative stress under heat stress in quails. Br Poult Sci, 53, 800-808. DOI: 10.1080/00071668.2012.740615
  24. Hernández D, Montalvo A, Pérez I, et al (2023): Bentonite/vitamin C hybrid: antioxidant efficacy and characterization. Pharmaceutics, 15, 1171. DOI:10.3390/ pharmaceutics15041171
  25. Khusid SB, Volkova SA, Yap D (2015): Feed additive based on bentonite and rice waste. Young Sci, 1, 135‑138.
  26. Kieronczyk B, Rawski M, Józefiak D, et al (2017): Leg disorders in poultry – a review. Ann Anim Sci, 17, 645‑669. DOI: 10.1515/aoas-2016-0098
  27. Kim GJ, Kim D, Lee KJ, et al (2020): Effects of nano‑montmorillonite on bone formation and mineralization. Nanomaterials, 10, 230. DOI: 10.3390/nano10020230
  28. Mahesh A, Lohan OP (2008): Effect of various binders on the quality of feed pellets and growth performance of broilers. Indian J Anim Nutr, 25, 182‑187.
  29. Mashkoor J, Al‑Saeed FA, Guangbin Z, et al (2023): Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents. Front Vet Sci, 10, 1128522. DOI:10.3389/fvets.2023.1128522
  30. Montayeva NS, Montayev SA, Montayeva AS (2023): Studies of Montmorillonitic (Bentonite) clay of Western Kazakhstan as a therapeutic mineral feed additive for animals and poultry. Agric Res, 12, 226‑231. DOI:10.1007/s40003-022-00634-7
  31. Morshdy AEM, Abdel Samie AA, Elshorbagy IM, et al (2021): Effect of bentonite, sepiolite, Coriobacteriaceae and fumonisesterase on growth rate, blood parameters, chicken meat quality and aflatoxin residues. Slov Vet Res, 58, 91-100. DOI: 10.26873/SVR-1430-2021
  32. Mumpton, F. A (1999): La Roca Magica: Uses of Natural Zeolites in Agriculture and Industry. Proceedings of the National Academy of Sciences of the United States of America, 96, 3463-3470.
  33. NRC (1994): Nutrient Requirements of Poultry. Natl. Acad. Press, Washington, DC, US.
  34. Onbaşılar EE, Yalçın S, Ateş K, et al (2025): Effects of zinc amino acid complex supplementation on welfare, carcass traits, tibia health, meat quality and myopathies in broilers. Vet Med Sci, 11, e70451. DOI: 10.1002/vms3. 70451
  35. Ogwuegbu MC, Udeh FU, Ugwuozor JC, et al (2025): Improving the physiological status of broiler chickens using rosemary leaf meal and sodium bentonite as ammonia gas control agents. J Appl Anim Res, 53, 2443185. DOI:10.1080/09712119.2024.2443185
  36. Onk K, Durna Ö, Yıldız G, et al (2024): Effect of chia oil addition to quail diets on growth performance, antioxidant status, SCFA and bone biomechanics. S Afr J Anim Sci, 54, 176‑185.
  37. Ouachem D, Meredef A, Kaboul N (2015): The marl and kaolin in broiler diet: Effects on bone weight and cutting yield. The 6 st Int Semin Trop Anim Prod, 2015, 72‑75.
  38. Ouhida I, Perez JF, Gasa J, et al (2000): Enzymes (β‑glucanase and arabinoxylanase) and/or sepiolite supplementation and the nutritive value of maize‑barley‑wheat diets for broilers. Br Poult Sci, 41, 617‑624. DOI: 10.1080/713654974
  39. Palanisamy GS, Kirk NM, Ackart DF, et al (2011): Evidence for oxidative stress and defective antioxidant response in guinea pigs with tuberculosis. PLoS One, 6, e26254.DOI: 10.1371/journal.pone.0026254
  40. Pappas AC, Zoidis E, Theophilou N, et al (2010): Effects of palygorskite on broiler performance, feed technological characteristics and litter quality. Appl Clay Sci, 49, 276‑280. DOI: 10.1016/j.clay.2010.06.003
  41. Ponnampalam EN, Kiani A, Santhiravel S, et al (2022): The importance of dietary antioxidants on oxidative stress, meat and milk production in farm animals. Animals, 12, 3279.DOI: 10.3390/ani12233279
  42. Qu XY, Chen JF, He CQ, et al (2018): Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status and immune response of laying hens. Livest Sci, 210, 15‑20. DOI: 10.1016/j.livsci.2018.01.021
  43. Safaei Katouli M, Boldaji F, Dastar B, et al (2012): Growth response and tibia bone characteristics in broilers fed diets containing kaolin, bentonite and zeolite. J Anim Feed Sci, 21, 334‑344.DOI:10.22358/jafs/66085/2012
  44. Sen S, Chakraborty R, Sridhar C, et al (2010): Free radicals, antioxidants, diseases and phytomedicines. Int J Pharm Sci Rev Res, 3, 91‑100.
  45. Sen S, Chakraborty R (2011): The role of antioxidants in human health. ACS Symp Ser, 1083, 1‑37.
  46. Skic A, Kołodziej P, Stropek Z, et al (2024): Analysis of mechanical properties of femurs and eggshells of Japanese quail lines. Adv Sci Technol Res J, 18, 437-446. DOI: 10.12913/22998624/193625
  47. SPSS (2011): Statistical Package for the Social Sciences, 20th ed. IBM Inc., Chicago, USA.
  48. Stojkovic J, Ilic Z, Ciric S, et al (2012): Efficiency of zeolite basis preparation in fattening lambs diet. Biotechnol Anim Husb, 28, 545‑552. DOI: 10.2298/BAH1203545S
  49. Suzer B, Petek M, Tufekci K, et al (2020): Comparison of biomechanical properties of tibiotarsus in four quail lines. Braz J Poult Sci, 22, eRBCA‑2019. DOI: 10.1590/1806-9061-2019-1204
  50. Turner CH, Burr DB (1993): Basic biomechanical measurements of bone. Bone, 14,595‑608.
  51. Tomaszewska E, Knaga S, Dobrowolski P, et al (2020): Effect of bee pollen on bone biomechanical strength and histomorphometry in quail. PLoS One, 15, e0230240.DOI: /10.1371/journal.pone.0230240
  52. Wang J, Hu J, Zhang S (2010): Studies on the sorption of tetracycline onto clays and marine sediment from seawater. J Colloid Interface Sci, 349, 578‑582. DOI: 10.1016/j.jcis.2010.04.081
  53. Wu QJ, Zhou YM, Wu YN, et al (2013): The effects of natural and modified clinoptilolite on intestinal barrier function and immune response to LPS in broilers. Vet Immunopathol, 153, 70‑76. DOI: 10.1016/j.vetimm.2013. 02.006
  54. Xia MS, Hu CH, Xu ZR (2004): Effects of copper‑bearing montmorillonite on growth performance, digestive enzyme activities and intestinal morphology in broilers. Poult Sci, 83, 1868‑1875. DOI: 10.1093/ps/83.11.1868
  55. Yalçın S, Eser H, Onbaşılar I, et al (2016): Effects of dietary sepiolite on performance, egg quality and some blood parameters in laying hens. Ankara Univ Vet Fak Derg, 63, 25‑29. DOI: 10.1501/Vetfak_0000002705
  56. Yalçın S, Gebeş ES, Şahin A, et al (2017): Sepiolite as a feed supplement for broilers. Appl Clay Sci, 148, 95‑102. DOI: 10.1016/j.clay.2017.08.007
  57. Yalçınkaya H, Yalçın S, Ramay MS, et al (2024): Evaluation of Spirulina platensis as a feed additive in low‑protein broiler diets. Int J Mol Sci, 26, 24. DOI: 10.3390/ijms26010024
  58. Yazar E, Er A, Uney K, et al (2010): Effects of drugs used in endotoxic shock on oxidative stress and organ damage markers. Free Radic Res, 44, 397‑402. DOI:10.3109/10715760903513025
  59. Yoshioka T, Kawada K, Shimada T, et al (1979): Lipid peroxidation in maternal and cord blood and protective mechanisms against oxygen toxicity. Am J Obstet Gynecol, 135, 372‑376.
  60. Calik A, Yalçın S, Küçükersan S, et al (2019): Effects of calcium soaps of animal fats on performance, fatty acid composition and bone properties in broilers. Kafkas Univ Vet Fak Derg, 25, 61-70. DOI:10.9775/kvfd.2018.20329