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Journals / Animal Health Production and Hygiene / 2026 / Cilt: 15 Sayı: 1

Effects of Acute Heat Stress Duration on Hepatic HSP70, GR, and AMPKα Gene Expression in Texas Quail

Teksas Bıldırcınlarında Akut Isı Stresi Süresinin Karaciğer HSP70, GR ve AMPKα Gen Ekspresyonuna Etkisi

Gonca Sönmez(SELCUK UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF ZOOTECHNICS AND ANIMAL FEEDING, DEPARTMENT OF GENETICS)0000-0002-4946-3749
Emre Arslan(SELCUK UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF ZOOTECHNICS AND ANIMAL FEEDING, DEPARTMENT OF ZOOTECHNICS)0000-0002-4609-8395
Muhammed Hüdai Çulha(SELCUK UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF ZOOTECHNICS AND ANIMAL FEEDING, DEPARTMENT OF GENETICS)0000-0002-8963-5605
Mehmet Aydoğdu(SELCUK UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF ZOOTECHNICS AND ANIMAL FEEDING, DEPARTMENT OF ZOOTECHNICS)0009-0002-9917-1311
Pages
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Publication Date
2026-03-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, it was shown that the liver tissue of Texas quail develops a significant molecular response to acute heat stress.

📌 3 Key Takeaways

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Objective & Hypothesis:Heat stress is a significant environmental factor in poultry production, negatively impacting growth performance, egg yield, and mortality rates.
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Key Findings & Data:The present work examines how short- and long-term thermal exposure modulates liver expression patterns of heat shock protein 70 (HSP70), glucocorticoid receptor (GR), and AMP-activated protein kinase alpha (AMPKα) genes in the liver tissue of 6-week-old male Texas quail (Coturnix japonica).
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Conclusion & Impact:In conclusion, it was shown that the liver tissue of Texas quail develops a significant molecular response to acute heat stress.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Gen regülasyonu · Isı şok proteini 70 · Japon bıldırcını

Abstract

Heat stress is a significant environmental factor in poultry production, negatively impacting growth performance, egg yield, and mortality rates. The present work examines how short- and long-term thermal exposure modulates liver expression patterns of heat shock protein 70 (HSP70), glucocorticoid receptor (GR), and AMP-activated protein kinase alpha (AMPKα) genes in the liver tissue of 6-week-old male Texas quail (Coturnix japonica). A total of 24 quail randomly assigned into three experimental groups: a thermoneutral control group (24 ± 1 °C), a group maintained under heat-stress conditions for an 8 hour duration (34 ± 1 °C), and maintained under heat-stress conditions for a 24 hours (34 ± 1 °C). Liver tissues were collected after heat exposure, and cDNA synthesis was performed following total RNA isolation. SYBR Green-based qRT-PCR was employed to quantify gene expression levels. o compare the experimental groups, a one-way ANOVA was used, and pairwise comparisons were conducted with the Tukey test. Compared with the control group, both heat-stressed groups exhibited a significant elevation in HSP70 expression (p < 0.001). GR expression was similar in the control group and the 8-hour heat stress group, while it was showed a significantly higher level in the 24-hour heat stress group (p = 0.003). AMPKα expression was similarly higher in both heat stress groups. In conclusion, it was shown that the liver tissue of Texas quail develops a significant molecular response to acute heat stress. The expression profiles of HSP70, GR, and AMPKα genes suggest that they can be used as potential molecular biomarkers in the assessment of heat stress in poultry.

Özet

Isı stresi, kümes hayvanı üretiminde önemli bir çevresel faktördür ve büyüme performansını, yumurta verimini ve ölüm oranlarını olumsuz etkiler. Bu çalışma, kısa ve uzun süreli ısıya maruz kalmanın, 6 haftalık erkek Teksas bıldırcınlarının (Coturnix japonica) karaciğer dokusunda ısı şok proteini 70 (HSP70), glukokortikoid reseptörü (GR) ve AMP-aktive protein kinaz alfa (AMPKα) genlerinin ekspresyon paternlerini nasıl modüle ettiğini incelemektedir. Toplam 24 bıldırcın rastgele üç deney grubuna ayrılmıştır: termonötr kontrol grubu (24 ± 1 °C), 8 saat süreyle ısı stresi koşullarında tutulan grup (34 ± 1 °C) ve 24 saat süreyle ısı stresi koşullarında tutulan grup (34 ± 1 °C). Isıya maruz kalmanın ardından karaciğer dokuları toplanmış ve toplam RNA izolasyonunun ardından cDNA sentezi yapılmıştır. Gen ekspresyon seviyelerini ölçmek için SYBR Green bazlı qRT-PCR kullanılmıştır. Deneysel grupları karşılaştırmak için tek yönlü ANOVA kullanıldı ve ikili karşılaştırmalar Tukey testi ile yapıldı. Kontrol grubuyla karşılaştırıldığında, her iki ısı stresi grubunda da HSP70 ekspresyonunda anlamlı bir artış gözlendi (p < 0.001). GR ekspresyonu kontrol grubu ve 8 saatlik ısı stresi grubunda benzerdi, ancak 24 saatlik ısı stresi grubunda anlamlı derecede daha yüksek bir seviye gösterdi (p = 0.003). AMPKα ekspresyonu her iki ısı stresi grubunda da benzer şekilde daha yüksekti. Sonuç olarak, Teksas bıldırcınlarının karaciğer dokusunun akut ısı stresine karşı önemli bir moleküler yanıt geliştirdiği gösterilmiştir. HSP70, GR ve AMPKα genlerinin ekspresyon profilleri, bunların kümes hayvanlarında ısı stresinin değerlendirilmesinde potansiyel moleküler biyobelirteçler olarak kullanılabileceğini düşündürmektedir.

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Cite This Article

Sönmez, G., Arslan, E., Çulha, M. H., Aydoğdu, M. (2026). Effects of Acute Heat Stress Duration on Hepatic HSP70, GR, and AMPKα Gene Expression in Texas Quail. Animal Health Production and Hygiene, Cilt: 15 Sayı: 1. https://doi.org/10.53913/aduveterinary.1859341

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