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Journals / Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi / 2024 / Cilt: 21 Sayı: 2

DNA Barcoding of Docotettix cornutus Ribaut, 1948 (Hemiptera: Cicadellidae)

Docotettix cornutus Ribaut, 1948 (Hemiptera:Cicadellidae)’un DNA Barkodlaması

Sema Avtaş(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ, TARIMSAL BİYOTEKNOLOJİ PR.)0000-0001-5673-700X
Eyyüp Mennan Yıldırım(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ, TARIMSAL BİYOTEKNOLOJİ PR.)0000-0002-0580-0555
Pages
173–176
Publication Date
2024-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When the specimens were compared with other registered specimens in the BLAST module at NCBI, it was seen that the closest species to D. cornutus was Synophropsis lauri.

📌 3 Key Takeaways

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Objective & Hypothesis:Docotettix cornutus Ribaut 1948 (Hemiptera: Cicadellidae) is a species that can cause economic damage to crops such as tomato, olive, pomegranate, and maize and is distributed worldwide.
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Key Findings & Data:After morphological identification of the species, HCO/ LCO universal primers were used to amplify the COI gene region of the specimen, and healthy reads were made between 499 bp (SE05) and 675 bp (SE07).
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Conclusion & Impact:When the specimens were compared with other registered specimens in the BLAST module at NCBI, it was seen that the closest species to D. cornutus was Synophropsis lauri.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:COI · DNA barkodlama · Docotettix cornutus

Abstract

Docotettix cornutus Ribaut 1948 (Hemiptera: Cicadellidae) is a species that can cause economic damage to crops such as tomato, olive, pomegranate, and maize and is distributed worldwide. Since morphological identification is very difficult due to the small size of this pest there is a lack of experts and no record of molecular identification, DNA barcoding was aimed. In this study, D. cornutus was collected from pomegranate orchards in Aydın, İzmir and Manisa provinces (Türkiye) using Japanese umbrellas and suction tubes. After morphological identification of the species, HCO/ LCO universal primers were used to amplify the COI gene region of the specimen, and healthy reads were made between 499 bp (SE05) and 675 bp (SE07). Sequence analysis of the specimen was performed by Triogen Biotechnology (Istanbul-Türkiye). D. cornutus was registered in Genebank for the first time and a GeneBank accession number was obtained. When the specimens were compared with other registered specimens in the BLAST module at NCBI, it was seen that the closest species to D. cornutus was Synophropsis lauri.

Özet

Docotettix cornutus Ribaut 1948 (Hemiptera: Cicadellidae) domates, zeytin, nar ve mısır gibi bitkiler üzerinde ekonomik zarara neden olabilen ve tüm dünyada yayılış gösteren bir türdür. Morfolojik teşhisi zararlının küçük olması ve uzman eksikliği gibi nedenlerle oldukça zor yapılabilmekte olup, moleküler teşhisi ile ilgili herhangi bir kayıt da bulunmaması nedeniyle DNA barkodlamasının yapılması amaçlanmıştır. Çalışmada D. cornutus; Aydın, İzmir ve Manisa illerinde (Türkiye) nar bahçelerinden japon şemsiyesi ve emgi tüpü yardımıyla toplanmıştır. Morfolojik olarak tür teşhisi yapıldıktan sonra; örneklerin COI gen bölgesinin çoğaltılması için HCO/ LCO evrensel primerler kullanılmış ve 499bç (SE05) ile 675bç (SE07) arasında sağlıklı okuma yapılmıştır. Örneklerin sekans analizi Triogen Biyoteknoloji (İstanbul-Türkiye) tarafından yapılmıştır. D. cornutus ilk kez Genbank’a kaydedilmiş ve Gen Bankası erişim numarası alınmıştır. Örnekler; NCBI’da BLAST modülünde diğer kayıtlı örneklerle karşılaştırıldığında D. cornutus ’a en yakın türün Synophropsis lauri olduğu görülmüştür.

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Cite This Article

Avtaş, S., Yıldırım, E. M. (2024). DNA Barcoding of Docotettix cornutus Ribaut, 1948 (Hemiptera: Cicadellidae). Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 21 Sayı: 2, 173–176. https://doi.org/10.25308/aduziraat.1487702

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