SavedAdd your journal

Journals / Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi / 2024 / Cilt: 21 Sayı: 2

The detection of RT-qPCR method of Tospoviruses carried by Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae)

Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae) tarafından taşınan Tospovirüslerin Moleküler Tespitinde RT-qPCR yönteminin uygulanması

Murat Kemal Avcı(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ, ENZİM VE MİKROBİYEL BİYOTEKNOLOJİ ANABİLİM DALI)0000-0001-5211-2352
Ferhat Kiremit(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ, HAYVANSAL BİYOTEKNOLOJİ ANABİLİM DALI)0000-0003-4729-7676
Eyyüp Mennan Yıldırım(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ, TARIMSAL BİYOTEKNOLOJİ PR.)0000-0002-0580-0555
Pages
187–193
Publication Date
2024-12-31
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Both methods seem promising to be used in future studies in terms of providing fast and reliable results.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) is a polyphagous species and can cause damage to many agricultural products such as cotton, strawberries, tomato and ornamental plants.
📊
Key Findings & Data:F. occidentalis sucks the sap from the flowers of plants and causes premature flower fall and low yield.
💡
Conclusion & Impact:Both methods seem promising to be used in future studies in terms of providing fast and reliable results.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Frankliniella occidentalis · RFLP · Tospovirüs

Abstract

Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) is a polyphagous species and can cause damage to many agricultural products such as cotton, strawberries, tomato and ornamental plants. F. occidentalis sucks the sap from the flowers of plants and causes premature flower fall and low yield. In addition, it can cause indirect damage because it is a virus vector. In this study, it was aimed to perform molecular identification of thrips obtained from the flowers of strawberry plants, which have economic importance for Aydın province, by applying PCR-RFLP protocol and to detect the presence of tospoviruses by the RT-qPCR method by obtaining RNA isolates. As a result of the study; it was determined that all species obtained were F. occidentalis. In addition, the presence of tospoviruses transmitted by these insects was also detected. Both methods seem promising to be used in future studies in terms of providing fast and reliable results.

Özet

Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) polifag bir tür olup, pamuk, çilek, domates ve süs bitkileri gibi birçok tarımsal üründe zararlara yol açabilmektedir. F. occidentalis bitkilerin çiçeklerindeki özsuyunu emmek suretiyle çiçeklerin erken dökülmesine ve verim düşüklüğüne neden olmaktadır. Ayrıca virüs vektörü olması nedeniyle bitkilerde dolaylı yoldan da zarar yapabilmektedir. Bu çalışmanın amacı, Aydın ili için ekonomik öneme sahip çileklerden elde edilen tripslerde vektörü oldukları tospovirüslerin varlığının RT-qPCR yöntemiyle tespit edilmesidir. Bu amaçla ilk olarak çilek bitkilerinin çiçeklerinden elde edilen tripslerin PCR-RFLP protokolüyle moleküler tanılaması yapıldıktan sonra RNA izolatları elde edilerek RT-qPCR yöntemiyle tospovirüslerin varlığı tespit edilmiştir. Çalışma sonucunda; elde edilen verilerle, eldeki örneklerin F. occidentalis olduğu ve bu böceklerle taşınan virüslerin de tospovirüs olduğu tespit edilmiştir. Her iki yöntem (PCR-RFLP ve RT-qPCR) hızlı ve güvenilir sonuç vermesi açısından, hem trips tespiti hem de tospovirüs tespiti amacıyla önümüzdeki çalışmalarda kullanılmak için ümitvar görünmektedir.

🔖

Cite This Article

Avcı, M. K., Kiremit, F., Yıldırım, E. M. (2024). The detection of RT-qPCR method of Tospoviruses carried by Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae). Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 21 Sayı: 2, 187–193. https://doi.org/10.25308/aduziraat.1523822

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (26)

  1. Beaudoin ALP, Kahn ND, Kennedy GG (2009) Bell and Banana Pepper Exhibit Mature-Plant resistance to Tomato Spotted Wilt Tospovirus Transmitted by Frankliniella fusca (Thysanoptera: Thripidae). Journal of Economic Entomology 102 (1): 30-35.
  2. Brunner P, Fleming C, Frey J (2002) A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLPbased approach. Agricultural and Forest Entomology 4 (2): 127–136.
  3. Coll M, Shakya S, Shouster I, Nenner Y, Steinberg S (2006) Decision-making tools for Frankliniella occidentalis management in strawberry: consideration of target markets. Entomologia Experimentalis et Applicata, 122 (1): 59-67.
  4. Doğanlar M, Aydın S (2009) Güneydoğu Anadolu Bölgesi (Türkiye)’nde yeni bir zararlı, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Türkiye Entomoloji Dergisi, 33(2): 153-160.
  5. Eiras M, Resende RO, Missiaggia AA, Avila AC (2001) RT-PCR and dot blot hybridization methods for a universal detection of tospoviruses. Fitopatologia Brasileira, 26:170–175. https://doi.org/10.1590/s0100-41582001000200009
  6. Huang, KS., Lee, SE, Yeh Y, Shen GS, Mei E, Chan CM (2010) Taqman real-time quantitative PCR for identification of western flower thrip (Frankliniella occidentalis) for plant quarantine. Biology letters, 6(4):555-557.
  7. Kirk WDJ, Terry LI (2003) The spread of the western flower thrips Frankliniella occidentalis (Pergande). Agricultural and Forest Entomology,5 (4): 301-310.
  8. Kuipers AGJ, Jongsma MA (2004) Isolation and molecular characterization of cathepsin L-like cysteine protease cDNAs from western flower thrips (Franhkliniella occidentalis). Comparative Biochemistry and Physiology, Part B, 139 (1): 65-75.
  9. Law MD, Speck J, MOYER JW (1992) The M RNA of lmpatiens Necrotic Spot Tospovirus (Bunyaviridae) Has an Ambisense Genomic Organization. Virology, 188 (2):732-741.
  10. Li HB, Du YZ (2013) Molecular cloning and characterization of an Hsp90/70 organizing protein gene from Frankliniella occidentalis (Insecta: Thysanoptera, Thripidae). Gene, 520 (2): 148–155.
  11. Mainali BP, Shrestha S, Lim UT, Kim Y (2008) Molecular markers of two sympatric species of the genus Frankliniella (Thysanoptera: Thripidae). Journal of Asia-Pasific Entomology 11(1): 45-48.
  12. Marie-Nguyen M, Haenni AL (2003) Expression strategies of ambisense viruses. Virus Research, 93 (2): 141-150.
  13. Moriones E, Aramburu J, Riudavets J, Arno J, Lavina A (1998). Effect of plant age at time of infection by tomato spotted wilt tospovirus on the yield of field-grown tomato. European Journal of Plant Pathology 104: 295-300.
  14. Nagata T, Almeida ACL, Resende RO, DeAvila AC (2004) The competence of four thrips species to transmit and replicate four tospoviruses. Plant Pathology 53(2): 136–140.
  15. Navas SVE, Funderburk JE, Beshear RJ, Olson SM, Mack TP (1991) Seasonal Patterns of Frankliniella spp. (Thysanoptera: Thripidae) in Tomato Flowers. Journal of Economic Entomology,84 (6):1818-1822.
  16. Okazaki S, Okuda M, Komi K, Yamasaki S, Okuda S, Sakurai T, Iwanami T (2011) The effect of virus tirtre on acquisition efficiency of Tomato spotted wilt virus by Frankliniella occidentalis and the effect of temperature on detectable period of the virus in dead bodies. Australian Plant Pathology, 40: 120-125.
  17. Okuda M, Hanada K (2001) RT-PCR for detecting five distinct Tospovirus species using degenerate primers and dsRNA template. Journal of Virological Methods, 96 (2): 149–156. Riley DG, Joseph S, Srinivasan S, Diffie S (2011) Thrips Vectors of Tospoviruses. Journal of Interated. Pest Management, 2(1): I1-I10.
  18. Roberts CA, Dietzgen RG, Heelan LA, Maclean DJ (2000) Real-time RT-PCR fluorescent detection of tomato spotted wilt virüs. Journal of Virological Methods, 88 (1): 1–8.
  19. Rotenberg D, Kumar NKK, Ullman DE, Astua MM, Willis DK, German TL, Whitfield AE (2009) Variation in Tomato spotted wilt virus Titer in Frankliniella occidentalis and Its Association with frequency of Transmission, Phytopathology 99(4): 404-410.
  20. Schoonhoven AV, Peña JE (1976) Estimation of Yield Losses in Cassava Following Attack from Thrips. Journal of Economic Entomology, 69 (4):514-516.
  21. Sokmen MA, Sevik MA (2013) Spread of Tomato spotted wit virus from an internal virus source by thrips species in Samsun, Turkey. Phytoparasitica, 41: 159-168.
  22. Toda S, Komazaki S (2002) Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. Bulletin of Entomological Research 92 (4): 359–363.
  23. Tunç I, Göçmen H (1994) New greenhouse pests, Polyphagotarsonemus latus and Frankliniella occidentalis in Turkey. FAO Plant Protection Bulletin, 42: 218–220.
  24. Wijkamp I, Van Lent J, Kormelink R, Goldbach R, Peters D (1993) Multiplication of tomato spotted wilt virus in its insect vector, Franklinieila occidentalis. Journal of General Virology,74 (3):341-349.
  25. Yıldırım EM, Başpınar H (2013) Aydın ili çilek alanlarında Frankliniella occidentalis (Pergende) (Thysanoptera: Thripidae)’nin popülasyon dalgalanmaları. Türkiye Entomoloji Bülteni, 3(4): 135-138.
  26. Zhang GF, Meng XQ, Min L, Qiao WN, Wan FH (2012) Rapid diagnosis of the invasive species, Frankliniella occidentalis (Pergande): a species-specific COI marker, Journal of Applied Entomology, 136 (6): 410–420