SavedAdd your journal

Journals / Meyve Bilimi / 2026 / Cilt: 13 Sayı: 1

Effect of Different Auxins on Root and Vegetative Development of Aronia melanocarpa cv. Nero Under In Vitro Conditions

In Vitro Koşullarda Aronia melanocarpa cv. Nero’da Farklı Oksinlerin Kök ve Vejetatif Gelişime Etkisi

Heydem Ekinci(Harran Üniversitesi)0000-0002-1828-7367
Zahide Polat0009-0009-0803-2617
Necla Şaşkın(DICLE UNIVERSITY)0000-0003-3828-0522
Bekir Erol Ak(HARRAN UNIVERSITY)0000-0001-6938-942X
Zehra Günbeği0009-0009-5073-441X
Pages
80–87
Publication Date
2026-07-20
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, the highest plant height was observed in the 2 mg L-1 IBA + 2 mg L-1 NAA application, and the highest number of leaves was observed in the 2 mg L-1 NAA and 1 mg L-1 NAA + 1 mg L-1 IBA applications.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aronia is a berry-like fruit with high antioxidant content and can be propagated by generative and vegetative methods.
📊
Key Findings & Data:Among vegetative propagation methods, plant tissue culture techniques are an important method due to their high propagation rate and the ability to produce disease and pest-free plant material.
💡
Conclusion & Impact:According to the results, the highest plant height was observed in the 2 mg L-1 IBA + 2 mg L-1 NAA application, and the highest number of leaves was observed in the 2 mg L-1 NAA and 1 mg L-1 NAA + 1 mg L-1 IBA applications.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Köklenme · IBA · mikroçoğaltım

Abstract

Aronia is a berry-like fruit with high antioxidant content and can be propagated by generative and vegetative methods. Among vegetative propagation methods, plant tissue culture techniques are an important method due to their high propagation rate and the ability to produce disease and pest-free plant material. This study was conducted to determine the effects of different auxin types and concentrations used in the in vitro rooting stage of aronia plantlets on rooting and vegetative development. IBA and NAA at concentrations of 1 and 2 mg L-1, and combinations of these concentrations, were applied to the culture medium during the rooting stage. Morphological parameters such as plant height, number of leaves, leaf area, rooting rate, root length, and number of roots were examined in the study. According to the results, the highest plant height was observed in the 2 mg L-1 IBA + 2 mg L-1 NAA application, and the highest number of leaves was observed in the 2 mg L-1 NAA and 1 mg L-1 NAA + 1 mg L-1 IBA applications. The highest rooting rate and number of roots were obtained with the 1 mg L-1 NAA + 1 mg L-1 IBA application, while the longest root was obtained with the 1 mg L-1 IBA application. In conclusion, it was determined that combinations of IBA and NAA are effective in increasing rooting success.

Özet

Aronya, yüksek antioksidan içeriğine sahip üzümsü bir meyve olup generatif ve vejetatif yöntemlerle çoğaltılabilmektedir. Vejetatif çoğaltma yöntemleri arasında yer alan bitki doku kültürü teknikleri, yüksek çoğaltma oranı sağlaması ve hastalık ile zararlılardan ari bitkisel materyal üretimine olanak tanıması açısından önemli bir yöntemdir. Bu çalışma, aronya bitkiciklerinin in vitro köklenme aşamasında kullanılan farklı oksin tip ve konsantrasyonlarının köklenme ve vejetatif gelişim üzerine etkilerini belirlemek amacıyla yürütülmüştür. Köklenme aşamasında besi ortamına 1 ve 2 mg L-1 konsantrasyonlarında IBA ve NAA ile bu konsantrasyonların kombinasyonları uygulanmıştır. Araştırmada bitki boyu, yaprak sayısı, yaprak alanı, köklenme oranı, kök uzunluğu ve kök sayısı gibi morfolojik parametreler incelenmiştir. Sonuçlara göre en yüksek bitki boyu 2 mg L-1 IBA + 2 mg L-1 NAA uygulamasında, en fazla yaprak sayısı 2 mg L-1 NAA ile 1 mg L-1 NAA + 1 mg L-1 IBA uygulamalarında belirlenmiştir. En yüksek köklenme oranı ve kök sayısı 1 mg L-1 NAA + 1 mg L-1 IBA uygulamasında, en uzun kök ise 1 mg L-1 IBA uygulamasında elde edilmiştir. Sonuç olarak, IBA ve NAA kombinasyonlarının köklenme başarısını artırmada etkili olduğu belirlenmiştir.

🔖

Cite This Article

Ekinci, H., Polat, Z., Şaşkın, N., Ak, B. E., Günbeği, Z. (2026). Effect of Different Auxins on Root and Vegetative Development of Aronia melanocarpa cv. Nero Under In Vitro Conditions. Meyve Bilimi, Cilt: 13 Sayı: 1, 80–87. https://doi.org/10.51532/meyve.1922109

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (42)

  1. Ak BE, Kıyar PK, Hatipoglu IH, Dikmetaş B, 2021. Effects of different BA and IBA concentrations on proliferation and rooting of ‘GARNEM’ rootstock in vitro propagation. International Journal of Agriculture Environment and Food Sciences 5(4): 470-476.
  2. Almokar HMM, Pırlak L, 2018. Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk Journal of Agriculture and Food Sciences 32(3): 549-558.
  3. Demirel F, Uğur R, Popescu GC, Demirel S, Popescu M, 2023. Usage of Machine learning algorithms for establishing an effective protocol for the in vitro micropropagation ability of black chokeberry (Aronia melanocarpa (Michx.) Elliott). Horticulturae 9(10): 1112.
  4. Denev P, Číž M, Kratchanova M, Blazheva D, 2019. Black chokeberry (Aronia melanocarpa) polyphenols reveal different antioxidant, antimicrobial and neutrophil-modulating activities. Food chemistry 284:108-117.
  5. Ekinci H, Dikmetaş Doğan B, Şaşkın N, Aydınlık Y, Ak BE, 2025. Effects of Healing Agents on Aronia Melanocarpa cv. ‘Nero’ Under In Vitro Conditions Against NaHCO3-Induced Alkaline Stress. Applied Fruit Science 67(2): 1-9.
  6. Ekinci H, Saskin N, Ak BE, Aydinlik Y, 2025a. Optimization of Micropropagation of ARDA® ([Prunus dulcis× Prunus persica] × Prunus amygdalus) Rootstock in In Vitro Conditions. Applied Fruit Science 67(1): 26.
  7. Ekinci H, Şaşkın N, Dikmetaş B, Ak BE, 2024a. Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions. International Journal of Agriculture Environment and Food Sciences 8(4): 760-767.
  8. Ekinci H, Saskin N, Korkmaz Ş, Aydınlık Y, 2024. The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 52(1): 13542-13542.
  9. Engin SP, Boz Y, Mert C, Fidanci A, İkinci A, 2018. Growing aronia berry (Aronia melanocarpa (Michx.) Elliot). In: In International Gap Agriculture & Livestock Congress. Sanliurfa (pp. 665-667).
  10. Estrella-Maldonado H, Chan-León A, Fuentes G, Girón-Ramírez A, Desjardins Y, Santamaría JM, 2022. The interaction between exogenous IBA with sucrose, light and ventilation alters the expression of ARFs and Aux/IAA genes in Carica papaya plantlets. Plant molecular biology 110(1): 107-130.
  11. Farah AK, Al-Eqabi FA, Al-Amery LKJ, 2025. Micropropagation of Prunus Sp., Factors, Hormones: A Review. In IOP Conference Series: Earth and Environmental Science 1487(1): 012070.
  12. George EF, Hall MA, Klerk GJD, 2008. Micropropagation: uses and methods. In: George EF, Hall MA, Klerk GJD (eds), In Plant Propagation by Tissue Culture. Dordrecht Springer Netherlands 29-64.
  13. Güney M, 2019. Development of an in vitro micropropagation protocol for Myrobalan 29C rootstock. Turkish Journal of Agriculture and Forestry 43(6): 569-575.
  14. Hermans C, Hammond JP, White PJ, Verbruggen N, 2006. How do plants respond to nutrient shortage by biomass allocation? Trends in plant science 11(12): 610-617.
  15. Hirvi T, Honkanen E, 1985. Analysis of the volatile constituents of black chokeberry (Aronia melanocarpa Ell.). Journal of the Science of Food and Agriculture 36(9): 808-810.
  16. Jabeen F, Arshad M, Qayyum MMN, Zaman MS, Shafique I, 2021. Exploring the effects of indole butyric acid (IBA) on in vitro growth of potato (Solanum tuberosum). Advances in Agriculture and Biology 4(1).
  17. Jakhar ML, Choudhary K, 2023. Effect of Plant Growth Regulators on in vitro Morphogenic Response of Gliricidia [Gliricidia sepium (Jacq.) Steud.]. Indian Journal of Agricultural Research 57(5).
  18. Kara Z, Yazar K, Ekinci H, Doğan O, Özer A, 2022. The effects of ortho silicone applications on the acclimatization process of grapevine rootstocks. Selcuk Journal of Agriculture and Food Sciences 36(2): 233-237.
  19. Kumlay AM, 2014. Combination of the auxins NAA, IBA, and IAA with GA3 improves the commercial seed‐tuber production of Potato (Solanum tuberosum L.) under in vitro conditions. BioMed research international 2014(1): 439259.
  20. Lakho MA, Jatoi MA, Solangi N, Abul-Soad AA, Qazi MA, Abdi G, 2023. Optimizing in vitro nutrient and ex vitro soil mediums-driven responses for multiplication, rooting, and acclimatization of pineapple. Scientific Reports 13(1): 1275.
  21. Lawson JD, Bridges WC, Adelberg JW, 2023. IBA delivery technique and media salts affected in vitro rooting and acclimatization of eight Prunus genotypes. Plants 12(2): 289.
  22. Litwińczuk W, 2012. Micropropagation of chokeberry by in vitro axillary shoot proliferation. In Protocols for micropropagation of selected Economically-Important horticultural plants. Springer, Berlin Heidelberg, 179-186.
  23. Mansseri-Lamrioui A, Louerguioui A, Bonaly J, Yakoub-Bougdal S, Allili N, Gana-Kebbouche S, 2011. Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration. African Journal of Biotechnology 10(43): 8613-8624.
  24. Mohusien R, Musallam IW, Barriah H, Qutteneh N, Masry SA, 2025. Improving Pineapple (Ananas comosus L.) Micropropagation: Effects of Different Types and Levels of Auxin on Root Development. Indian Journal of Agricultural Research 59.
  25. Murashige T, Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum 15(3).
  26. Nagini AMS, Karthik K, 2025. Tissue culture and micropropagation of ornamentals. Floriculture and landscape architecture: Emerging trends and technologies 1-17.
  27. Nas Z, Eşitken A, Pirlak L, 2023. The responses of ‘Viking’ Aronia variety to salinity stress under in vitro conditions. Erwerbs-Obstbau 65(6): 2547-2552.
  28. Nazir U, Gul Z, Shah GM, Khan NI, 2022. Interaction effect of auxin and cytokinin on in vitro shoot regeneration and rooting of endangered medicinal plant Valeriana jatamansi Jones through tissue culture. American Journal of Plant Sciences 13(2): 223-240.
  29. Parvin MS, Haque ME, Akhter F, Moniruzzaman M, Khaldun ABM, 2009. Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid. Bangladesh Journal of Agricultural Research 34(3): 411-416.
  30. Polat M, Eskimez I, 2022. The effects of different hormone combinations on in vitro micropropagation of aronia (Aronia melanocarpa (Michx.) Elliott). Fresenius Environmental Bulletin 31(01A): 1219-1227.
  31. Poniewozik M, Parzymies M, Szot P, Rubinowska K, 2021. Paphiopedilum insigne morphological and physiological features during in vitro rooting and ex vitro acclimatization depending on the types of auxin and substrate. Plants 10(3): 582.
  32. Puig J, Pauluzzi G, Guiderdoni E, Gantet P, 2012. Regulation of shoot and root development through mutual signaling. Molecular plant 5(5): 974-983.
  33. Rahayu YS, Walch-Liu P, Neumann G, Römheld V, von Wirén N, Bangerth F, 2005. Root-derived cytokinins as long-distance signals for NO3−-induced stimulation of leaf growth. Journal of experimental botany 56(414): 1143-1152.
  34. Sakakibara H, Takei K, Hirose N, 2006. Interactions between nitrogen and cytokinin in the regulation of metabolism and development. Trends in plant science 11(9): 440-448.
  35. Saskin N, Ak BE, Ekinci H, 2022. The usage of node culture in vitro conditions. 5th International Agriculture Congress, 5–6 Dec 2022, 90–99, Denizli.
  36. Şaşkın N, Ak BE, Eki̇nci̇ H, Sarioğlu A, 2026. Effects of sodium nitroprusside and salicylic acid applications on morphological, physiological and biochemical properties of Garnem (Prunus dulcis× Prunus persica) rootstock against alkaline stress under in vitro conditions. BMC Plant Biology.
  37. Shukla MR, Piunno K, Saxena PK, Jones AMP, 2020. Improved in vitro rooting in liquid culture using a two piece scaffold system. Engineering in Life Sciences 20(3-4): 126-132.
  38. Şirin U, Kabakcı M, Di̇ndar C, 2025. Effects of Different Dose Combinations of 6-Benzyl Amino Purine and Naphthalene Acetic Acid on Shoot Regeneration and Some Morphological Characteristics of New Shoots in Micropropagation of Aronia melanocarpa (Michx) Elliott.‘Viking’. Applied Biochemistry and Biotechnology 1-14.
  39. Sivanesan I, Saini RK, Kim DH, 2016. Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (michx.) Elliott. Industrial Crops and Products 83:31-38.
  40. Yaman M, Palaz EB, Isak MA, Demirel S, İzgü T, Adalı S, Demirel F, Şimşek Ö, Popescu GC, Popescu M, 2025. Integrating in vitro propagation and machine learning modeling for efficient shoot and root development in Aronia melanocarpa. Horticulturae 11(8): 886.
  41. Yazar K, 2025. Evaluation of the effects of chitosan application and growing media on the adaptation process of fercal grape rootstock. BMC Plant Biology 25(1): 1152.
  42. Yılmaz A, Güler E, Soydemir HE, Demirel S, Mollahaliloğlu S, Çiftçi V, Karadeniz T, 2021. Miracle plant: Black chokeberry (Aronia melanocarpa). MAS Journal of Applied Sciences 6(1): 83-94.