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Journals / Meyve Bilimi / 2024 / Cilt: 11 Sayı: 2

In vitro Propagation Techniques for 'Ferragnes' and 'Ferraduel' Almond Cultivars: Concentration Optimization and Rooting Success

'Ferragnes' ve 'Ferraduel' Badem Çeşitlerinin İn Vitro Çoğaltma Tekniklerinin Geliştirilmesi: Konsantrasyon Optimizasyonu ve Köklenme Başarısı

Fatma Hammud0000-0002-7154-7543
Bekir Erol Ak(HARRAN ÜNİVERSİTESİ)0000-0001-6938-942X
Ibrahim Halil Hatipoglu(HARRAN ÜNİVERSİTESİ)0000-0002-7236-4976
Heydem Ekinci0000-0002-1828-7367
Birgül Dikmetaş(HARRAN ÜNİVERSİTESİ)0000-0003-3618-3307
Pages
79–85
Publication Date
2024-12-23
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

For the 'Ferragnes' variety, it was found that 1.0 mg L-1 BAP concentration was more suitable, resulting in a higher number of leaves.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, an appropriate in vitro propagation technique was determined for 'Ferragnes' and 'Ferraduel' almond varieties, which are used in almond orchards for various reasons.
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Key Findings & Data:To facilitate shoot and proliferation development in the culture medium, 1 mg L-1 and 2 mg L-1 concentrations of BAP were added to the MS medium.
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Conclusion & Impact:For the 'Ferragnes' variety, it was found that 1.0 mg L-1 BAP concentration was more suitable, resulting in a higher number of leaves.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Badem · mikroçoğaltım · doku kültürü

Abstract

In this study, an appropriate in vitro propagation technique was determined for 'Ferragnes' and 'Ferraduel' almond varieties, which are used in almond orchards for various reasons. To facilitate shoot and proliferation development in the culture medium, 1 mg L-1 and 2 mg L-1 concentrations of BAP were added to the MS medium. For the 'Ferragnes' variety, it was found that 1.0 mg L-1 BAP concentration was more suitable, resulting in a higher number of leaves. When the BAP concentration was 2 mg L-1, the explants exhibited greater length. Explants started to grow on nutrient medium containing 1.0 and 2.0 mg L-1 BAP by the fifth day and 73% of the explants reached a size suitable for subculturing by the end of the third week. The study found that varying the concentrations of IBA did not affect rooting rates. In the rooting medium containing 0.1 mg L-1 NAA, rooting success averaged 23.07% with 2.6 roots per plant for 'Ferragnes' and 62.50% rooting success with an average of 2 roots per plant for 'Ferraduel’.

Özet

Bu çalışmada, badem bahçe tesisinde kullanılan 'Ferragnes' ve 'Ferraduel' badem çeşitleri için uygun bir in vitro çoğaltım tekniği belirlenmiştir. Kültür ortamında sürgün gelişimini ve kardeşlenmeyi kolaylaştırmak için MS ortamına 1 mg L-1 ve 2 mg L-1 konsantrasyonlarında BAP ilave edilmiştir. 'Ferragnes' çeşidi için 1.0 mg L-1 BAP konsantrasyonunun daha uygun olduğu ve daha yüksek yaprak sayısına ulaşıldığı görülmüştür. BAP konsantrasyonu 2 mg L-1 olduğunda ise eksplantların uzunluğu artmıştır. 'Ferraduel' çeşidi için, 2.0 mg L-1 BAP kullanımının sürgün uzunluğu, genişliği, yaprak sayısı ve kardeşlenme oranı için uygun olduğu belirlenmiştir. Eksplantlar, beşinci gün itibariyle 1.0 ve 2.0 mg L-1 BAP içeren besin ortamında sürmeye başlamış ve eksplantların %73'ü üçüncü haftanın sonunda alt kültüre alınmaya uygun büyüklüğe ulaşmıştır. Çalışma, farklı IBA konsantrasyonlarının değişmesinin köklenme oranlarını etkilemediğini ortaya koymuştur. 'Ferragnes' için 0.1 mg L-1 NAA içeren köklendirme ortamında bitki başına 2.6 kök ile ortalama %23.07 köklenme başarısı ve 'Ferraduel' için bitki başına ortalama 2 kök ile % 62.50 köklenme başarısı elde edilmiştir.

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Cite This Article

Hammud, F., Ak, B. E., Hatipoglu, I. H., Ekinci, H., Dikmetaş, B. (2024). In vitro Propagation Techniques for 'Ferragnes' and 'Ferraduel' Almond Cultivars: Concentration Optimization and Rooting Success. Meyve Bilimi, Cilt: 11 Sayı: 2, 79–85. https://doi.org/10.51532/meyve.1564633

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