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Journals / Black Sea Journal of Agriculture / 2026 / Cilt: 9 Sayı: 3

Optimization of Culture Media for Shoot Regeneration and Rooting of Rosa chinensis var. viridiflora

Heydem Ekinci(Harran Üniversitesi)0000-0002-1828-7367
Cemre Kılınç0009-0005-9928-9717
Meral Doğan(HARRAN ÜNİVERSİTESİ)0000-0001-6227-2579
Necla Şaşkın(DİCLE ÜNİVERSİTESİ)0000-0003-3828-0522
Bekir Erol Ak(HARRAN ÜNİVERSİTESİ)0000-0001-6938-942X
Pages
360–368
Publication Date
2026-05-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings contribute to the development of in vitro propagation protocols for green roses.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Türkiye is an important genetic center for rose species, with approximately 45 different rose varieties found throughout the country.
📊
Key Findings & Data:In this study, different rooting media (R1: 0.5 mg L⁻¹ IBA + 25 µM SNP, R2: 0.5 mg L⁻¹ NAA + 25 µM SNP, and R3: 0.5 mg L⁻¹ IBA + 0.5 mg L⁻¹ NAA + 25 µM SNP) were used to determine the effects of different culture media (T1: Murashige and Skoog (MS), T2: Driver and Kuniyuki Walnut Medium (DKW), T3: Woody Plant Medium (WPM), T4: Nitsch and Nitsch (NN) on shoot development and rooting success of green rose explants.
💡
Conclusion & Impact:These findings contribute to the development of in vitro propagation protocols for green roses.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Medium optimization · Regeneration · Rooting

Abstract

Türkiye is an important genetic center for rose species, with approximately 45 different rose varieties found throughout the country. The green rose, native to China and scientifically known as Rosa chinensis var. viridiflora, grows in the Halfeti district of Şanlıurfa and is known as a rare ornamental plant synonymous with the region. Due to its limited distribution, the conservation and sustainable propagation of this variety are of great importance. Optimization of culture media is critical in the successful in vitro propagation of ornamental plants. In this study, different rooting media (R1: 0.5 mg L⁻¹ IBA + 25 µM SNP, R2: 0.5 mg L⁻¹ NAA + 25 µM SNP, and R3: 0.5 mg L⁻¹ IBA + 0.5 mg L⁻¹ NAA + 25 µM SNP) were used to determine the effects of different culture media (T1: Murashige and Skoog (MS), T2: Driver and Kuniyuki Walnut Medium (DKW), T3: Woody Plant Medium (WPM), T4: Nitsch and Nitsch (NN) on shoot development and rooting success of green rose explants. The study evaluated parameters such as the percentage of shoot-induction rate, shoot length, number of leaves, and number of nodes, as well as root diameter, root length, number of roots, and rooting rate. T4 medium showed the highest shoot-induction rate (22%), while T1 medium was superior in terms of shoot length (1.67 cm), number of leaves (11.27 per plantlet), and number of nodes (4.84 per plantlet). On the other hand, R1 medium provided the highest values in terms of root length (4.75 cm) and number of roots (2.75 per plantlet), while R3 medium increased root diameter (0.79 mm). Circular heatmap based on hierarchical clustering analysis comprehensively revealed the relative effects of the treatments. These findings contribute to the development of in vitro propagation protocols for green roses.

Özet

Türkiye is an important genetic center for rose species, with approximately 45 different rose varieties found throughout the country. The green rose, native to China and scientifically known as Rosa chinensis var. viridiflora, grows in the Halfeti district of Şanlıurfa and is known as a rare ornamental plant synonymous with the region. Due to its limited distribution, the conservation and sustainable propagation of this variety are of great importance. Optimization of culture media is critical in the successful in vitro propagation of ornamental plants. In this study, different rooting media (R1: 0.5 mg L⁻¹ IBA + 25 µM SNP, R2: 0.5 mg L⁻¹ NAA + 25 µM SNP, and R3: 0.5 mg L⁻¹ IBA + 0.5 mg L⁻¹ NAA + 25 µM SNP) were used to determine the effects of different culture media (T1: Murashige and Skoog (MS), T2: Driver and Kuniyuki Walnut Medium (DKW), T3: Woody Plant Medium (WPM), T4: Nitsch and Nitsch (NN) on shoot development and rooting success of green rose explants. The study evaluated parameters such as the percentage of shoot-induction rate, shoot length, number of leaves, and number of nodes, as well as root diameter, root length, number of roots, and rooting rate. T4 medium showed the highest shoot-induction rate (22%), while T1 medium was superior in terms of shoot length (1.67 cm), number of leaves (11.27 per plantlet), and number of nodes (4.84 per plantlet). On the other hand, R1 medium provided the highest values in terms of root length (4.75 cm) and number of roots (2.75 per plantlet), while R3 medium increased root diameter (0.79 mm). Circular heatmap based on hierarchical clustering analysis comprehensively revealed the relative effects of the treatments. These findings contribute to the development of in vitro propagation protocols for green roses.

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Cite This Article

Ekinci, H., Kılınç, C., Doğan, M., Şaşkın, N., Ak, B. E. (2026). Optimization of Culture Media for Shoot Regeneration and Rooting of Rosa chinensis var. viridiflora. Black Sea Journal of Agriculture, Cilt: 9 Sayı: 3, 360–368. https://doi.org/10.47115/bsagriculture.1899996

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