KaydedilenlerDerginizi ekleyin

Dergiler / International Journal of Chemistry and Technology / 2018 / Cilt: 2 - Sayı: 1

Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Yakup AKKOÇ
S. Seyhan TÜKEL
Sayfa
63–67
Yayın Tarihi
2018-06-27
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25°C, respectively.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:In this study, in order to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and glucose isomerase (GI) were immobilized together with cross-linked enzyme aggregates (CLEAs), and named as 'combi-CLEAs'.
📊
Temel Bulgular & Veri:The values of Vmaxand Kmfrom the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g-1 and 0.1157 mg ml-1 in optimum conditions, respectively.
💡
Sonuç & Katkı:At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25°C, respectively.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Combi-CLEA · HFS · Enzyme immobilization

Abstract

In this study, in order to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and glucose isomerase (GI) were immobilized together with cross-linked enzyme aggregates (CLEAs), and named as 'combi-CLEAs'. The values of Vmaxand Kmfrom the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g-1 and 0.1157 mg ml-1 in optimum conditions, respectively. After 50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL + GI] was preserved at 46%. The thermal stability of combi-CLEAs [GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C after 24 hours. At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25°C, respectively.

🔖

Bu Makaleye Atıf Yap

AKKOÇ, Y., TÜKEL, S. S. (2018). Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. International Journal of Chemistry and Technology, Cilt: 2 - Sayı: 1, 63–67. https://ajindex.com/articles/765721

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (14)

  1. Schindler, I.; Renz, A.; Schmid, F.X.;Beck, E. Biochım. Biophys. Acta 2001,1548, 175-186
  2. Cha, X.; Zhou, H.; Li, W.; Shen, J. J. Chem. Technol. Biot. 1990, 47, 161-169
  3. Schafhauser, D.Y.; Storey, K.B. Appl. Biochem. Biotechnol. 1992, 36, 63-74
  4. Yubin,Ge.; Yingming, W.; Hui, Zhou, S. W.; Yi, T.; Wei, L. J. Biotechnol. 1999, 67, 33-34
  5. Sohel, D.; Manali, K.; Munishwar, N. G. J. Mol. Catal. B-Enzym. 2007, 44, 128-132
  6. Sachin, T.; Amol, P.; Mayur, L.; Shamraja, N.; Mosin, M.; Kshitija, J.; Kishori,P.; Devika, A. Bioresour. Technol. 2013, 147, 269-275
  7. Jolanta, B. Biochem. Eng. J. 2003,16, 347-355
  8. Qiaolin, L.; Long, Y.; Jianguo, S.; Liang, L.; Lin, X.; Aihua, Li. Biosens. Bioelectron.2014, 51, 158-163
  9. Gupta, M.N.; Dalal, S.; Kapoor, M. J. Mol. Catal. Enzyme B. 2017, 44, 128-132
  10. Tolsma, A. D. The effects of fire and grazing on the energy reserves of resprouting plants in Victoria's alpine grasslands. PhD Thesis, School of Resource Management, Forestry and Amenity Horticulture, The University of Melbourne, 2002
  11. Roushdi, M.; Ghali, Y.; Attia, R. M.; Alaa El-din, M. Starch/Stärke. 1979, 31 (12), 414-417
  12. Katwa, L.C.: Raghavendra, M.R. Technol. Lett. 1983, 5, 191-196
  13. Mıshra, A.; Debnath, M. App. Biochem. Biotechnol. 2002, 102-103
  14. Michaelis, L.; Menten, M. Biochem. Zeitung, 1913, 49, 333-369.