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Dergiler / International Journal of Chemistry and Technology / 2026 / Volume: 2 Issue: 1

Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Yakup Akkoç(ARDAHAN ÜNİVERSİTESİ)0000-0002-2622-1341
S. Seyhan Tükel(ÇUKUROVA ÜNİVERSİTESİ)0000-0003-2503-2781
Sayfa
—
Yayın Tarihi
2018-06-27
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At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25 ° C, respectively.

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Amaç & Hipotez:In this study, in order to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and glucose isomerase (GI) were immobilized together with cross-linked enzyme aggregates (CLEAs), and named as 'combi-CLEAs'.
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Temel Bulgular & Veri:The values of V max and K m from the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g -1 and 0.1157 mg ml -1 in optimum conditions, respectively.
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Sonuç & Katkı:At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25 ° C, respectively.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Combi-CLEAs · fructose syrup · enzyme immobilization

Abstract

In this study, in order to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and glucose isomerase (GI) were immobilized together with cross-linked enzyme aggregates (CLEAs), and named as 'combi-CLEAs'. The values of V max and K m from the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g -1 and 0.1157 mg ml -1 in optimum conditions, respectively. After 50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL + GI] was preserved at 46%. The thermal stability of combi-CLEAs [GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C after 24 hours. At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25 ° C, respectively.

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Bu Makaleye Atıf Yap

Akkoç, Y., Tükel, S. S. (2018). Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. International Journal of Chemistry and Technology, Volume: 2 Issue: 1. https://doi.org/10.32571/ijct.420772

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