Effects Of Pınealectomy On Morphologıcal Features Of Blood Vessel In Chıcken: An Electron Mıcroscopıc And Stereologıcal Study

In the light of current study, the structural changes in the vessel demonstrated by quantitative morphometric methods have been interpreted as a reflection of the role of melatonin on vessel wall and vascular reactivity, but this suggestion need to be validated in the human setting.

📌 3 Maddede Bu Makale

🎯

Amaç & Hipotez:Although the effects of melatonin on vascular smooth muscle and vascular reactivity are described in last decade, the effects of melatonin on vascular tissues are still vague.

📊

Temel Bulgular & Veri:Fifteen chicks were divided into three groups, namely unoperated control, sham-operated and pinealectomized chicks.

💡

Sonuç & Katkı:In the light of current study, the structural changes in the vessel demonstrated by quantitative morphometric methods have been interpreted as a reflection of the role of melatonin on vessel wall and vascular reactivity, but this suggestion need to be validated in the human setting.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Artery · Chicken · Electron microscopy

Abstract

Although the effects of melatonin on vascular smooth muscle and vascular reactivity are described in last decade, the effects of melatonin on vascular tissues are still vague. The current study investigated the effects of pinealectomy on the blood vessel wall in the chicken. Fifteen chicks were divided into three groups, namely unoperated control, sham-operated and pinealectomized chicks. At the end of the experiment, a quantitative structural analysis of 15 sciatic artery samples from these groups was investigated. Effects of pinealectomy were quantified using volume fraction approach of stereological method. In this study, total cross-sectional area of the artery in pinealectomy group was significantly higher than those in unoperated control and sham-operated animals (p < 0.05). However, surgical pinealectomy procedure resulted in a significantly decreased thickness of the tunica adventitia of the vessel wall, in contrast to those of tunica media (p < 0.01). The present results indicate that pinealectomy had a vasodilator effect on the sciatic artery. In the light of current study, the structural changes in the vessel demonstrated by quantitative morphometric methods have been interpreted as a reflection of the role of melatonin on vessel wall and vascular reactivity, but this suggestion need to be validated in the human setting.

Anahtar kelimeler

Artery Chicken Electron microscopy Stereology Pinealectomy Melatonin

🔖

Bu Makaleye Atıf Yap

TURGUT, M., KAPLAN, S., ÜNAL, Z., ALTUNKAYNAK, Z., ÜNAL, D., ŞAHİN, B., BOZKURT, M., YURTSEVEN, M. (2010). Effects Of Pınealectomy On Morphologıcal Features Of Blood Vessel In Chıcken: An Electron Mıcroscopıc And Stereologıcal Study. Journal of Experimental and Clinical Medicine, Cilt: 26 - Sayı: 3, 112–118. https://ajindex.com/articles/679130

📄 Tam Metin PDF Okuyucu

Yeni Sekmede Aç ↗

↗Yeni Sekme

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (13)

Anwar, M.M., Meki, A.R., Rahma, H.H. 2001. Inhibitory effects of melatonin on vascular reactivity: possible role of vasoactive mediators. Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 130, 357-367.
Chucharoen, P., Chetsawang, B., Srikiatkhachorn, A., Govitrapong, P. 2003. Melatonin receptor expression in rat cerebral artery. Neurosci. Lett. 341, 259-261.
Cuzzocrea, S., Costantino, G., Mazzon, E., Micali, A., De Sarro, A., Caputi, A.P. 2000. Beneficial effects of melatonin in a rat model of splanchnic artery occlusion and reperfusion. J. Pineal Res. 28, 52-63.
Doolen, S., Krause, D.N., Dubocovich, M.L., Duckles, S.P. 1998. Melatonin mediates two distinct responses in vascular smooth muscle. Eur. J. Pharmacol. 345, 67-69.
Ekmekcioglu, C., Haslmayer, P., Phillips, C., Mehrabi, M.R., Glogar, H.D., Grimm, M., Thalhammer, T., Marktl, W. 2001. 24h variation in the expression of the mt1 melatonin receptor subtype in coronary arteries derived from patients with coronary heart disease. Chronobiol. Int. 18, 973-985.
Geary, G.G., Duckles, S.P., Krause, D.N. 1998. Effect of melatonin in the rat tail artery: role of K+ channels and endothelial factors. Br. J. Pharmacol. 123, 1533-1540.
Gundersen, H.J.G. 1986. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J. Microsc. 143, 3-45.
Hadju, M.A., Heistad, D.D., Siems, J.E., Baumbach, G.L. 1990. Effects of aging on mechanics and composition of cerebral arterioles in rats. Circ. Res. 66, 1747-1754.
Joo, J.Y., Uz, T., Manev, H. 1998. Opposite effects of pinealectomy and melatonin administration on brain damage following cerebral focal ischemia in rat. Restor Neurol Neurosci. 13, 185-191.
Kaplan, S., Canan, S., Aslan, H., Ünal, B., Şahin, B. 2001. A simple technique to measure the movements of the microscope stage along the x and y axes for stereological methods. J. Microsc. 203, 321-325.
Kaplan, S., Gokyar, A., Unal, B., Tunç, A.T., Bahadır, A., Aslan, H. 2005. A simple technique for localizing consecutive fields for disector pairs in light microscopy: Application to neuron counting in rabbit spinal cord following spinal cord injury. J. Neurosci. Methods. 145, 277-284.
Laitinen, J.T, Viswanathan, M., Vakkuri, O., Saavedra, J.M. 1992. Differential regulation of the rat melatonin receptors: selective age-associated decline and lack of melatonin-induced changes. Endocrinology. 130, 2139-2144.
Mahle, C.D., Goggins, G.D., Aga