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Dergiler / Journal of Experimental and Clinical Medicine / 2021 / Cilt: 38 - Sayı: 3

Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

Ayşe ALTUN
Yasemin ERSOY CANILLIOĞLU
Evrim UNSAL
Seda KARABULUT
Canan HURDAG
Sayfa
272–276
Yayın Tarihi
2021-05-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Spermatogenesis is a process that involves cellular and biochemical processes.
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Temel Bulgular & Veri:The purpose of this study is to understand the role of tight junctions in spermatogenesis by evaluating Zonula occludens-1 (ZO-1) protein levels of cells from the testicular sperm extraction (TESE) biopsies in patients with azoospermia The study included 40 infertile men with azoospermia.
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Sonuç & Katkı:Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:TESE · Azoospermia · Tight juntion proteins ZO-1

Abstract

Spermatogenesis is a process that involves cellular and biochemical processes. The purpose of this study is to understand the role of tight junctions in spermatogenesis by evaluating Zonula occludens-1 (ZO-1) protein levels of cells from the testicular sperm extraction (TESE) biopsies in patients with azoospermia The study included 40 infertile men with azoospermia. They were divided into two groups, as spermatozoa positive (n=20) and spermatozoa negative (n=20). Testis tissues were examined morphologically, immunohistochemically and ultrastructurally by using transmission electron microscopy (TEM). The structure of seminiferous tubules were deteriorated, degenerated and hyalinized in spermatozoa negative group. ZO-1 expression level was normal in blood-testis barrier of spermatozoa positive group in comparing to lateral surfaces of sertoli cells spermatozoa negative group. The ultrastructural results were also supported with light microscopy findings. These findings suggest that decrease of the ZO-1 protein expression level in Sertoli cells might be one of the factors involved in the impairment of spermatogenesis in spermatozoa negative group. Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.

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Bu Makaleye Atıf Yap

ALTUN, A., CANILLIOĞLU, Y. E., UNSAL, E., KARABULUT, S., HURDAG, C. (2021). Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. Journal of Experimental and Clinical Medicine, Cilt: 38 - Sayı: 3, 272–276. https://ajindex.com/articles/677979

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Kaynakça (11)

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