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Dergiler / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 7 Sayı: 3

Topraktan İzole Edilen Streptomyces sp.’den Ksilanaz Üretimi ve Karakterizasyonu

Production and Characterization of Xylanase from Streptomyces sp. which is Isolated from Soil

Barış Enez
Sema Agüloğlu Fincan
Sayfa
115–123
Yayın Tarihi
2017-09-30
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Streptomyces sp.’nin optimum şartları belirlenerek optimum 35 o C, pH 7.0 ve 52. saat olarak maksimum üreme gerçekleştirdiği tespit edilmiştir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Bu çalışmada topraktan izole edilen Streptomyces sp.’nin izolasyonu, tanımlanması ve optimizasyonu gerçekleştirilmiştir.
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Temel Bulgular & Veri:Streptomyces sp.’nin optimum şartları belirlenerek optimum 35 o C, pH 7.0 ve 52. saat olarak maksimum üreme gerçekleştirdiği tespit edilmiştir.
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Sonuç & Katkı:Kullanılan deterjanlardan Triton X-100 aktiviteyi arttırırken SDS ise güçlü şekilde inhibe ettiği tespit edilmiş ve enzim aktivitesinin; 35 o C-45 o C aralıklarında da 1 saat boyunca stabil kaldığı, pH 6.0, ve 7.0 de 1 saat sonunda %100 korunduğu görülmüştür
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Streptomyces sp. · ksilanaz · izolasyon

Özet

Bu çalışmada topraktan izole edilen Streptomyces sp.’nin izolasyonu, tanımlanması ve optimizasyonu gerçekleştirilmiştir. Streptomyces sp.’nin optimum şartları belirlenerek optimum 35 o C, pH 7.0 ve 52. saat olarak maksimum üreme gerçekleştirdiği tespit edilmiştir. Ksilanaz üretiminin en iyi olduğu koşullar ise 35 o C, pH 7.0 ve 72. saat olarak belirlenmiş ve en iyi enzim aktivitesine 40 o C ve pH 7.0’de ulaşılmıştır. Enzim aktivitesi üzerine metallerin etkisi araştırıldığında Zn 2+ , Hg 2+ ve Fe 2+ elementlerinin güçlü bir şekilde enzim aktivitesini inhibe ettiği gözlemlenmiştir. Kullanılan deterjanlardan Triton X-100 aktiviteyi arttırırken SDS ise güçlü şekilde inhibe ettiği tespit edilmiş ve enzim aktivitesinin; 35 o C-45 o C aralıklarında da 1 saat boyunca stabil kaldığı, pH 6.0, ve 7.0 de 1 saat sonunda %100 korunduğu görülmüştür

Abstract

In this work, Streptomyces sp., was isolated from soil, identifed and optimized. Optimum conditions of Streptomyces sp. were determined and it is observed that maximum reproduction is achieved at 35 o C, pH 7.0 and 52 hours. It was also revealed that the best conditions for producing xylanase was 35 o C, pH 7.0 and 72 hours and the best enzyme activity was achieved at 40 °C and pH 7.0. The effect of metals on enzyme activity was investigated, and it was observed that enzyme activity was strongly inhibited by Zn 2+ , Hg 2+ and Fe 2+ . It was found that Triton X-100, one of the detergents was used, boosts activity, while other one, SDS, inhibits strongly and it is revealed that, enzyme activity remains stable for 1 hour at 35 o C, 40 o C and 45 o C and also it is preserved 100% after 1 hour at pH 6.0 and pH 7.0 .

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Bu Makaleye Atıf Yap

Enez, B., Fincan, S. A. (2017). Topraktan İzole Edilen Streptomyces sp.’den Ksilanaz Üretimi ve Karakterizasyonu. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 7 Sayı: 3, 115–123. https://ajindex.com/articles/1272997

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