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Dergiler / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 7 Sayı: 3

Salda Gölü Prokaryotik Çeşitliliğinin Yeni Nesil Dizileme Yöntemiyle Belirlenmesi

Determination Of Prokaryotic Diversity Of Salda Lake By Next Generation Sequencing Method

Nilgün Poyraz
Mehmet Burçin Mutlu
Sayfa
99–106
Yayın Tarihi
2017-09-30
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Halobacteria sınıfının Halobacterialaes ordosu Halobacteriaceae familyasının Haloarcula , Halobacterium , Halonotius , Halorhabdus , Halorubrum ve Haloplanus cinsi üyeleri de baskın olarak bulunmaktadır

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Çalışmada Salda Gölü’nden örnekleme yapılmış ve örnek için pH ve tuzluluk değerleri belirlenmiştir.
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Temel Bulgular & Veri:Analiz sonrası elde edilen veriler değerlendirildiğinde en baskın bulunan Bacteria flumlarının Proteobacteria ve Actinobacteria olduğu belirlenmiştir.
💡
Sonuç & Katkı:Halobacteria sınıfının Halobacterialaes ordosu Halobacteriaceae familyasının Haloarcula , Halobacterium , Halonotius , Halorhabdus , Halorubrum ve Haloplanus cinsi üyeleri de baskın olarak bulunmaktadır
🔬Tür:Uygulamalı Anket & Alan Araştırması
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alkaliflik · prokaryotik çeşitlilik · Salda Gölü

Özet

Çalışmada Salda Gölü’nden örnekleme yapılmış ve örnek için pH ve tuzluluk değerleri belirlenmiştir. Sonrasında örnekler fltreden geçirilerek, bu fltrelerden DNA ekstraksiyonu işlemi yapılmıştır. Elde edilen DNA Illumina MiSeq dizileme sistemiyle dizilenmiş ve veriler QIIME Programı ile analiz edilmiştir. Analiz sonrası elde edilen veriler değerlendirildiğinde en baskın bulunan Bacteria flumlarının Proteobacteria ve Actinobacteria olduğu belirlenmiştir. Sınıf düzeyinde ise Gammaproteobacteria, Acidimicrobia ve Actinobacteria baskındır. Ordo düzeyinde ise Enterobacteriales, Acidimicrobiales, Actinobacteriales baskındır. Archaea domaini değerlendirildiğinde ise Euryarchaeota flumunun baskın olduğu belirlenmiştir. Sınıf düzeyinde Halobacteria üyeleri baskındır. Halobacteria sınıfının Halobacterialaes ordosu Halobacteriaceae familyasının Haloarcula , Halobacterium , Halonotius , Halorhabdus , Halorubrum ve Haloplanus cinsi üyeleri de baskın olarak bulunmaktadır

Abstract

In our study, water sample was taken from Salda Lake and the pH and salinity data for the sample were determined. After that, samples were fltered and DNA extraction was performed. The obtained DNA was sequenced by the Illumina MiSeq sequencing system and data was analyzed by the QIIME program. When the data obtained after the analysis were evaluated, it was determined that the most dominant bacterial phyla are Proteobacteria and Actinobacteria. At class level, Gammaproteobacteria, Acidimicrobia and Actinobacteria were dominant. In order level Enterobacteriales, Acidimicrobiales, Actinobacteriales were abundant. When the Archaea domain was evaluated it was determined that the Euryarchaeota phylum was dominant. Halobacteria members were abundant at the class level. In addition Halobacteria class, Halobacteriales order, Halobacteriaceae family includes Haloarcula , Halobacterium , Halonotius , Halorhabdus , Halorubrum and Haloplanus genus members

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Bu Makaleye Atıf Yap

Poyraz, N., Mutlu, M. B. (2017). Salda Gölü Prokaryotik Çeşitliliğinin Yeni Nesil Dizileme Yöntemiyle Belirlenmesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 7 Sayı: 3, 99–106. https://ajindex.com/articles/1272995

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