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Dergiler / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 8 Sayı: 1

Yenilebilir Dört Bitki Türünün Fitokimyasal İçeriğinin Miktarsal Tayini için LC-MS/MS Metot Validasyonu

An LC-MS/MS Method Validation for the Phytochemical Quantification of Four Edible Plants

Mustafa Abdullah Yılmaz0000-0002-4090-7227
Oguz Çakır0000-0002-8006-2054
Enes Arıca0000-0002-8663-4826
İsmail Yener0000-0002-0988-9462
Birol Otludil0000-0002-3809-5987
Mehmet Hakkı Alma0000-0001-7011-3965
Abdulselam Ertaş0000-0002-2193-8386
Sayfa
179–187
Yayın Tarihi
2018-03-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bitkilerde 8 fitokimyasal bileşiğin (sinarin, kafeik asit, sirinjik asit, p-kumarik asit, o-kumarik asit, vanilik

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Bitkilerde 8 fitokimyasal bileşiğin (sinarin, kafeik asit, sirinjik asit, p-kumarik asit, o-kumarik asit, vanilik
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Temel Bulgular & Veri:Bitkilerde 8 fitokimyasal bileşiğin (sinarin, kafeik asit, sirinjik asit, p-kumarik asit, o-kumarik asit, vanilik
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Sonuç & Katkı:Bitkilerde 8 fitokimyasal bileşiğin (sinarin, kafeik asit, sirinjik asit, p-kumarik asit, o-kumarik asit, vanilik
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Fitokimyasal · LC-MS/MS · metot validasyonu

Özet

Bitkilerde 8 fitokimyasal bileşiğin (sinarin, kafeik asit, sirinjik asit, p-kumarik asit, o-kumarik asit, vanilik

Abstract

A comprehensive LC-MS/MS method was developed and validated for the quantification of eight plant phytochemicals (cynarin, caffeic acid, syringic acid, p-coumaric acid, o-coumaric acid, vanillic acid, ferulic acid, chlorogenic acid) in plants. The developed analytical method was fully validated in terms of linearity, accuracy (recovery), inter and intra-day precision (repeatability), limits of detection and quantification (LOD/LOQ) and relative standard uncertainty (U% at 95% confidence level (k=2)). Chromatographic separation was performed on a reverse phased UHPLC. MS detection was performed using a triple quadrupole mass spectrometer and negative or positive ionization modes were optimized for each analyte. Multiple reaction monitoring (MRM) was used to quantify the analytes, related molecular ions and transition ions were optimized. After method validation, the phytochemical composition of methanolic extracts of some edible plants including artichoke (Cynara scolymus L.), broccoli (Brassica Oleracea var. Italica), cauliflower (Brassica Oleracea var. Botrytis) and tumble thistle (Gundelia Tournefortii) were investigated by the developed and validated LC-MS/MS method. Among the analysed plants, artichoke was by far the richest one in terms of phenolics. Additionally, chlorogenic acid was the most abundant phenolic compound in all plants. Although the studied edible plants were chosen as real samples, the developed LC-MS/MS method is applicable to a wide range of species in plant kingdom.

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Bu Makaleye Atıf Yap

Yılmaz, M. A., Çakır, O., Arıca, E., Yener, İ., Otludil, B., Alma, M. H., Ertaş, A. (2018). Yenilebilir Dört Bitki Türünün Fitokimyasal İçeriğinin Miktarsal Tayini için LC-MS/MS Metot Validasyonu. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 1, 179–187. https://doi.org/10.21597/jist.407870

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