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Dergiler / Kommagene Biyoloji Dergisi / 2024 / Cilt: 8 Sayı: 2

Micromeria myrtifolia Boiss. & Hohen. Bitkisinin Farmakolojik Potansiyelinin Araştırılması: Fenolik İçeriğinin ve Biyolojik Aktivitelerinin Belirlenmesi

Investigating the Pharmacological Potential of Micromeria myrtifolia Boiss. & Hohen.: Phenolic Profiling and Biological Activity Assessments

Ezgi Ersoy(BIRUNI UNIVERSITY, FACULTY OF PHARMACY)0000-0002-6913-1952
Rümeysa Batal Yıldızhan(DICLE UNIVERSITY, FACULTY OF PHARMACY)0009-0006-8211-9073
Mustafa Abdullah Yılmaz(DICLE UNIVERSITY, FACULTY OF PHARMACY)0000-0002-4090-7227
Yeter Yeşil(ISTANBUL UNIVERSITY, FACULTY OF PHARMACY, PHARMACY PR.)0000-0002-4458-7881
Hasan Şahin(DICLE UNIVERSITY, FACULTY OF PHARMACY)0000-0002-8325-8116
Esra Eroğlu Özkan(ISTANBUL UNIVERSITY, FACULTY OF PHARMACY, PHARMACY PR.)0000-0002-1569-2535
Mehmet Boğa(DICLE UNIVERSITY, FACULTY OF PHARMACY)0000-0003-4163-9962
Sayfa
103–114
Yayın Tarihi
2024-12-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Her iki ekstrede de ana bileşen olarak nikotiflorin öne çıkmış ve topraküstü kısımlarından hazırlanan ekstrede 12878.31±355.44 μg/g, kök ekstresinde ise 47512.4±1311.34 μg/g nikotiflorin tespit edilmiştir.

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Amaç & Hipotez:Micromeria myrtifolia Boiss. & Hohen., Türkiye'nin önemli tıbbi bitkilerindendir ve ülke genelinde farklı tıbbi amaçlarla oldukça yaygın olarak kullanılmaktadır.
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Temel Bulgular & Veri:Her iki ekstrede de ana bileşen olarak nikotiflorin öne çıkmış ve topraküstü kısımlarından hazırlanan ekstrede 12878.31±355.44 μg/g, kök ekstresinde ise 47512.4±1311.34 μg/g nikotiflorin tespit edilmiştir.
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Sonuç & Katkı:Bu çalışma, M. myrtifolia bitkisinin kimyasal içeriği ve biyolojik aktiviteleri konusunda mevcut literatüre önemli katkılarda bulunmakla birlikte, bitkinin geleneksel ve modern tıp uygulamalarındaki yeni ve potansiyel kullanım alanlarını keşfetmeye yönelik gelecek araştırmalara da zemin hazırlamaktadır.
🔬Tür:Literatür & Derleme Çalışması
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Micromeria Benth. · LC-MS/MS · Antioksidan

Özet

Micromeria myrtifolia Boiss. & Hohen., Türkiye'nin önemli tıbbi bitkilerindendir ve ülke genelinde farklı tıbbi amaçlarla oldukça yaygın olarak kullanılmaktadır. Bu çalışmada, M. myrtifolia örnekleri Muğla, Türkiye'den toplanmış hem topraküstü kısımlarından hem de köklerden ayrı ayrı etanol ekstreleri hazırlanmıştır. Ekstrelerin fitokimyasal içeriklerini belirlemek için detaylı bir LC-MS/MS analizi yapılmıştır. Analiz sonuçlarına göre her iki ekstrenin de fenolik bileşikler açısından oldukça zengin olduğu belirlenmiştir. Her iki ekstrede de ana bileşen olarak nikotiflorin öne çıkmış ve topraküstü kısımlarından hazırlanan ekstrede 12878.31±355.44 μg/g, kök ekstresinde ise 47512.4±1311.34 μg/g nikotiflorin tespit edilmiştir. Ayrıca ekstrelerde rozmarinik asit ve kafeik asit gibi önemli fenolik asitler ile hesperidin gibi flavonoitlerin varlığı da belirlenmiştir. Ekstrelerin antioksidan aktiviteleri DPPH serbest radikal giderici, ABTS katyon radikali giderici ve CUPRAC aktivitesi metotları ile değerlendirilmiş, buna göre her iki ekstre de sentetik antioksidan bileşiklere kıyasla anlamlı antioksidan aktiviteler sergilemiştir. Bunun yanı sıra ekstreler enzim inhibisyon aktiviteleri açısından değerlendirildiğinde, ekstrelerin 2 mg/mL konsantrasyonda %73.03±0.16 (topraküstü) ve %47.06±0.41 (kök) oranında anti-glukozidaz aktivite gösterdiği tespit edilmiştir. Kök ekstresi ayrıca orta düzeyde butirilkolinesteraz inhibe edici aktivite de göstermiştir. Bu çalışma, M. myrtifolia bitkisinin kimyasal içeriği ve biyolojik aktiviteleri konusunda mevcut literatüre önemli katkılarda bulunmakla birlikte, bitkinin geleneksel ve modern tıp uygulamalarındaki yeni ve potansiyel kullanım alanlarını keşfetmeye yönelik gelecek araştırmalara da zemin hazırlamaktadır.

Abstract

Micromeria myrtifolia Boiss. & Hohen. is a valuable medicinal plant in Türkiye, recognized for its extensive applications across the country. In this study, plant samples were collected from Muğla, Türkiye and extracts from both the aerial parts and roots were prepared using ethanol. To elucidate their phytochemical composition, a comprehensive LC-MS/MS analysis was conducted. The results revealed that both extracts were quite rich in phenolic compounds. Notably, nicotiflorin was the major constituent in both extracts (12878.31355.44 μg/g extract in the aerial parts and 47512.41311.34 μg/g extract in the roots) along with significant phenolic acids such as rosmarinic acid and caffeic acid as well as flavonoids like hesperidin. Moreover, both extracts demonstrated substantial antioxidant activities compared to the synthetic antioxidant compounds as evaluated by DPPH free radical scavenging, ABTS cation radical scavenging, and CUPRAC activity assays. Both aerial parts and root extracts also exhibited meaningful anti-glucosidase activity with 73.030.16% and 47.060.41%, respectively, at 2 mg/mL concentration. The root extract also showed moderate butyrylcholinesterase inhibitory activity. This study contributes to the existing body of knowledge with valuable insights regarding the phytochemical profile and biological activities of M. myrtifolia, paving the way for future research aimed at exploring its medicinal properties and potential uses in traditional and modern medicine.

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Bu Makaleye Atıf Yap

Ersoy, E., Yıldızhan, R. B., Yılmaz, M. A., Yeşil, Y., Şahin, H., Özkan, E. E., Boğa, M. (2024). Micromeria myrtifolia Boiss. & Hohen. Bitkisinin Farmakolojik Potansiyelinin Araştırılması: Fenolik İçeriğinin ve Biyolojik Aktivitelerinin Belirlenmesi. Kommagene Biyoloji Dergisi, Cilt: 8 Sayı: 2, 103–114. https://doi.org/10.31594/commagene.1576852

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Kaynakça (66)

  1. Abu-Gharbieh, E., & Ahmed, N.G. (2016). Bioactive content, hepatoprotective, and antioxidant activities of whole plant extract of Micromeria fruticosa (L.) Druce ssp. Serpyllifolia F. (Lamiaceae) against carbon tetrachloride-induced hepatotoxicity in mice. Tropical Journal of Pharmaceutical Research, 15(10), 2099-2106. http://doi.org/10.4314/tjpr.v15i10.7
  2. Adjdir, S., Benariba, N., El Haci, I. A., Ouffai, K., Bekkara, F. A., & Djaziri, R. (2021). Antioxidant activity and phenolic compounds identification of Micromeria inodora (Desf.) Benth. from Western Algeria. Natural Product Research, 35(17), 2963-2966. https://doi.org/10.1080/14786419.2019.1678612
  3. Aleixandre, A., Gil, J.V., Sineiro, J., & Rosell, C.M. (2022). Understanding phenolic acids inhibition of α-amylase and α-glucosidase and influence of reaction conditions. Food Chemistry, 372, 131231. https://doi.org/10.1016/j.foodchem.2021.131231
  4. Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E. (2004). Novel total antioxidant capacity index for diet arypoly phenols and vitamin C and E, using their cupricion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981. https://doi.org/10.1021/jf048741x
  5. Baykan, S., Ozturk, B., Sahin, B., & Senol, S.G. (2023). Ethnobotanical study of medicinal plants in Nemrut Mountain, Adiyaman-Türkiye. Journal of Research in Pharmacy, 27(6). http://dx.doi.org/10.29228/jrp.514
  6. Bektaş, N.Y., Ersoy, E., Boğa, M., Boran, T., Çinar, E., Özhan, G.,. & Özkan, E.E. (2021). Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry, 45(5), 1621-1638. https://doi.org/10.3906/kim-2104-17
  7. Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  8. Boga, M., Ersoy, E., Ozkan, E.E., Cinar, E., Kara, E.M., Canturk, Y.Y., & Zengin, G. (2021). Volatile and phenolic profiling of a traditional medicinal plant, Hypericum empetrifolium with in vitro biological activities. Journal of Ethnopharmacology, 272, 113933. https://doi.org/10.1016/j.jep.2021.113933
  9. Brahmi, F., Guendouze, N., Hauchard, D., Okusa, P., Kamagaju, L., Madani, K., & Duez, P. (2017). Phenolic profile and biological activities of Micromeria graeca (L.) Benth. ex Rchb. International Journal of Food Properties.20(sup2), 2070-2083. https://doi.org/10.1080/10942912.2017.1362650
  10. Duman, H., & Dirmenci, T. (2017). A new species of Micromeria (Lamiaceae) from Köyceğiz (Muğla, southwest of Turkey). Turkish Journal of Botany, 41(4), 383-391. https://doi.org/10.3906/bot-1612-49
  11. El Kamari, F., El Omari, H., El-Mouhdi, K., Chlouchi, A., Harmouzi, A., Lhilali, I.,. & Ousaaid, D. (2024). Effects of Different Solvents on the Total Phenol Content, Total Flavonoid Content, Antioxidant, and Antifungal Activities of Micromeria graeca L. from Middle Atlas of Morocco. Biochemistry Research International, 2024(1), 9027997. https://doi.org/10.1155/2024/9027997
  12. Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 17, 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  13. Eroglu Ozkan, E., Ersoy, E., Yesil Canturk, Y., Mataraci Kara, E., Cinar, E., Sahin, H.,. & Boga, M. (2022). The therapeutic potential of Ethnomedicinally important Anatolian Thyme species: a Phytochemical and Biological Assessment. Frontiers in Pharmacology, 13, 923063. https://doi.org/10.3389/fphar.2022.923063
  14. Ersoy, E., Karahan, S., Boğa, M., Çınar, E., İzgi, S., Kara, E. M.,. & Özkan, E. E. (2022). Evaluation of the medicinal potential of a traditionally important plant from Turkey: Cerinthe minor L. Istanbul Journal of Pharmacy, 52(1), 80-89. https://doi.org/10.26650/IstanbulJPharm.2022.1001685
  15. Ersoy, E., Ozkan, E.E., Boga, M., & Mat, A. (2020). Evaluation of in vitro biological activities of three Hypericum species (H. calycinum, H. confertum, and H. perforatum) from Turkey. South African Journal of Botany, 130, 141-147. https://doi.org/10.1016/j.sajb.2019.12.017
  16. Ersoy, E., Ozkan, E.E., Karahan, S., Şahin, H., Cinar, E., Canturk, Y.Y.,. & Boga, M. (2023). Phytochemical analysis of essential oils and the extracts of an ethnomedicinal plant, Teucrium multicaule collected from two different locations with focus on their important biological activities. South African Journal of Botany, 153, 124-135. https://doi.org/10.1016/j.sajb.2022.12.020
  17. Formisano, C., Oliviero, F., Rigano, D., Saab, A.M., & Senatore, F. (2014). Chemical composition of essential oils and in vitro antioxidant properties of extracts and essential oils of Calamintha origanifolia and Micromeria myrtifolia, two Lamiaceae from the Lebanon flora. Industrial Crops and Products, 62, 405-411. https://doi.org/10.1016/j.indcrop.2014.08.043
  18. Güzel, Y., Güzelşemme, M., & Miski, M. (2015). Ethnobotany of medicinal plants used in Antakya: a multicultural district in Hatay Province of Turkey. Journal of Ethnopharmacology, 174, 118-152. https://doi.org/10.1016/j.jep.2015.07.042
  19. Hamwi, M., & El-Lakany, A. (2021). Genus Micromeria: A Review Article. BAU Journal of Health and Wellbeing, 3(2), 5. https://doi.org/10.54729/2789-8288.1130
  20. Kocabaş, Y.Z., & Gedik, O. (2016). An Ethnobotanical Study of wild plants sold in district bazaar in Kahramanmaras. Journal of the Institute of Science and Technology, 6(4), 41-50. https://doi.org/10.54729/2789-8288.1130
  21. Küpeli Akkol, E., Gürağaç Dereli, F.T., & Ilhan, M. (2019). Assessment of antidepressant effect of the aerial parts of Micromeria myrtifolia Boiss. & Hohen on mice. Molecules, 24(10), 1869. https://doi.org/10.3390/molecules24101869
  22. Lawag, I.L., Aguinaldo, A.M., Naheed, S., & Mosihuzzaman, M. (2012). α-Glucosidase inhibitory activity of selected Philippine plants. Journal of Ethnopharmacology, 144(1), 217-219. https://doi.org/10.1016/j.jep.2012.08.019
  23. Li, R., Guo, M., Zhang, G., Xu, X., & Li, Q. (2006). Nicotiflorin reduces cerebral ischemic damage and upregulates endothelial nitric oxide synthase in primarily cultured rat cerebral blood vessel endothelial cells. Journal of Ethnopharmacology, 107(1), 143-150. https://doi.org/10.1016/j.jep.2006.04.024
  24. Masamoto, Y., Kubo, M. (1980). Inhibitory effect of Chinese crude drugs on tyrosinase. Planta Medica, 40(12), 361-365. https://doi.org/10.1055/s-2008-1074986
  25. Mladenova, T., Stoyanov, P., Denev, P., Dimitrova, S., Katsarova, M., Teneva, D.,. & Bivolarska, A. (2021). Phytochemical Composition, Antioxidant and Antimicrobial Activity of the Balkan Endemic Micromeria frivaldszkyana (Degen) Velen. (Lamiaceae). Plants, 10(4), 710. https://doi.org/10.3390/plants10040710
  26. Mohammadhosseini, M., Venditti, A., Flamini, G., Sarker, S.D., & Kalaee, M. (2022). The genus Micromeria Benth.: An overview on ethnobotany, chemotaxonomy and phytochemistry. Trends in Phytochemical Research, 6(3), 155-186. https://doi.org/10.30495/tpr.2022.694114
  27. Moreno, M.I.N., Isla, M.I., Sampietro, A.R., Vattueno, M.A. (2000). Comparison of the free radicals cavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  28. Mykhailenko, O., Gudžinskas, Z., Kovalyov, V., Desenko, V., Ivanauskas, L., Bezruk, I., & Georgiyants, V. (2020). Effect of ecological factors on the accumulation of phenolic compounds in Iris species from Latvia, Lithuania and Ukraine. Phytochemical Analysis, 31(5), 545-563. https://doi.org/10.1002/pca.2918
  29. Orhan, I., Kartal, M., Tosun, F., & Şener, B. (2007). Screening of various phenolic acids and flavonoid derivatives for their anticholinesterase potential. Zeitschrift für Naturforschung C, 62(11-12), 829-832. https://doi.org/10.1515/znc-2007-11-1210
  30. Özcan, M. (1999). Antifungal effects of Micromeria myrtifolia boiss. & hohen. in boiss. and prangos uechtritzii boiss. hawsskn decoctions. Acta Alimentaria, 28(4), 355-360. https://doi.org/10.1556/aalim.28.1999.4.6
  31. Öztürk, M., Kolak, U., Topçu, G., Öksüz, S., & Choudhary, M.I. (2011). Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation. Food Chemistry, 126(1), 31-38. https://doi.org/10.1016/j.foodchem.2010.10.050
  32. Patel, D. K. (2022). Medicinal importance, pharmacological activities and analytical aspects of a flavonoid glycoside ‘Nicotiflorin’in the medicine. Drug Metabolism and Bioanalysis Letters Formerly: Drug Metabolism Letters, 15(1), 2-11. https://doi.org/10.2174/1872312815666220404110200
  33. Polat, R., & Satıl, F. (2012). An ethnobotanical survey of medicinal plants in Edremit Gulf (Balıkesir–Turkey). Journal of Ethnopharmacology, 139(2), 626-641. https://doi.org/10.1016/j.jep.2011.12.004
  34. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  35. Sadeq, O., Mechchate, H., Es-Safi, I., Bouhrim, M., Jawhari, F.Z., Ouassou, H.,. & Imtara, H. (2021). Phytochemical screening, antioxidant and antibacterial activities of pollen extracts from micromeria fruticosa, achillea fragrantissima, and phoenix dactylifera. Plants, 10(4), 676. https://doi.org/10.3390/plants10040676
  36. Salameh, N., Shraim, N., Jaradat, N., El Masri, M., Adwan, L., K’aibni, S.,. & AbuAlhasan, M. (2020). Screening of antioxidant and antimicrobial activity of Micromeria fruticosa serpyllifolia volatile oils: A comparative study of plants collected from different regions of west bank, Palestine. BioMaterials Research International, 2020(1), 4851879. https://doi.org/10.1155/2020/4851879
  37. Salim, M., & Necattin, T. (2018). A survey on wild plants with ethnobotanical use in the Bahçe and Hasanbeyli districts of Osmaniye, Turkey. GSC Biological and Pharmaceutical Sciences, 5(3). https://doi.org/10.30574/gscbps.2018.5.3.0133
  38. Šamec, D., Gruz, J., Durgo, K., Kremer, D., Kosalec, I., Valek Žulj, L.,. & Piljac-Žegarac, J. (2015). Molecular and cellular approach in the study of antioxidant/pro-oxidant properties of Micromeria croatica (Pers.) Schott. Natural Product Research, 29(18), 1770-1774. https://doi.org/10.1080/14786419.2014.999334
  39. Sargin, S.A. (2015). Ethnobotanical survey of medicinal plants in Bozyazı district of Mersin, Turkey. Journal of Ethnopharmacology, 173, 105-126. https://doi.org/10.1016/j.jep.2015.07.009
  40. Sargin, S.A., & Büyükcengiz, M. (2019). Plants used in ethnomedicinal practices in Gulnar district of Mersin, Turkey. Journal of Herbal Medicine, 15, 100224. https://doi.org/10.1016/j.hermed.2018.06.003
  41. Sarikurkcu, C., Andrade, J.C., Ozer, M.S., de Lima Silva, J.M.F., Ceylan, O., de Sousa, E.O., & Coutinho, H.D.M. (2020b). LC-MS/MS profiles and interrelationships between the enzyme inhibition activity, total phenolic content and antioxidant potential of Micromeria nervosa extracts. Food Chemistry, 328, 126930. https://doi.org/10.1016/j.foodchem.2020.126930
  42. Sarikurkcu, C., Hanine, H., Sarikurkcu, R.B., Sarikurkcu, R.T., & Amarowicz, R. (2020a). Micromeria myrtifolia: The influence of the extracting solvents on phenolic composition and biological activity. Industrial Crops and Products, 145, 111923. https://doi.org/10.1016/j.indcrop.2019.111923
  43. Schmidt, J.S., Lauridsen, M.B., Dragsted, L.O., Nielsen, J., & Staerk, D. (2012). Development of a bioassay-coupled HPLC-SPE-ttNMR platform for identification of α-glucosidase inhibitors in apple peel (Malus× domestica Borkh.). Food Chemistry, 135(3), 1692-1699. https://doi.org/10.1016/j.foodchem.2012.05.075
  44. Selvi, S., Polat, R., Çakilcioğlu, U., Celep, F., Dirmenci, T., & Ertuğ, Z.F. (2022). An ethnobotanical review on medicinal plants of the Lamiaceae family in Turkey. Turkish Journal of Botany, 46(4), 283-332. https://doi.org/10.55730/1300-008X.2712
  45. Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55. https://doi.org/10.5344/ajev.1977.28.1.49
  46. Taskın, T., Oksuz, M., Rayaman, E., Ermanoglu, M., Taskın, D., Acar, A.G.,. & Elcioglu, H.K. (2024). Phytochemical analysis and in vitro biological activity assessment of extracts from Micromeria myrtifolia. Journal of Environmental Health Research, 1-13. https://doi.org/0.1080/09603123.2024.2409829
  47. Vladimir-Knežević, S., Blažeković, B., Kindl, M., Vladić, J., Lower-Nedza, A. D., & Brantner, A. H. (2014). Acetylcholinesterase inhibitory, antioxidant and phytochemical properties of selected medicinal plants of the Lamiaceae family. Molecules, 19(1), 767-782. https://doi.org/10.3390/molecules19010767
  48. Vladimir-Knežević, S., Blažeković, B., Štefan, M.B., Alegro, A., Kőszegi, T., & Petrik, J. (2011). Antioxidant activities and polyphenolic contents of three selected Micromeria species from Croatia. Molecules, 16(2), 1454-1470. https://doi.org/10.3390/molecules16021454
  49. Weatherburn, M.W. (1967). Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry, 39: 971-974. https://doi.org/10.1021/ac60252a045
  50. Yesilada, E. (2005). Past and future contributions to traditional medicine in the health care system of the Middle-East. Journal of Ethnopharmacology, 100(1-2), 135-137. https://doi.org/10.1016/j.jep.2005.06.003
  51. Yeşilada, E. (2002). Biodiversity in Turkish folk medicine. In Biodiversity: biomolecular aspects of biodiversity and innovative utilization (pp. 119-135). Boston, MA: Springer US. https://doi.org/10.1007/978-1-4419-9242-0_14
  52. Yilmaz, M. A., Cakir, O., Zengin, G., Izol, E., & Behcet, L. (2024). The uprisal of a lost endemic edible species, Micromeria cymuligera: Comprehensive elucidation of its biological activities and phytochemical composition. Food Bioscience, 61, 104690. https://doi.org/10.1016/j.fbio.2024.104690
  53. Yilmaz, M.A, Ertas, A, Yener, I, Akdeniz, M, Cakir, O, Altun, M, Demirtas, I, Boga, M., Temel, H. (2018). A comprehensive LC-MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala. Journal of Pharmaceutical and Biomedical Analysis, 154, 413-424, https://doi.org/10.1016/j.jpba.2018.02.059
  54. Yu, S., Guo, Q., Jia, T., Zhang, X., Guo, D., Jia, Y.,. & Sun, J. (2021). Mechanism of action of nicotiflorin from Tricyrtis maculata in the treatment of acute myocardial infarction: From network pharmacology to experimental pharmacology. Drug design, development and therapy, 2179-2191. https://doi.org/10.2147/DDDT.S302617
  55. Bektaş, N.Y., Ersoy, E., Boğa, M., Boran, T., Çinar, E., Özhan, G.,. & Özkan, E.E. (2021). Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry, 45(5), 1621-1638. https://doi.org/10.3906/kim-2104-17
  56. Brahmi, F., Guendouze, N., Hauchard, D., Okusa, P., Kamagaju, L., Madani, K., & Duez, P. (2017). Phenolic profile and biological activities of Micromeria graeca (L.) Benth. ex Rchb. International Journal of Food Properties.20(sup2), 2070-2083. https://doi.org/10.1080/10942912.2017.1362650
  57. El Kamari, F., El Omari, H., El-Mouhdi, K., Chlouchi, A., Harmouzi, A., Lhilali, I.,. & Ousaaid, D. (2024). Effects of Different Solvents on the Total Phenol Content, Total Flavonoid Content, Antioxidant, and Antifungal Activities of Micromeria graeca L. from Middle Atlas of Morocco. Biochemistry Research International, 2024(1), 9027997. https://doi.org/10.1155/2024/9027997
  58. Eroglu Ozkan, E., Ersoy, E., Yesil Canturk, Y., Mataraci Kara, E., Cinar, E., Sahin, H.,. & Boga, M. (2022). The therapeutic potential of Ethnomedicinally important Anatolian Thyme species: a Phytochemical and Biological Assessment. Frontiers in Pharmacology, 13, 923063. https://doi.org/10.3389/fphar.2022.923063
  59. Ersoy, E., Karahan, S., Boğa, M., Çınar, E., İzgi, S., Kara, E. M.,. & Özkan, E. E. (2022). Evaluation of the medicinal potential of a traditionally important plant from Turkey: Cerinthe minor L. Istanbul Journal of Pharmacy, 52(1), 80-89. https://doi.org/10.26650/IstanbulJPharm.2022.1001685
  60. Ersoy, E., Ozkan, E.E., Karahan, S., Şahin, H., Cinar, E., Canturk, Y.Y.,. & Boga, M. (2023). Phytochemical analysis of essential oils and the extracts of an ethnomedicinal plant, Teucrium multicaule collected from two different locations with focus on their important biological activities. South African Journal of Botany, 153, 124-135. https://doi.org/10.1016/j.sajb.2022.12.020
  61. Mladenova, T., Stoyanov, P., Denev, P., Dimitrova, S., Katsarova, M., Teneva, D.,. & Bivolarska, A. (2021). Phytochemical Composition, Antioxidant and Antimicrobial Activity of the Balkan Endemic Micromeria frivaldszkyana (Degen) Velen. (Lamiaceae). Plants, 10(4), 710. https://doi.org/10.3390/plants10040710
  62. Sadeq, O., Mechchate, H., Es-Safi, I., Bouhrim, M., Jawhari, F.Z., Ouassou, H.,. & Imtara, H. (2021). Phytochemical screening, antioxidant and antibacterial activities of pollen extracts from micromeria fruticosa, achillea fragrantissima, and phoenix dactylifera. Plants, 10(4), 676. https://doi.org/10.3390/plants10040676
  63. Salameh, N., Shraim, N., Jaradat, N., El Masri, M., Adwan, L., K’aibni, S.,. & AbuAlhasan, M. (2020). Screening of antioxidant and antimicrobial activity of Micromeria fruticosa serpyllifolia volatile oils: A comparative study of plants collected from different regions of west bank, Palestine. BioMaterials Research International, 2020(1), 4851879. https://doi.org/10.1155/2020/4851879
  64. Šamec, D., Gruz, J., Durgo, K., Kremer, D., Kosalec, I., Valek Žulj, L.,. & Piljac-Žegarac, J. (2015). Molecular and cellular approach in the study of antioxidant/pro-oxidant properties of Micromeria croatica (Pers.) Schott. Natural Product Research, 29(18), 1770-1774. https://doi.org/10.1080/14786419.2014.999334
  65. Taskın, T., Oksuz, M., Rayaman, E., Ermanoglu, M., Taskın, D., Acar, A.G.,. & Elcioglu, H.K. (2024). Phytochemical analysis and in vitro biological activity assessment of extracts from Micromeria myrtifolia. Journal of Environmental Health Research, 1-13. https://doi.org/0.1080/09603123.2024.2409829
  66. Yu, S., Guo, Q., Jia, T., Zhang, X., Guo, D., Jia, Y.,. & Sun, J. (2021). Mechanism of action of nicotiflorin from Tricyrtis maculata in the treatment of acute myocardial infarction: From network pharmacology to experimental pharmacology. Drug design, development and therapy, 2179-2191. https://doi.org/10.2147/DDDT.S302617