Environmental Contaminants and Placental Transporters: Example of PCB vs. SLC and ABCB1

Objective & Hypothesis:Objective: Due to widespread presence and exposure to environmental pollutants, structural and functional changes in membrane transporters could occur, leading to plasental transport of these compounds.

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Key Findings & Data:In this study, the effects of PCBs on SLC abd ABCB1 membrane transport molecules were evaluated.

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Conclusion & Impact:In the bioinformatics findings, there were differences in 742 genes between PCB positive and negative samples in 55 samples that were sequenced (p

🔬Design:Research Article

📍Scope:60 samples

⚙️Variables:Plasenta · genomik · PCB

Abstract

Objective: Due to widespread presence and exposure to environmental pollutants, structural and functional changes in membrane transporters could occur, leading to plasental transport of these compounds. In this study, the effects of PCBs on SLC abd ABCB1 membrane transport molecules were evaluated. Materials and Methods: Hemochorial structured human placenta and endotheliochorial structured dog placenta were analyzed for 28 pollutants (PCB, PBDE, PAH and Organochlorines) using GC-MS. The expression profile of the placental whole genome were investigated with RNAseq, and in silico (molecular chelation) and in vitro (SLC and ABCB1 mRNA expression in the placental cell line HTR8/SVneo treated with PCB 101, PCB118) were evaluated. Results: PCB101 826.4μg/kg in one sample out of 60 samples tested; In 23 samples, PCB118 was found to be between 0.14 and 41.9μg/kg. In the bioinformatics findings, there were differences in 742 genes between PCB positive and negative samples in 55 samples that were sequenced (p

Özet

Amaç: Çevresel kirletici maruziyetine bağlı olarak işlevselliği değişen ve bozulan membran transportları nedeniyle, bu maddeler plasental bariyeri geçerek plasental kan dolaşımına geçebilmektedir. Çalışmada, çevresel kirleticilerin bu transport proteinleriyle etkileşimlerinin moleküler boyutta incelenmesi amaçlanmaktadır. Gereç ve Yöntem: Araştırma kapsamında hemokoryal yapıya sahip insan ve endotelyokoryal yapıya sahip köpek plasentasında; 28 kirleticinin analizi GC-MS ile yapılmış (PCB, PBDE, PAH ve Organik klorlu pestisitler); RNAseq ile plasental tüm genom ifade profili araştırılmış, in siliko (moleküler kenetleme) ve in vitro (PCB 101, PCB118 uygulanan plasental hücre hattı HTR8/SVneo’da SLC ve ABCB1 mRNA ifadesi) değerlendirilmiştir. Bulgular: Test edilen 60 örnek içerisinde bir örnekte PCB101 826.4μg/kg; 23 örnekte ise PCB118 0.14 ile 41,9μg/kg arasında bulundu. Biyoinformatik bulgularda sekans analizi yapılan 55 numunede PCB pozitif ve negatif numuneler arasında 742 gende farklılık bulundu (p

Keywords

Plasenta genomik PCB SLC ABCB1 Placenta genomic

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Cite This Article

DİKMEN, B. Y., UYAR, R., KUZUKIRAN, Ö., ÜNAL, M. A., ÇELİK, T., BOZTEPE, Ü. G., ALKAN, K. K., ÖZYÜNÜ, Ö., TURGUT, Y., SEVGİLİ, H. Ö., KANCA, H., AKTAN, Ç., FİLAZİ, A. (2022). Environmental Contaminants and Placental Transporters: Example of PCB vs. SLC and ABCB1. Balıkesir Sağlık Bilimleri Dergisi, Veteriner Farmakoloji Kongresi Özel Sayısı, 29–37. https://ajindex.com/articles/867328

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