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Journals / Ekin Journal of Crop Breeding and Genetics / 2022 / Cilt: 8 - Sayı: 2

In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)

Mehmet Burak TURGUT
Muhammet Çağrı OĞUZ
Burak ÖNOL
Cengiz SANCAK
Pages
101–107
Publication Date
2022-07-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotyl explant in an MS medium containing 2 mg/L TDZ.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Local populations and ecotypes are important genetic resources with genetic diversity.
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Key Findings & Data:It is possible to protect threatened genotypes and increase biodiversity with in vitro tissue culture techniques.
💡
Conclusion & Impact:According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotyl explant in an MS medium containing 2 mg/L TDZ.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:In vitro regeneration · callus induction · regeneration efficiency

Abstract

Local populations and ecotypes are important genetic resources with genetic diversity. It is possible to protect threatened genotypes and increase biodiversity with in vitro tissue culture techniques. Explant type is of great importance among the factors affecting the success of in vitro regeneration and callus induction. In this study, Sainfoin seeds belonging to the Gözlü ecotype were used as experiment material. MS media were prepared with TDZ and combinations of IBA-NAA. Callus induction and shoot regeneration rates of hypocotyl, leaf and cotyledon explants were determined in the sainfoin ecotype. According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotyl explant in an MS medium containing 2 mg/L TDZ. In the present study, important results were obtained that will contribute to the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity.

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Cite This Article

TURGUT, M. B., OĞUZ, M. Ç., ÖNOL, B., SANCAK, C. (2022). In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa). Ekin Journal of Crop Breeding and Genetics, Cilt: 8 - Sayı: 2, 101–107. https://ajindex.com/articles/753075

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References (3)

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