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Journals / Ekin Journal of Crop Breeding and Genetics / 2026 / Volume: 12 Issue: 2

Applications of Chromosome Doubling in Haploid Squashes and Pumpkins (Cucurbita spp.)

Emre İpek(Petektar Seed R&D Center)
Büşra Altuntaş(Petektar Seed R&D Center)
Süleyman Kavak(ISPARTA UNIVERSITY OF APPLIED SCIENCES, FACULTY OF AGRONOMICS AND AGRICULTURAL TECHNOLOGIES, DEPARTMENT OF AGRICULTURAL ECONOMICS, DEPARTMENT OF AGRICULTURE TECHNOLOGY)
Ş. Şebnem Ellialtioğlu(Doqutech Academy Llc. Co)
Pages
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Publication Date
2026-08-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, optimization studies on chromosome doubling in squash varieties are still needed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Squashes and pumpkins are important vegetable species belonging to the genus Cucurbita of the family Cucurbitaceae.
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Key Findings & Data:The genus Cucurbita (2n=40) comprises five cultivated species, the most economically important of which are Cucurbita pepo L., C. maxima Duch., and C. moschata Duch.
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Conclusion & Impact:However, optimization studies on chromosome doubling in squash varieties are still needed.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Squashes and pumpkins · haploid · Cucurbita pepo

Abstract

Squashes and pumpkins are important vegetable species belonging to the genus Cucurbita of the family Cucurbitaceae. The genus Cucurbita (2n=40) comprises five cultivated species, the most economically important of which are Cucurbita pepo L., C. maxima Duch., and C. moschata Duch. The doubled haploidization technique, which allows the rapid and efficient obtaining of pure lines in variety breeding programs in many plant species, is also successfully applied in the Cucurbita genus. The irradiated pollen technique yields successful results in all three squash species in obtaining haploid plants. Successful applications have been made in projects conducted at our company to obtain haploid plants in squash. However, it is not possible to propose a practical protocol for chromosome doubling in squash and obtaining doubled haploid (DH) plants from haploid plants. Although the literature contains successful applications and reports of pure-line seeds, the doubling step remains a key challenge in the production of pure lines in squash. For this purpose, studies were conducted on chromosome doubling in haploid squash plants under in vitro and in vivo conditions. Concentration and duration of colchicine applications were tested, and stomatal examinations revealed diploid plants. The most effective method was determined to be a 3-hour application period using a 0.5% colchicine dose used in acclimated young haploid seedlings at the 3-4 leaf stage. Plants grown from this treatment were self-pollinated to obtain pure-line seeds. However, optimization studies on chromosome doubling in squash varieties are still needed.

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Cite This Article

İpek, E., Altuntaş, B., Kavak, S., Ellialtioğlu, Ş. Ş. (2026). Applications of Chromosome Doubling in Haploid Squashes and Pumpkins (Cucurbita spp.). Ekin Journal of Crop Breeding and Genetics, Volume: 12 Issue: 2. https://ajindex.com/articles/1477717

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