Journals / Animal Health Production and Hygiene / 2019 / Cilt: 8 - Sayı: 2

Determination of Histamin Levels in the Fish Samples Obtained from Open Markets of Aydın

Aydın İli Pazar Yerlerinde Satışa Sunulan Balıklarda Bulunan Histamin Düzeylerinin Belirlenmesi

Pages
663–667
Publication Date
2019-06-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the minimum and maximum levels of histamine observed were 3,45 ppm and 30,32 ppm with the mean of 7,18 ppm. 26% of the histamine detected samples were in a range of 1 and 5 ppm, 70% of the positive samples were in the range of 5-10 ppm. %2 of the positive samples had histamine levels between 20-25 ppm and 30-35 ppm.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/Aim: Scombroid fish poisoning, is a syndrome resembling an allergic reaction that occurs after consuming fish contaminated with high levels of histamine prodeced in unhygienic conditions and stored improperly.
📊
Key Findings & Data:Material and Method: In this study, a total of 50 fish samples 15 bonito, 15 mackerel and 20 horse mackerel collected from open markets located in Aydın Province and histamine levels were determined by using ELISA technique.
💡
Conclusion & Impact:The results showed that the minimum and maximum levels of histamine observed were 3,45 ppm and 30,32 ppm with the mean of 7,18 ppm. 26% of the histamine detected samples were in a range of 1 and 5 ppm, 70% of the positive samples were in the range of 5-10 ppm. %2 of the positive samples had histamine levels between 20-25 ppm and 30-35 ppm.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Anahtar kelimeler: ELISA · histamin · Scombridae

Abstract

Background/Aim: Scombroid fish poisoning, is a syndrome resembling an allergic reaction that occurs after consuming fish contaminated with high levels of histamine prodeced in unhygienic conditions and stored improperly. It is caused by mainly consumption of Scomberesocidae and Scombridae fish such as tuna, mackerel, bonito. However, other fish apart from these families may also cause histamine fish poisoning. Poisoning caused by consumption of histamine, a biogenic amine, in fish meat derived from free histidine amino acid by decarboxylase enzymes of bacteria. This study was aimed to determine of histamin levels in the fish samples obtained from open markets of Aydın. Material and Method: In this study, a total of 50 fish samples 15 bonito, 15 mackerel and 20 horse mackerel collected from open markets located in Aydın Province and histamine levels were determined by using ELISA technique. Results and Conclusion: The presence of various levels of histamine was detected in all fish samples. The results showed that the minimum and maximum levels of histamine observed were 3,45 ppm and 30,32 ppm with the mean of 7,18 ppm. 26% of the histamine detected samples were in a range of 1 and 5 ppm, 70% of the positive samples were in the range of 5-10 ppm. %2 of the positive samples had histamine levels between 20-25 ppm and 30-35 ppm. None of the samples had higher limits than that Turkish Food Codex allowed 200 ppm .

Özet

Özbilgi/Amaç: Skombroid balık zehirlenmesi, yüksek seviyede histamin içeren ve iyi korunmamış balıkların tüketilmesiyle ortaya çıkan alerjik bir reaksiyondur. Histamin zehirlenmesi sıklıkla Scomberesocidae ve Scombridae familyalarına ait scombroid balıkları ton balığı, torik balığı, uskumru, lüfer ve zurna balığı ile ilişkilendirilmekle birlikte Skombroid ailesine mensup olmayan ama zehirlenmeye sebep olan farklı türde balıklardan da kaynaklanabilmektedir. Balık etinde bulunan serbest histidin aminoasidinin bakteriyel dekarboksilazlar ile histamine dönüştürülmesi zehirlenmenin temelini oluşturmaktadır. Bu çalışmada Aydın ili pazar yerlerinde satışa sunulan balıklarda bulunan histamin düzeylerinin belirlenmesi amaçlanmıştır. Materyal ve Metot: Çalışmada; Aydın ili açık pazarlarından toplanılan 50 adet balık 15 adet palamut balığı, 15 adet uskumru balığı ve 20 adet istavrit balığı örneğinde ELISA tekniği kullanılarak histamin miktarları araştırılmıştır. Bulgular ve Sonuç: İncelenen balık örneklerinin tamamında çeşitli düzeylerde histamin varlığı saptanmıştır. Örneklerdeki histamin düzeyi minimum 3,45 ppm, maksimum 30,32 ppm ve ortalama 7,18 ppm olarak belirlenmiştir. Ayrıca histamin düzeyinin örneklerden 13’ünde %26 1-5 ppm, 35’inde %70 5-10 ppm, 1’inde %2 20-25 ppm ve yine 1 örnekte de %2 30-35 ppm değerleri arasında olduğu tespit edilmiştir. İncelenen örneklerin hiçbirinde bulunan histamin seviyesinin Su Ürünleri Yönetmeliği tarafından belirtilen en yüksek değer olan 200 ppm’in üzerinde olmadığı belirlenmiştir.

🔖

Cite This Article

KIZANLIK, P. K., ŞAHİNER, C., GÖKSOY, E. Ö. (2019). Determination of Histamin Levels in the Fish Samples Obtained from Open Markets of Aydın. Animal Health Production and Hygiene, Cilt: 8 - Sayı: 2, 663–667. https://ajindex.com/articles/733959

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Ansdell V (2008). Food-borne illness. In: Keystone, J.S., Kozarsky, P.E., Freedman, D.O., Nothdurft, H.D., Connor, B.A. (Eds.), Travel Medicine, second ed. Mosby, Philadelphia, s 475-484.
  2. Bjornsdottir-Butler K, McCarthy SA, Dunlap PV, Benner RA (2016). Photobacterium angutsum and Photobacterium kishitanii, psychrotrophic high-level histamine-producing bacteria indigenous to tuna. Applied and Environmental Microbiology, 82:2167-2176.
  3. Cavanah DK, Casale TB (1993). Histamine. In: Kaliner MA, Metcalfe DD (eds.), The Mast Cell in Health and Disease, Bluefish-associated scombroid poisoning. New York, Basel, Hong Kong, s. 321–342.
  4. Chang SC, Kung HF, Chen HC, Lin CS, Tsai YH (2008). Determination of histamine and bacterial isolation in swordfish fillets (Xiphiasgladius) implicated in a foodborne poisoning. Food Control, 19:16-21.
  5. Chummun S, Neetoo H (2016). A study on the relationship between microbial growth, histamine development and organoleptic changes in retailed fresh sprangled emperor and bigeye tuna. Journal of Food Chemistry and Nanotechnology, 2:6-13.
  6. Edmunds WJ, Eitenmiller RR (1975). Effect of storage time and temperature on histamine content and histidine decarboxylase activity of aquatic species Journal of Food Science, 40:516-519. EFSA (European Food Safety Authority) (2017). Assessment of the incidents of histamine intoxication in some EU countries. EFSA Supporting Publications, 14(9):1301E. FDA (U.S. Food and Drug Administration) (2011). Fish and Fishery Products Hazards and Controls Guidance. Department of Health and Human Services Public Health Service, Food and Drug Administration Center For Food Safety and Applied Nutrition Office of Food Safety. FDA (2019). Chapter 7: Scombrotoxin (Histamine) Formation. https:// www.fda.gov ı media ı download. Son erişim 11.10.2019.
  7. Fernandez-Salguero J, Mackie M (1979). Histidine metabolism in mackerel (Scomber scombrus). Studies on histidine decarboxylase activity and histamine formatian during storage of flesh and liver under sterile and non- sterile conditions. Journal of Food Technology, 14:131-139.
  8. Flick GJ, Oria MP, Douglas L (2001). Potential hazards in cold-smoked fish: Biogenic amines. Special Supplement to the Journal of Food Science, 66(7):1088-1099. FSAI (Food Safety Authority of Ireland) (2019). Histamine in Fish and Fishery Products. https://www.fsai.ie ı faq ı histamine_fish. Son erişim 19.10.2019.
  9. Halstead B, Courville D (1967). Poisonous and venomous marine animals of the world. US. Governme