SavedAdd your journal

Journals / Sağlık Bilimleri Dergisi / 2022 / Cilt: 31 - Sayı: 2

REAL TIME ANALYSIS OF IMPEDANCE ALTERATIONS BY THE NEUROTOXICITY OF SCOPOLETIN ON SHSY5Y NEUROBLASTOMA CELLS

SHSY5Y NÖROBLASTOMA HÜCRELERİNDE SKOPOLETİN NÖROTOKSİSİTESİNE BAĞLI EMPEDANS DEĞİŞİKLİKLERİNİN GERÇEK ZAMANLI ANALİZİ

Ayşe Kübra KARABOĞA ARSLAN
Aysun ÖKÇESİZ
Leyla PAŞAYEVA
Pages
242–248
Publication Date
2022-08-11
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings from this study demonstrated that the growth inhibitory and cytotoxic effects of scopoletin on SHSY5Y cells may prove essential in the development of novel treatment regimens for neuroblastoma cancer.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Plant coumarins are important components of the human diet and a number of them are considered to possess chemopreventive and therapeutic properties against cancer.
📊
Key Findings & Data:In this study, the cytotoxic activity of scopoletin was studied against human neuroblastoma cells SHSY5Y.
💡
Conclusion & Impact:The findings from this study demonstrated that the growth inhibitory and cytotoxic effects of scopoletin on SHSY5Y cells may prove essential in the development of novel treatment regimens for neuroblastoma cancer.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sitotoksisite · skopoletin · SH-SY5Y

Abstract

Plant coumarins are important components of the human diet and a number of them are considered to possess chemopreventive and therapeutic properties against cancer. Scopoletin, a natural coumarin component has been shown to inhibit the growth of many cancer cells. In this study, the cytotoxic activity of scopoletin was studied against human neuroblastoma cells SHSY5Y. The activity of scopoletin was evaluated by Sulphorhodamine B (SRB) assay and xCELLigence system. It was demonstrated that scopoletin reduced human SHSY5Y neuroblastoma cell viability. Scopoletin inhibited the growth of the cell line and the IC50 values were 91.82 and 79.19 μM for 48 h and 72 h, respectively. The findings from this study demonstrated that the growth inhibitory and cytotoxic effects of scopoletin on SHSY5Y cells may prove essential in the development of novel treatment regimens for neuroblastoma cancer.

Özet

Bitki kumarinleri, insan diyetinin önemli bileşenleridir ve birçoğunun kansere karşı koruyucu ve tedavi edici özelliklere sahip olduğu bilinmektedir. Doğal bir kumarin bileşeni olan skopoletinin birçok kanser hücresinin büyümesini engellediği gösterilmiştir. Bu çalışmada, insan nöroblastom hücreleri SHSY5Y'ye karşı skopoletinin sitotoksik aktivitesi incelendi. Scopoletin aktivitesi, Sulphorhodamine B (SRB) testi ve xCELLigence sistemi ile değerlendirildi. Skopoletinin insan SHSY5Y nöroblastom hücre canlılığını azalttığı gösterildi. Scopoletin hücre büyümesini inhibe etti ve IC50 değerleri 48 ve 72 saat için sırasıyla 91,82 ve 79,19 µM’dir. Bu çalışmadan elde edilen bulgular, skopoletinin SHSY5Y hücreleri üzerindeki büyüme önleyici ve sitotoksik etkilerinin, nöroblastom kanseri için yeni tedavi rejimlerinin geliştirilmesinde önemli olabileceğini kanıtladı.

🔖

Cite This Article

ARSLAN, A. K. K., ÖKÇESİZ, A., PAŞAYEVA, L. (2022). REAL TIME ANALYSIS OF IMPEDANCE ALTERATIONS BY THE NEUROTOXICITY OF SCOPOLETIN ON SHSY5Y NEUROBLASTOMA CELLS. Sağlık Bilimleri Dergisi, Cilt: 31 - Sayı: 2, 242–248. https://ajindex.com/articles/697708

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Naz H, Tarique M, Khan P et al. Evidence of vanillin binding to CAMKIV explains the anti-cancer mechanism in human hepatic carcinoma and neuroblastoma cells. Mol Cell Biochem 2018; 438(1):35-45
  2. Giacoppo S, Iori R, Rollin P et al. Moringa isothiocyanate complexed with α-cyclodextrin: a new perspective in neuroblastoma treatment. BMC Complement Altern Med 2017; 17(1):1-10
  3. Jan SA, Shinwari ZK, Malik M et al. Antioxidant and anticancer activities of Brassica rapa: a review. MOJ Biol Med 2018; 3(4):175-178
  4. Yusefi M, Shameli K, Ali RR et al. Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract. J Mol Struct 2020; 1204:127539
  5. Velsankar K, Sudhahar S, Parvathy G et al. Effect of cytotoxicity and aAntibacterial activity of biosynthesis of ZnO hexagonal shaped nanoparticles by Echinochloa frumentacea grains extract as a reducing agent. Mater Chem Phys 2020; 239:121976
  6. Thakur A, Singla R, Jaitak V et al. Coumarins as anticancer agents: a review on synthetic strategies, mechanism of action and SAR studies. Eur J Med Chem 2015; 101:476-495
  7. Yuan C, Wang MH, Wang F et al. Network pharmacology and molecular docking reveal the mechanism of Scopoletin against non-small cell lung cancer. Life Sci 2021; 270:119105
  8. Bhattacharyya SS, Paul S, Mandal SK et al. A synthetic coumarin (4-methyl-7 hydroxy coumarin) has anti-cancer potentials against DMBA-induced skin cancer in mice. Eur J Pharmacol 2009; 614(1-3):128-136
  9. Tian Q, Wang L, Sun X et al. Scopoletin exerts anticancer effects on human cervical cancer cell lines by triggering apoptosis, cell cycle arrest, inhibition of cell invasion and PI3K/AKT signalling pathway. J Buon 2019; 24(3):997-1002
  10. Rahman MA, Hong JS, Huh SO et al. Antiproliferative properties of Saussurea lappa Clarke root extract in SH-SY5Y neuroblastoma cells via intrinsic apoptotic pathway. Anim Cells Syst 2015; 19(2):119-126
  11. Hua Y, Zhou N, Zhang Z et al. Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo. Int J Oncol 2021; 58(1):122-132
  12. Kumar A, Rocke JP, Kumar BN et al. Evolving treatments in high-risk neuroblastoma. Expert Opin Orphan Drugs 2020; 8(12):497-506
  13. Xie HR, Hu LS, Li G et al. SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson's disease. Chin Med J (Engl). 2010; 123:1086-1092
  14. RTCA SP Instrument Operator’s Manual. https://www.man