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Journals / MANTAR DERGİSİ / 2022 / Cilt: 13 - Sayı: 1

Comparing Cellulotic Enzyme Activities of Neocallimastix sp. in Orpin’s and Menke’s Media

Orpin ve Menke Besi Ortamlarında Neocallimastix sp'nin Selülotik Enzim Aktivitelerinin Karşılaştırılması

Altuğ KARAMAN
Halit YÜCEL
Kübra EKİNCİ
Sabahattin CÖMERTPAY
Pages
55–61
Publication Date
2022-04-28
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We also discovered that Menke’s media was better for the 5th (p

📌 3 Key Takeaways

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Objective & Hypothesis:Neocallimastix sp. is a member of the Anaerobic Gut Fungi (AGF) family with unique lignocellulotic enzymes, and it is usually grown in two basic media in vitro.
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Key Findings & Data:In this study, we measured the growth rate of Neocallimastix sp. in these two media up to 7 days and A similar progress was observed in both (p>0.05).
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Conclusion & Impact:We also discovered that Menke’s media was better for the 5th (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Anaerobik fungus · Enzim aktivitesi · Orpin

Abstract

Neocallimastix sp. is a member of the Anaerobic Gut Fungi (AGF) family with unique lignocellulotic enzymes, and it is usually grown in two basic media in vitro. Orpin’s mediais preferred for the detection of enzymatic activites while Menke’s is mostly chosen for evaluating the gases produced during the fermantation. Although these two media were shown to be effective for their targeted purposes, no attempt has been made to compare the activities of various cellulotic enzymes in them. In this study, we measured the growth rate of Neocallimastix sp. in these two media up to 7 days and A similar progress was observed in both (p>0.05). We also discovered that Menke’s media was better for the 5th (p

Özet

Neocallimastix sp., benzersiz lignoselülotik enzimlere sahip Anaerobik Gut Fungusları (AGF) ailesinin bir üyesidir ve genellikle in vitro olarak iki temel besi ortamında da yetiştirilir. Enzimatik aktivitelerin tespiti için Orpin besi ortamı tercih edilirken, fermantasyon sırasında oluşan gazların değerlendirilmesi için daha çok Menke besi ortamı tercih edilmektedir. Bu iki ortamın hedeflenen amaçlar için etkili olduğu gösterilmiş olmasına rağmen, bu besi ortamlarındaki çeşitli selülotik enzimlerin aktivitelerini karşılaştırmak için hiçbir girişimde bulunulmamıştır. Bu çalışmada Neocallimastix sp.'nin bu iki besiyerinde de 7 güne kadar büyüme hızı ölçülmüş ve her ikisinde de benzer bir gelişim gözlenmiştir (p>0.05). Ayrıca Menke besi ortamının Aviselaz’ın 5. (p

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Cite This Article

KARAMAN, A., YÜCEL, H., EKİNCİ, K., CÖMERTPAY, S. (2022). Comparing Cellulotic Enzyme Activities of Neocallimastix sp. in Orpin’s and Menke’s Media. MANTAR DERGİSİ, Cilt: 13 - Sayı: 1, 55–61. https://ajindex.com/articles/687630

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