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Journals / International Journal of Pure and Applied Sciences / 2026 / Volume: 12 Issue: 1

Antimicrobial properties of bacillus licheniformis from agricultural lands in Tunceli

Tunceli tarım arazilerinden elde edilen bacillus licheniformis'in antimikrobiyal özellikleri

Bülent Kar(MUNZUR UNIVERSITY)0000-0002-8839-2605
Özlem Araka(MUNZUR UNIVERSITY)0009-0004-4767-1204
Halit Yücel(MUNZUR UNIVERSITY)0000-0002-6196-5303
Ferit Can Yazdıç(MUNZUR UNIVERSITY)0000-0002-2762-3027
Altuğ Karaman(MUNZUR UNIVERSITY)0000-0003-4918-7796
Pages
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Publication Date
2026-06-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the findings of this study contribute to knowledge about the antimicrobial properties of Bacillus licheniformis.

📌 3 Key Takeaways

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Objective & Hypothesis:This study investigated the antimicrobial potential of Bacillus licheniformis strains isolated from agricultural soils in Tunceli, Turkey.
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Key Findings & Data:The isolates were successfully confirmed as Bacillus licheniformis by 16S rDNA sequencing and phylogenetic analysis.
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Conclusion & Impact:In conclusion, the findings of this study contribute to knowledge about the antimicrobial properties of Bacillus licheniformis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:soil bacteria · secondary metabolites · bacillus licheniformis

Abstract

This study investigated the antimicrobial potential of Bacillus licheniformis strains isolated from agricultural soils in Tunceli, Turkey. Soil samples were collected from minimally disturbed areas and bacterial strains were isolated using morphological, biochemical and molecular identification methods. The isolates were successfully confirmed as Bacillus licheniformis by 16S rDNA sequencing and phylogenetic analysis. The production of antimicrobial secondary metabolites was evaluated by culturing the isolates with different carbon sources (sucrose, glucose and fructose) and testing their activity against Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) using the disk diffusion method. The results determined that sucrose was the most effective carbon source to enhance antimicrobial activity, especially against Escherichia coli. Antibacterial activity generally increased with longer incubation times. This research highlights the potential of Bacillus licheniformis as a source of antibacterial substances. It offers an environmentally friendly alternative for its use in agricultural applications for biological control of pathogens. In conclusion, the findings of this study contribute to knowledge about the antimicrobial properties of Bacillus licheniformis. Understanding the factors affecting carbon source availability and metabolite production is important to exploit the antimicrobial potential of Bacillus licheniformis.

Özet

Bu çalışmada, Türkiye Tunceli'deki tarım topraklarından izole edilen Bacillus licheniformis suşlarının antimikrobiyal potansiyelleri araştırılmıştır. Toprak örnekleri minimal olarak bozulmuş alanlardan toplanmış ve bakteri suşları morfolojik, biyokimyasal ve moleküler tanımlama yöntemleri kullanılarak izole edilmiştir. İzolatlar, 16S rDNA dizilemesi ve filogenetik analiz yoluyla Bacillus licheniformis oldukları başarılı bir şekilde doğrulanmıştır. Antimikrobiyal sekonder metabolitlerin üretimi, izolatların farklı karbon kaynaklarıyla (sükroz, glikoz ve fruktoz) kültürlenmesi ve disk difüzyon yöntemi kullanılarak Escherichia coli (ATCC 25922) ve Staphylococcus aureus'a (ATCC 25923) karşı aktivitelerinin test edilmesiyle değerlendirilmiştir. Sonuçlar, özellikle Escherichia coli'ye karşı antimikrobiyal aktiviteyi artırmak için en etkili karbon kaynağının sükroz olduğu belirlenmiştir. Antibakteriyel aktivite genellikle daha uzun inkübasyon süreleriyle artmıştır. Bu araştırma, Bacillus licheniformis'in antibakteriyel madde kaynağı olarak potansiyelini vurgulamaktadır. Patojenlerin biyolojik kontrolü için tarımdaki uygulamalarda kullanılması için çevre dostu bir alternatif sunmaktadır. Sonuç olarak, bu çalışmanın bulguları Bacillus licheniformis'in antimikrobiyal özellikleri hakkında bilgilere katkıda bulunmaktadır. Karbon kaynağındaki kullanılabilirliği ve metabolit üretimini etkileyen faktörleri anlamak, Bacillus licheniformis'in antimikrobiyal potansiyelinden yararlanmak için önemlidir.

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Cite This Article

Kar, B., Araka, Ö., Yücel, H., Yazdıç, F. C., Karaman, A. (2026). Antimicrobial properties of bacillus licheniformis from agricultural lands in Tunceli. International Journal of Pure and Applied Sciences, Volume: 12 Issue: 1. https://doi.org/10.29132/ijpas.1804127

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