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Journals / Türk Nefroloji Diyaliz ve Transplantasyon Dergisi / 2018 / Cilt: 27 - Sayı: 1

The Effects of Doxycycline on Peritoneal Fibrosis Induced by Chemical Peritonitis in Rats

Sıçanlarda Doksisiklinin Kimyasal Peritonite Bağlı Peritoneal Fibrozis Gelişimi Üzerine Etkisinin İncelenmesi

Serkan YILDIZ
Zahide ÇAVDAR
Taner ÇAMSARI
Sülen SARIOĞLU
Ensari GÜNELİ
Gülgün OKTAY
Osman YILMAZ
Pages
49–56
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

RESULTS: Immature collagen levels in parietal peritoneum, parietal-visceral peritoneal thickness and inflammation scores were compared and a significant difference was observed between the groups (p

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Objective & Hypothesis:OBJECTIVE: We investigated the effects of doxycycline on changes in peritoneal morphology, matrix metalloproteinase (MMP) and tissue inhibitor of matrix metalloproteinase (TIMP) activities in peritoneal lavage fluids in a rat model of peritoneal fibrosis due to chemical peritonitis.
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Key Findings & Data:MATERIAL and METHODS: 21 rats were divided into three groups (n=7).
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Conclusion & Impact:RESULTS: Immature collagen levels in parietal peritoneum, parietal-visceral peritoneal thickness and inflammation scores were compared and a significant difference was observed between the groups (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: We investigated the effects of doxycycline on changes in peritoneal morphology, matrix metalloproteinase (MMP) and tissue inhibitor of matrix metalloproteinase (TIMP) activities in peritoneal lavage fluids in a rat model of peritoneal fibrosis due to chemical peritonitis. MATERIAL and METHODS: 21 rats were divided into three groups (n=7). In the first group, we administered chlorhexidine gluconate (CHX) intraperitoneally (i.p.) to the rats. In the second group, we administered i.p. doxycycline in addition to CHX to rats. The third group was the control. MMP-2, MMP-9, TIMP-1, TIMP-2 and procollagen type I C-terminal peptide (PICP) levels in the peritoneal fluid were analyzed. Thickness of the peritoneum, amount of immature collagen and the inflammation scores were measured in peritoneal tissue samples. RESULTS: Immature collagen levels in parietal peritoneum, parietal-visceral peritoneal thickness and inflammation scores were compared and a significant difference was observed between the groups (p

Özet

AMAÇ: Çalışmada; sıçanlarda kimyasal peritonite bağlı peritoneal fibrozis modelinde, doksisiklinuygulamasının periton morfolojisindeki değişiklikler, periton yıkama sıvısındaki matriks metalloproteaz(MMP) ve matriks metalloproteaz doku inhibitörü (TIMP) aktiviteleri üzerine etkilerini incelemeyiamaçladık.GEREÇ ve YÖNTEMLER: Çalışmaya alınan 21 adet sıçan her grupta yedi adet olacak şekildeüç gruba ayrıldı. Birinci gruptaki sıçanlarda klorhekzidin glukonat (CHX) ile kimyasal peritonitoluşturuldu, ikinci grupta CHX ile kimyasal peritonit oluşturulan sıçanlara intraperitoneal doksisiklinverildi, üçüncü grup ise kontrol grubu olarak kabul edildi. Çalışma sonunda sakrifiye edilen sıçanlardanperiton yıkama sıvıları, parietal ve visseral periton doku örnekleri alındı. Peritoneal yıkama sıvılarındaMMP-2, MMP-9, TIMP-1, TIMP-2 ve prokollajen tip I C-terminal peptid (PIPC) düzeyleri ölçümüyapıldı. Periton doku örneklerinde kalınlık ölçümü, olgunlaşmamış kollajen düzeyi ölçümü veinflamasyon skorlaması yapıldı.BULGULAR: Parietal periton olgunlaşmamış kollajen düzeyleri, parietal-visseral periton kalınlıklarıve inflamasyon skorları açısından gruplar arasında anlamlı fark saptandı (p

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Cite This Article

YILDIZ, S., ÇAVDAR, Z., ÇAMSARI, T., SARIOĞLU, S., GÜNELİ, E., OKTAY, G., YILMAZ, O. (2022). The Effects of Doxycycline on Peritoneal Fibrosis Induced by Chemical Peritonitis in Rats. Türk Nefroloji Diyaliz ve Transplantasyon Dergisi, Cilt: 27 - Sayı: 1, 49–56. https://ajindex.com/articles/636078

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References (14)

  1. Flessner MF: Peritoneal transport physiology: Insights from basic research. J Am Soc Nephrol 1991
  2. 135 2. Dobbie JW: Durability of the peritoneal membrane. Perit Dial Int 1995
  3. 92 3. Dobbie JW: Pathogenesis of peritoneal fibrosing syndromes (sclerosing peritonitis) in peritoneal dialysis. Perit Dial Int 1992
  4. 27 4. Fukudome K, Fujimoto S, Sato Y, Hisanaga S, Eto T: Peritonitis increases MMP-9 activity in peritoneal effluent from CAPD patients. Nephron 2001
  5. 41 5. Bode W, Maskos K: Structural basis of the matrix metalloproteinases and their physiological inhibitors, the tissue inhibitors of metalloproteinases. Biol Chem 2003
  6. 872 6. Kossakowska AE, Edwards DR, Lee SS, Urbanski LS, Stabbler AL, Zhang CL, Phillips BW, Zhang Y, Urbanski SJ: Altered balance between matrix metalloproteinases and their inhibitors in experimental biliary fibrosis. Am J Pathol 1998
  7. 1902 7. Martin J, Yung S, Robson RL, Steadman R, Davies M: Production and regulation of matrix metalloproteinases and their inhibitors by human peritoneal mesothelial cells. Perit Dial Int 2000
  8. 533 8. Hanemaaijer R, Visser H, Koolwijk P, Sorsa T, Salo T, Golub LM, van Hinsbergh VW: Inhibition of MMP synthesis by doxycycline and chemically modified tetracyclines (CMTs) in human endothelial cells. Adv Dent Res 1998
  9. 118 9. Lalu MM, Gao CQ, Schulz R: Matrix metalloproteinase inhibitors attenuate endotoxemia induced cardiac dysfunction: A potential role for MMP-9. Mol Cell Biochem 2003
  10. 66 10. Clark JD, Gebhart GF, Gonder JC, Keeling ME, Kohn DF: Special Report: The 1996 Guide for the Care and Use of Laboratory Animals. ILAR J 1997
  11. 48 11. Hirahara I, Ogawa Y, Kusano E, Asano Y: Activation of matrix metalloproteinase-2 causes peritoneal injury during peritoneal dialysis in rats. Nephrol Dial Transplant 2004
  12. 1741 12. Lalu MM, Gao CQ, Schulz R: Matrix metalloproteinase inhibitors attenuate endotoxemia induced cardiac dysfunction: A potential role for MMP-9. Mol Cell Biochem 2003
  13. 66 13. Ghiselli R, Giacometti A, Cirioni O, Mocchegiani F, Orlando F, Del Prete M, D’Amato G, Scalise G, Saba V: Quinupristin/dalfopristin bonding in combination with intraperitoneal antibiotics prevent infection of knitted polyester graft material in a subcutaneous rat pouch model infected with resistant Staphylococcus epidermidis. Eur J Vasc Endovasc Surg 2002
  14. 234 14. Wu CM, Lin MW, Cheng JT, Wang YM, Huang YW, Sun WZ, Lin CR: Regulated, electroporat