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Journals / Türk Jinekoloji ve Obstetrik Derneği Dergisi / 2008 / Cilt: 5 - Sayı: 1

Evaluation of VEGF, EGF-R AND TGF-α in placenta of pregnancies with preeclampsia by immunohistochemistry

Preeklampsi hastalarının plasentalarında immünohistokimya metodu ile VEGF, EGF-R VE TGF-α bakışı

Hasan Tayfun ÖZÇAKIR
Sermet SAĞOL
Fuat AKERCAN
Nedim KARADADAŞ
Mustafa Coşan TEREK
Gülşen GİRAY
Teksin ÇIRPANLI
Pages
40–45
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclus ion: Immunos taining of VEGF, EGF-R and TGF- α is signif icantly higher i n plac enta biopsi es in patie nts with preeclampsia.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVE: The ai m of the study is to determine VEGF, EGFR and TGF- α with the immunohistochemical staining in placenta biopsies of patients with preeclampsia.
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Key Findings & Data:RESULTS: The VEGF, EGFR and TGF- α expression was significantly higher in placenta biopsies of preeclamptic patient compared to controls (271.2±22.65 versus 201.9±12.33, p=0.000; 186.3±4.98 versus 150.3±5.7, p=0.000; 185.1±7.48 versus 169.2±6.19, p=0.000; respectively).
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Conclusion & Impact:Conclus ion: Immunos taining of VEGF, EGF-R and TGF- α is signif icantly higher i n plac enta biopsi es in patie nts with preeclampsia.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: The ai m of the study is to determine VEGF, EGFR and TGF- α with the immunohistochemical staining in placenta biopsies of patients with preeclampsia. Design: Prospective case control study. MATERIALS-METHODS: Biopsies from the placenta were obtained from ten patients with preeclapmsia and ten patients of control group during the cesarean section. Placenta biopsies were examined for VEGF, EGF-R and TGF- α distribution with avidin-biotinperoxidase immunohistochemistry. The staining intensities were graded semi-quantitatively by the two histologists who were blinded to the groups and the H-score was calculated by light microscopic examination. RESULTS: The VEGF, EGFR and TGF- α expression was significantly higher in placenta biopsies of preeclamptic patient compared to controls (271.2±22.65 versus 201.9±12.33, p=0.000; 186.3±4.98 versus 150.3±5.7, p=0.000; 185.1±7.48 versus 169.2±6.19, p=0.000; respectively). Conclus ion: Immunos taining of VEGF, EGF-R and TGF- α is signif icantly higher i n plac enta biopsi es in patie nts with preeclampsia.

Özet

AMAÇ: Preekl amptik hastaların plasentalarında, immünohistokimyasal boyama yöntemi ile, vasküler endotelial büyüme faktörü (VEGF), ep idermal büyüme faktörü recept örü (EGF-R) ve transforming büyüme faktörü alfa’nın (TGF-α) araştırılması. Planlama: Prospektif vaka kontrol çalışması. GEREÇ-YÖNTEM: Çalışma grubuna 10 preeklamptik vaka ve kontrol grubuna da 10 normal gebelik vakası dahil edildi. Vakalardan sezaryen doğum esnasında plasenta biyopsileri alındı. Biyopsi örneklerinde, avidin-biotin-peroksidaz immünohistokimyasal yöntem ile ışık mikroskopu altında, VEGF, EGF-R ve TGF-α dağılımı araştırıldı. Boyanma yoğunluğu semikantitatif olarak derecelendirildi ve grupları bilmeyen 2 histolog tarafından H-skorları hesaplandı. SONUÇLAR: Preekl amptik hastaların plasentalarında VEGF, EGF-R ve TGF-α ekspresyonu anlamlı olarak daha yüksek bulundu (sırasıyla; 271.2±22.65 versus 201.9±12.33, p=0.000; 186.3±4.98 versus 150.3±5.7, p=0.000; 185.1±7.48 versus 169.2±6.19,p=0.000). YORUM: Preeklamptik hastaların plasentalarında VEGF, EGF-R ve TGF-Éø ekspresyonu anlamlı olarak yüksekdir.

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Cite This Article

ÖZÇAKIR, H. T., SAĞOL, S., AKERCAN, F., KARADADAŞ, N., TEREK, M. C., GİRAY, G., ÇIRPANLI, T. (2022). Evaluation of VEGF, EGF-R AND TGF-α in placenta of pregnancies with preeclampsia by immunohistochemistry. Türk Jinekoloji ve Obstetrik Derneği Dergisi, Cilt: 5 - Sayı: 1, 40–45. https://ajindex.com/articles/631700

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References (15)

  1. Driul L, Damante G, D'Elia A, Springolo F, Ianni A, Di Leonardo C, Angelini M, Marchesoni D. Screening for pre-eclampsia in a low-risk population at 24 weeks: uterine artery Doppler flow velocimetry and genetic variants of factor V, prothrombin and methylenetetrahydrofolate reductase. Minerva Ginecol. 2004; 56: 385- 90
  2. Levine RJ, Karumanchi SA.Circulating angiogenic factors in preeclampsia. Clin Obstet Gynecol. 2005; 48: 372- 86
  3. Torry DS, Hinrichs M, Torry RJ. Determinants of placental vascularity: Review. Am J Reprod Immunol. 2004; 51: 257-68
  4. Kingdom JC, Kaufmann P. Oxygen and placental vascular development. Adv Exp Med Biol. 1999; 474: 259- 75
  5. Trollmann R, Amann K, Schoof E, Beinder E, Wenzel D, Rascher W, Dotsch J. Hypoxia activates the human placental vascular endothelial growth factor system in vitro and in vivo. Am J Obstet Gynecol. 2003; 188: 517- 23
  6. Tsatsaris V, Goffin F, Munaut C, Brichant JF, Pignon MR, Noel A, Schaaps JP, Cabrol D, Frankenne F, Foidart JM. Overexpression of the soluble vascular endothelial growth factor receptor in preeclamptic patients: pathophysiological consequences. Clin Endocrinol Metab. 2003; 88: 5555- 63
  7. Favier J, Corvol P. Physiological angiogenesis. Therapie 2001;56: 455- 63
  8. Shore VH, Wang TH, Wang CL, Torry RJ, Caudle MR, Torry DS. Vascular endothelial growth factor, placenta growth factor and their receptors in isolated human trophoblast. Placenta 1997; 18: 657- 65
  9. Eleonora Sgambati, Mirca Marini, Giorgia D. Zappoli Thyrion, Elena Parretti, Giorgio Mello, Claudio Orlando, Lisa Simi, Carmela Tricarico, Gherardo Gheri, Enzo Brizzi. VEGF expression in the placenta from pregnancies complicated by hypertensive disorders. BJOG 2004;111: 564- 70
  10. Khong TY, Liddell HS, Robertson WB. Defective haemochorial placentation as a cause of miscariage. Br J Obstet Gynaecol 1987; 94: 649- 55
  11. Poston L. Maternal vascular function in pregnancy. J Hum Hypertens 1996; 10: 391- 4
  12. Zhou Y, Damsky CH, Fisher SJ. Preeclampsia is associated with failure of human cytotrophoblast to mimic a vascular adhesion phenotype: one cause of defective endovascular invasion in this syndrome? J Clin Invest 1997; 99: 2152- 64
  13. Damsky CH, Fisher SJ. Trophoblast pseudo-vasculogenesis: faking it with endothelial adhesion receptors. Curr Opin Cell Biol 1998; 10: 660- 6
  14. Roberts JM, Redman CWG. Preeclampsia: more than pregnancyinduced hypertension. Lancet 1993; 341: 1447- 51
  15. Ferrara N. Vascular endothelia