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Journals / Türk Biyokimya Dergisi / 2017 / Cilt: 42 - Sayı: 3

Deneysel iskemi/reperfüzyon modelinde apoptotik hücre ölümü için kritik zamanlama ve N-asetilsistein’in etkisi

Critical time point for apoptotic cell death in an experimental ischemia/reperfusion model and the effect of N-acetylcystein

Devrim DEMİR-DORA
Pages
265–272
Publication Date
2022-11-03
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Tissues were examined immunohistochemically at 0, 1 h, 24 h, 5 days and 10 days for detection of apoptotic cells Results: Our results showed that an ischemia for 60 min followed by reperfusion for 60 min triggered apoptosis.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Kidney transplantation is an important treatment option in end stage renal failure.
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Key Findings & Data:Tissues were examined immunohistochemically at 0, 1 h, 24 h, 5 days and 10 days for detection of apoptotic cells Results: Our results showed that an ischemia for 60 min followed by reperfusion for 60 min triggered apoptosis.
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Conclusion & Impact:Moreover, N-acetylcystein significantly diminished both the ischemia/reperfusion damage and apoptosis.Conclusion: We anticipate our results would be important for kidney transplantation in estimating the critical time point for apoptosis and administration of N-acetylcysteinprior to removal of the organ may be important in delaying the onset of apoptosis.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Kidney transplantation is an important treatment option in end stage renal failure. Tissue death may be an important problem when a kidney is removed from a cadaver and transported to a donor a long distance away. The purpose of this study is to determine the critical time point for apoptotic cell death in a renal ischemia/reperfusion model and determine the effects of N-acetylcystein on apoptosis induced by ischemia injury.Methods: Apoptotic cell death after induced renal ischemia followed by reperfusion, was estimated in a group of Wistar albino rats by immunoflourescence and ELISA techniques. N-acetylcystein, an antioxidant agent, was given to the rats to study the effect on apoptosis. Tissues were examined immunohistochemically at 0, 1 h, 24 h, 5 days and 10 days for detection of apoptotic cells Results: Our results showed that an ischemia for 60 min followed by reperfusion for 60 min triggered apoptosis. Moreover, N-acetylcystein significantly diminished both the ischemia/reperfusion damage and apoptosis.Conclusion: We anticipate our results would be important for kidney transplantation in estimating the critical time point for apoptosis and administration of N-acetylcysteinprior to removal of the organ may be important in delaying the onset of apoptosis.

Özet

Amaç: Böbrek nakli son dönem kronik böbrek yetmezliğinde önemli bir tedavi seçeneğidir. Kadavradan böbrek çıkarılıp donöre takılmak üzere uzun mesafelere nakledildiğinde doku ölümü önemli bir sorun olabilir. Bu çalışmanın amacı renal iskemi reperfüzyon modelinde apoptotik hücre ölümü için kritik zaman aralığını saptamak ve N-asetilsistein'in iskemik hasara bağlı olarak indüklenen apoptoz üzerindeki koruyucu etkisini göstermektir.Yöntem: Renal iskemi-reperfüzyon sonrası apoptotik hücre ölümü Wistar albino sıçanlarda immünofloresans ve ELISA yöntemleriyle gösterilmiştir. Deneklere apoptoz üzerindeki etkisinin gösterilmesi amacıyla bir antioksidan ajan olan N-asetilsistein verilmiştir. Dokular apoptotik hücrelerin gösterilmesi için 0, 1. saat, 24. saat, 5. gün ve 10. günlerde immünohistokimyasal olarak incelenmiştir.Bulgular: Sonuçlarımız 60 dakikalık iskemiyi takiben 60 dakikalık reperfüzyon yapılmasının apoptozu tetiklediğini göstermektedir. Bunun dışında N-asetilsistein hem iskemi-reperfüzyon hasarını hem de apoptozu anlamlı olarak azaltmıştır.Sonuç: Bu çalışmadan elde edilen sonuçların, organ transplantasyonunda apoptoz için kritik zaman sınırının belirlenmesi ve böbreğin çıkartılmasından önce N-asetilsistein verilmesinin apoptozun gelişimini geciktirebildiğini göstermesi açısından önemli olacağını düşünmekteyiz

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Cite This Article

DEMİR-DORA, D. (2022). Deneysel iskemi/reperfüzyon modelinde apoptotik hücre ölümü için kritik zamanlama ve N-asetilsistein’in etkisi. Türk Biyokimya Dergisi, Cilt: 42 - Sayı: 3, 265–272. https://ajindex.com/articles/621313

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