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Journals / Türk Biyokimya Dergisi / 2014 / Cilt: 39 - Sayı: 3

Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Farklı karbon kaynakları üzerinde büyüyen Yarrowia lipolytica suşları tarafından sitrik ve izositrik asidin üretimi

Cengiz ÇORBACI
Gönül ÇELİK
Onur AKPINAR
Füsun Bahriye UÇAR
Pages
285–290
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, in order to meet this need, further studies which will yield to the maximum citric acid production should be performed by utilizing waste carbon sources and by using new low-cost but high citric acid-producing strains

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The goal of this study was to determine the influence of various carbon sources on the citric and isocitric acid production by various Yarrowia lipolytica strains.
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Key Findings & Data:The amount of citric and isocitric acid production was determined via enzymatic reactions.Results: In this study, 22 Y. lipolytica strains were investigated for the organic acid production.
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Conclusion & Impact:Therefore, in order to meet this need, further studies which will yield to the maximum citric acid production should be performed by utilizing waste carbon sources and by using new low-cost but high citric acid-producing strains
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The goal of this study was to determine the influence of various carbon sources on the citric and isocitric acid production by various Yarrowia lipolytica strains. Methods: The yeasts used in our study were first investigated for organic acid production using screening media. Then, the effect of several complex carbon sources on the citric and isocitric acid production of selected yeast strains was investigated. The amount of citric and isocitric acid production was determined via enzymatic reactions.Results: In this study, 22 Y. lipolytica strains were investigated for the organic acid production. Among these strains, 2 strains (TEM YL 3 and TEM YL 20) were found to be highest organicacid producer. Taken all results together, the highest amounts of citric acid (66.2 g/L for TEM YL 3, 50.0 g/L for TEM YL 20) were observed in the production medium containing sunflower oil. Conclusion: Citric acid consumption, and thus, the need for it are constantly rising in our country, which imports citric acid. Therefore, in order to meet this need, further studies which will yield to the maximum citric acid production should be performed by utilizing waste carbon sources and by using new low-cost but high citric acid-producing strains

Özet

Amaç: Bu çalışmada, Yarrowia lipolytica suşları tarafından sitrik ve izositrik asit üretimi üzerine çeşitli karbon kaynaklarının etkisinin saptanması amaçlanmıştır.Metod: İlk olarak çalışmamızda kullandığımız mayalar, organik asit üretimleri açısından tarama besiyerlerinde test edilmişlerdir. Daha sonra, bu maya strainleri tarafından üretilen sitrik ve izositrik asit üretimi üzerine farklı karbon kaynaklarının etkisi araştırılmıştır. Sitrik ve izositrik asit üretim miktarları enzimatik test kitleri kullanılarak saptanmıştır.Bulgular: Bu çalışmada, organik asit üretimi açısından 22 Y. lipolytica suşu incelenmiştir. Bu 22 maya suşu arasından iki suşun (TEM YL 3 ve TEM YL 20), en yüksek organik asit üretici maya suşları olduğu bulunmuştur. Bütün sonuçlar değerlendirildiğinde, en yüksek sitrik asit üretimi (TEM YL 3 için 66.2 g/L, TEM YL 20 için 50.0 g/L) ayçiçek yağı içeren üretim ortamında saptanmıştır.Sonuç: Özellikle sitrik asit ithalatı yapılan ülkemizde, sitrik asit tüketimi ve ona olan ihtiyaç sürekli olarak artmaktadır. Atık karbon kaynaklarının değerlendirilmesi ve maliyeti düşük ancak verimi yüksek sitrik asit üreten yeni suşların kullanılarak, maksimum sitrik asit verimini sağlayacak daha ileri çalışmalara ihtiyaç duyulmaktadır

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Cite This Article

ÇORBACI, C., ÇELİK, G., AKPINAR, O., UÇAR, F. B. (2022). Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources. Türk Biyokimya Dergisi, Cilt: 39 - Sayı: 3, 285–290. https://ajindex.com/articles/621185

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