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Journals / Türk Biyokimya Dergisi / 2014 / Cilt: 39 - Sayı: 3

Production and partial purification of extracellular lipase produced by a novel yeast strain Candida odintsovae TY42

[Yeni bir maya suşu olan Candida odintsovae TY42 tarafından üretilen hücre dışı lipazın üretimi ve kısmi saflaştırılması]

Cengiz ÇORBACI
Funda KARTAL
Hüsniye Tansel YALÇIN
Pages
351–356
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The molecular weight of the purified lipase was estimated to be about 39 kDa.Conclusion: This paper is the first study representing lipase production by C. odintsovae

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Nowadays, in most of industrial technology, especially biotechnological processes, use of enzymes is increasingly widespread.
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Key Findings & Data:Therefore, in this research, we aimed to isolate and characterize lipase producing yeasts and purify the extracellular lipase produced by a novel yeast strain Candida odintsovae TY42.Methods: The yeasts used in the study were isolated from olive oils and identified based on sequence analyses of ITS1-5.8S rRNA-ITS2 and D1/D2 domain of 26S rRNA.
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Conclusion & Impact:The molecular weight of the purified lipase was estimated to be about 39 kDa.Conclusion: This paper is the first study representing lipase production by C. odintsovae
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Nowadays, in most of industrial technology, especially biotechnological processes, use of enzymes is increasingly widespread. With respect to discovery of new enzymes, one of the most successful methods is isolation of microorganisms from natural habitats. Therefore, in this research, we aimed to isolate and characterize lipase producing yeasts and purify the extracellular lipase produced by a novel yeast strain Candida odintsovae TY42.Methods: The yeasts used in the study were isolated from olive oils and identified based on sequence analyses of ITS1-5.8S rRNA-ITS2 and D1/D2 domain of 26S rRNA. The optimal nutritional and physicochemical conditions were determined by investigating the effects of initial pH and temperature and different carbon sources on enzyme production in basal medium. The lipase was purified by ammonium sulfate and acetone precipitation and anion exchange chromatography.Results: Three lipase producing yeast isolates were obtained from olive oils produced in Aegean region. The sequencing of rRNA regions revealed that novel strains, TY17, TY42 and TY54 identified as Candida odintsovae. C. odintsovae TY42 showed higher lipase activity and therefore, the yeast was selected for further investigations. The maximum lipase production was achieved in a medium containing fish oil after 144 h of incubation. The lipase was purified 12.8 fold. The molecular weight of the purified lipase was estimated to be about 39 kDa.Conclusion: This paper is the first study representing lipase production by C. odintsovae

Özet

Amaç: Günümüzde, endüstriyel teknolojilerin çoğunda, özellikle de biyoteknolojik işlemlerde enzimlerin kullanımı giderek yaygınlaşmaktadır. Yeni enzimlerin keşfi ile ilgili olarak, en başarılı metotlardan bir tanesi, doğal habitatlardan mikroorganizmaların izolasyonudur. Bu yüzden bu araştırmada, lipaz üretici mayaların izolasyonu ve karakterizasyonu ve yeni bir maya suşu olan Candida odintsovae TY42 tarafından üretilen hücre dışı lipazın saflaştırılması amaçlanmıştır.Metod: Bu çalışmada kullanılan mayalar, zeytinyağlarından izole edilmiş ve ITS1-5.8S rRNAITS2 ve 26S rRNA D1/D2 domaininin dizi analizine göre tanımlanmıştır. Optimal besinsel ve fizikokimyasal koşullar, başlangıç pH, sıcaklık ve farklı karbon kaynaklarının bazal ortamda enzim üretimi üzerine etkileri incelenerek saptanmıştır. Lipaz, amonyum sülfat ve aseton çöktürmesi ve anyon değişim kromatografisi ile saflaştırılmıştır.Bulgular: Lipaz üretici maya izolatları, Ege bölgesinde üretilen zeytinyağlarından elde edilmiştir. rRNA bölgelerinin dizi analizi, bu yeni suşların, TY17, TY42 ve TY54, Candida odintsovae'ye dahil olduğunu ortaya çıkarmıştır. C. odintsovae TY42, en yüksek lipaz aktivitesi göstermiş ve bu yüzden, bu maya ileriki çalışmalar için seçilmiştir. Maksimum lipaz üretimi, balık yağı içeren ortamda inkübasyonun 144. saatinden sonra elde edilmiştir. Lipaz, 12.8 kat saflaştırılmıştır. Saflaştırılan lipazın molekül ağırlığı yaklaşık olarak 39 kDa olarak hesaplanmıştır.Sonuç: Bu makale, C. odintsovae tarafından lipaz üretimini gösteren ilk çalışmadır

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Cite This Article

ÇORBACI, C., KARTAL, F., YALÇIN, H. T. (2022). Production and partial purification of extracellular lipase produced by a novel yeast strain Candida odintsovae TY42. Türk Biyokimya Dergisi, Cilt: 39 - Sayı: 3, 351–356. https://ajindex.com/articles/621179

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