Septik ratların böbrek dokularında serbest radikal metabolizmasına vitamin D'nin etkisi

Conclusion: Vitamin D treatment does not seem to have protective role against renal toxicity in sepsis.Nutrition with vitamin D in sepsis may have suppressive effect on antioxidant enzymes sush as CAT and GST due to reduced substrat level which use hydrogen peroxide (H2 O2 ) and GSH as a substrate

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Sepsis is a common cause of morbidity and mortality in the intensive care unit.

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Key Findings & Data:Studies on the antioxidant effects of vitamin D demonstrated its protective role.

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Conclusion & Impact:Conclusion: Vitamin D treatment does not seem to have protective role against renal toxicity in sepsis.Nutrition with vitamin D in sepsis may have suppressive effect on antioxidant enzymes sush as CAT and GST due to reduced substrat level which use hydrogen peroxide (H2 O2 ) and GSH as a substrate

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Objective: Sepsis is a common cause of morbidity and mortality in the intensive care unit. Lipopolysaccharide (LPS)-induced excessive immune response is associated with multi-organ damage in sepsis. Excessive immune response causes multi-organ damage by increasing oxidative stress. Studies on the antioxidant effects of vitamin D demonstrated its protective role. In this study we aimed to investigate the effects of vitamin D on free radical metabolism in LPS injected rats.Methods: Twenty four wistar albino rats were separated into control, sepsis, sepsis+vitamin D and vitamin D groups. Sepsis was induced with single injection of LPS Esherichia coli (O111-b4) 16 mg/kg. Vitamin D was given 2 mg/kg 25 (OH) single dose via gavage for 3 days. Renal function tests were analyzed in serum. Tissue catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities were analyzed, and rat renal tissues were evaluated histopathologically.Results: SOD and GSH-Px activities were not significantly different between the groups. CAT activities were significantly decreased in all groups compared to control, this suppression was seen in the sepsis+vitamin D group versus sepsis group. GST activities were significantly decreased in sepsis and sepsis+vitamin D group compared to control, but GST activities were significantly elevated vitamin D group compare to sepsis and sepsis+vitamin D group. Blood urea nitrogen (BUN) and creatinine levels were significantly elevated in sepsis and sepsis+vitamin D group. Inflammation, expansion in bowman capsule were detected in sepsis and sepsis+vitamin D groups. Conclusion: Vitamin D treatment does not seem to have protective role against renal toxicity in sepsis.Nutrition with vitamin D in sepsis may have suppressive effect on antioxidant enzymes sush as CAT and GST due to reduced substrat level which use hydrogen peroxide (H2 O2 ) and GSH as a substrate

Özet

Amaç: Sepsis, yoğun bakımlarda mortalitenin ve morbiditenin en sık nedenidir. Sepsiste lipopolisakkaridin (LPS) neden olduğu aşırı immün yanıt oksidatif stres oluşturarak organ yetmezliğine neden olmaktadır. Vitamin D'nin antioksidan etkileriyle ilgili yapılan araştırmalarda vitamin D'nin koruyucu rolünün olduğu gösterilmiştir. Biz de çalışmamızda sepsiste serbest radikal metabolizması üzerine vitamin D'nin etkisini araştırmayı amaçladık.Metod: 24 adet dişi wistar albino rat kontrol grubu, sepsis grubu, vitamin D grubu, sepsis+vitamin D grubu olmak üzere 4 gruba bölündü. Sepsis 16 mg/kg dozunda LPS Escherichia coli (O111.b4) intraperitoneal yolla uygulanarak oluşturuldu. D vitamini 3 gün boyunca 2 mg/kg 25 (OH) Vitamin D3 (ayçiçek yağında çözülerek) gavaj yolula verildi. Böbrek fonksiyon testleri rat serumlarından çalışıldı.Doku katalaz (CAT), superoksit dismutaz (SOD), glutatyon peroksidaz(GSH-Px), glutatyon-S transferaz (GST) enzimleri ölçüldü. Dokuların histopatolojik incelemesi yapıldı.Bulgular: SOD ve GSH-Px aktivitelerinde gruplar arasında fark görülmedi. CAT aktivitelerinde en fazla sepsis+vitamin D grubunda olmak üzere tüm gruplarda kontrol grubuna göre anlamlı baskılanma görüldü. GST aktivitelerinin sepsis ve sepsis+vitamin D gruplarında baskılandığı görüldü. Bununla birlikte GST aktivitesinin vitamin D grubunda sepsis ve sepsis+vitamin D grubuna göre yüksek olduğu görüldü. Kan üre nitrojeni (BUN) ve kreatinin düzeylerinde sepsis ve sepsis+vitamin D gruplarında artış olduğu gözlendi. Sepsis ve sepsis+vitamin D gruplarında bowman kapsülünde genişleme ve inflamasyon görüldü.Sonuç: Sonuç olarak sepsiste vitamin D kullanılmasının renal hücreleri toksik etkiden koruyucu bir etkisinin olmadığını, buna karşılık özellikle sepsis durumunda kullanıldığında hidrojen peroksit (H2O2) ve GSH'yı substrat olarak kullanan anti oksidan enzimler (CAT, GST gibi) üzerine baskılayıcı etkisinin olduğunu gördük

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Cite This Article

KAVUTÇU, M., ATİKELER, G., ÇIRACI, M. Z., KOCABIYIK, M., CANBOLAT, O., ÇEVİK, C. (2022). Septik ratların böbrek dokularında serbest radikal metabolizmasına vitamin D'nin etkisi. Türk Biyokimya Dergisi, Cilt: 41 - Sayı: 5, 316–321. https://ajindex.com/articles/621043

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