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Journals / Medeniyet Medical Journal / 2019 / Cilt: 34 - Sayı: 1

The Protective Effect of Stobadine on Lipid Peroxidation and Paraoxonase-1 Enzyme Activity in the Liver Tissues of StreptozotocinInduced Diabetic Rats

Streptozotosin ile Diyabet Oluşturulan Rat Karaciğer Dokularında Lipit Peroksidasyonu ve Paraoksonaz-1 Enzim Aktivitesi Üzerine Stobadinin Koruyucu Etkisi

Mustafa KAVUTÇU
Nilhan Nurlu AYAN
Çimen KARASU
Pages
76–82
Publication Date
2022-10-19
DOI
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⚡ TL;DR(Too Long; Didn't Read)

In homogenized rat liver tissue samples after portioning by manual spectrophotometric method.Results: Malondialdehyde levels were significantly increased and paraoxonase-1 activities were significantly decreased in Group D compared to Groups C and STB (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Hyperglycemia is known to cause lipid peroxidation due to an increase in free radicals due to glucose autooxidation and by suppressing the antioxidant defense system of these radicals.
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Key Findings & Data:In this study, we aimed to investigate the effects of stobadineon lipid peroxidation and paraoxonase-1 enzyme activities in liver tissues of streptozotocin-induced diabetic rats.Methods: A total of 60 Wistar male rats, each weighing 250 g were randomly distributed into four groups; Control (C),Stobadine (STB), diabetic (D), STB treated diabetes (D+STB).
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Conclusion & Impact:In homogenized rat liver tissue samples after portioning by manual spectrophotometric method.Results: Malondialdehyde levels were significantly increased and paraoxonase-1 activities were significantly decreased in Group D compared to Groups C and STB (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Hyperglycemia is known to cause lipid peroxidation due to an increase in free radicals due to glucose autooxidation and by suppressing the antioxidant defense system of these radicals. Antioxidant therapy to managediabetes mellitus and its complications is an emerging trend. Stobadine is a pyridoindole compound and is knownto be an effective antioxidant in biological systems. In this study, we aimed to investigate the effects of stobadineon lipid peroxidation and paraoxonase-1 enzyme activities in liver tissues of streptozotocin-induced diabetic rats.Methods: A total of 60 Wistar male rats, each weighing 250 g were randomly distributed into four groups; Control (C),Stobadine (STB), diabetic (D), STB treated diabetes (D+STB). Diabetes was induced by a single intraperitoneal injectionof streptozotocin (55 mg/kg) to animals fasted overnight. Stobadine was administrated to rats as 25 mg/kg/day orallyfor four months. Rats were sacrificed after anesthesia; Malondialdehyde levels and PON-1 enzyme activities weremeasured. In homogenized rat liver tissue samples after portioning by manual spectrophotometric method.Results: Malondialdehyde levels were significantly increased and paraoxonase-1 activities were significantly decreased in Group D compared to Groups C and STB (p

Özet

Amaç: Hipergliseminin, glukoz oto-oksidasyonu sonucu serbest radikallerde bir artışa ve bu radikallerin antioksidan savunma sistemini baskılaması sonucu lipit peroksidasyonuna neden olduğu bilinmektedir. Diyabetes mellitus ve komplikasyonlarının önlenmesi ve tedavisinde antioksidan ajanların kullanımı gelişmekte olan bir trenddir. Stobadin pridoindol yapıda bir bileşiktir ve biyolojik sistemlerde etkili bir antioksidan olduğu bilinmektedir. Bu çalış- mada, streptozotosin ile diyabet oluşturulmuş sıçanların karaciğer dokularında, stobadinin lipid peroksidasyonu ve Paraoksonaz-1 enzim aktiviteleri üzerindeki etkilerini araştırmayı amaçladık. Yöntem: Her biri 250 g ağırlığındaki toplam 60 adet Wistar erkek sıçan rastgele; Kontrol (K), Stobadin (STB), Diyabet (D) ve STB ile tedavi edilmiş Diyabet (D+STB) gruplarını oluşturmak üzere dört eşit gruba ayrıldı. Diyabet; periton içi streptozotosin (55 mg/kg) enjeksiyonu ile, Stobadin tedavisi ise 4 ay süre ile 25 mg/kg/gün oral Stobadin verilerek yapıldı. Sıçanlar anestezi sonrası sakrifiye edildi. Porsiyonlandıktan sonra homojenize edilen sıçan karaciğer dokularında, Malondialdehid düzeyleri ve Paraoksonaz-1 enzim aktiviteleri manuel spektrofotometrik yöntemle ölçüldü. Bulgular: K ve STB grubu ile karşılaştırıldığında, D grubunda, Malondialdehid düzeyleri anlamlı artmış, Paraoksonaz-1 aktiviteleri anlamlı azalmış bulundu (p

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Cite This Article

KAVUTÇU, M., AYAN, N. N., KARASU, Ç. (2022). The Protective Effect of Stobadine on Lipid Peroxidation and Paraoxonase-1 Enzyme Activity in the Liver Tissues of StreptozotocinInduced Diabetic Rats. Medeniyet Medical Journal, Cilt: 34 - Sayı: 1, 76–82. https://ajindex.com/articles/579254

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