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Journals / Türk Biyokimya Dergisi / 2019 / Cilt: 44 - Sayı: 3

Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas

Yeşil, beyaz ve siyah çayın antioksidan, sitotoksik, tirozinaz inhibitör aktiviteleri ve fenolik profillerinin araştırılması

Ezgi ÖZTAŞ
Nuriye KORKMAZ
Ufuk ÖZGEN
Şeyda AKKAYA
Rezzan ALİYAZICIOĞLU
Mahmoud ABUDAYYAK
Merve BADEM
Sıla Özlem ŞENER
Pages
278–288
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

The methanol extract of green tea had thehighest cytotoxic potential, while the water extracts wereless toxic.Conclusion: The green, white, and black teas can beregarded as an important in terms of rich antioxidant,tyrosinase inhibitor, and cytotoxic activities in both thepharmaceutical and food industries.

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Objective & Hypothesis:tyrosinase inhibitory (TI) activities, and phenolic profileswith a new high performance liquid chromatography(HPLC) method of green, white and black teas.Methods: Antioxidant activities of the teas were examinedby means of scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidantpower (FRAP), and cupric reducing antioxidant capacity(CUPRAC).
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Key Findings & Data:The phenolic contents were investigated bymeans of HPLC. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to evaluatethe cytotoxic potential of tea extracts in cancer and normalcell lines.
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Conclusion & Impact:The methanol extract of green tea had thehighest cytotoxic potential, while the water extracts wereless toxic.Conclusion: The green, white, and black teas can beregarded as an important in terms of rich antioxidant,tyrosinase inhibitor, and cytotoxic activities in both thepharmaceutical and food industries.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

tyrosinase inhibitory (TI) activities, and phenolic profileswith a new high performance liquid chromatography(HPLC) method of green, white and black teas.Methods: Antioxidant activities of the teas were examinedby means of scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidantpower (FRAP), and cupric reducing antioxidant capacity(CUPRAC). The phenolic contents were investigated bymeans of HPLC. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to evaluatethe cytotoxic potential of tea extracts in cancer and normalcell lines. TI activity was investigated against diphenolase(DOPA), using kojic acid as a positive control.Results: The green tea extract exhibited stronger antioxidantthan white and black tea extracts. The greentea contained syringaldehyde, p-coumaric acid, benzoicacid, and quercetin. The white tea extract had thehighest tyrosinase inhibitor activity. The extracts exhibitedhigher cytotoxic potential toward cancer cells thannormal cells. The methanol extract of green tea had thehighest cytotoxic potential, while the water extracts wereless toxic.Conclusion: The green, white, and black teas can beregarded as an important in terms of rich antioxidant,tyrosinase inhibitor, and cytotoxic activities in both thepharmaceutical and food industries.

Özet

Amaç: Yeşil, beyaz ve siyah çayın antioksidan, sitotoksik ve tirozinaz inhibitör (TI) aktivitelerini ve yeni yüksek performanslı sıvı kromatografisi (HPLC) metodu ile fenolik içeriklerini belirlemek. Metod: Çayların antioksidan aktiviteleri; 2,2-difenil-1-pikril hidrazil (DPPH) serbest radikalin süpürülmesi, demir indirgeyici antioksidan güç (FRAP) ve bakır indirgeyici antioksidan kapasite (CUPRAC) ile incelendi. Fenolik içeriği HPLC ile incelendi. MTT yöntemi normal ve kanser hücre hatlarında çay ekstrelerinin sitotoksik potansiyelini değerlendirmek için kullanıldı. TI aktivite, pozitif kontrol olarak kojik asit kullanılarak difenolaza (DOPA) karşı incelendi.Bulgular: Yeşil çay ekstresi beyaz ve siyah çay ekstresinden daha güçlü antioksidan aktivite gösterdi. Yeşil çay şiringaldehid, p-kumarik asit, benzoik asit ve kersetin içerdi. Beyaz çay ekstresi en yüksek tirozinaz inhibitör aktivite gösterdi. Ekstreler, kanser hücrelerine karşı normal hücrelerden daha yüksek sitotoksik güce sahipti. Yeşil çayın su ekstresi daha az toksik iken, metanol ekstresi en yüksek sitotoksik güce sahipti. Sonuç: Yeşil, beyaz ve siyah çay hem farmasötik hem de besin endüstrisinde zengin antioksidan, tirozinaz inhibitör ve sitotoksik aktiviteleri açısından önemli olarak düşünülebilir.

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Cite This Article

ÖZTAŞ, E., KORKMAZ, N., ÖZGEN, U., AKKAYA, Ş., ALİYAZICIOĞLU, R., ABUDAYYAK, M., BADEM, M., ŞENER, S. Ö. (2022). Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. Türk Biyokimya Dergisi, Cilt: 44 - Sayı: 3, 278–288. https://ajindex.com/articles/620833

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