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Journals / Turkish Journal of Biology / 2000 / Cilt: 24 - Sayı: 3

Identification of lactoce plasmids in Lactococcus lactis subsp. lactis stains

Lactococcus lactis subsp. lactis suşlarında laktoz plazmidlerinin tanımlanması

Çağla TÜKEL
Mustafa AKÇELİK
Pages
405–424
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The molecular sizes of these lactose plasmids ranged between 26.3 and 38.5 kb.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Plasmids encoding lactic acid fermentation ability were investigated in 50 strains of L. lactis subsp. lactis.
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Key Findings & Data:Acriflavine was used for the elimination of lactose fermentation ability (Lac + ) from wild type strains.
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Conclusion & Impact:The molecular sizes of these lactose plasmids ranged between 26.3 and 38.5 kb.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Plasmids encoding lactic acid fermentation ability were investigated in 50 strains of L. lactis subsp. lactis. Acriflavine was used for the elimination of lactose fermentation ability (Lac + ) from wild type strains. Lactose negative (Lac - ) mutants were selected on lactose indicator agar media. Six different lactose plasmids were identified for fifty strains by comparison of plasmid profiles of Lac - and Lac + mutants. The molecular sizes of these lactose plasmids ranged between 26.3 and 38.5 kb.

Özet

Özet: Elli L. lactis subsp. lactis suşunda laktik asit fermentasyonu özelliğini kodlayan plazmidler araştırıldı. Doğal suşlardan laktik asit fermentasyonu özelliğinin (Lac+) eliminasyonu, akriflavin uygulaması ile sağlandı. Laktik asit fermentasyonu özelliğini kaybeden (Lac–) mutantlar, laktoz indikatör agar ortamlarında seçildi. Lac+ mutantlar ise plazmid analizleri yapılarak tanımlandı. Lac- ve Lac+ mutantların plazmid içeriklerinin karşılaştırılması sonucu, 50 suş için 6 farklı laktoz plazmidi saptandı. Bu laktoz plazmidlerinin moleküler büyüklükleri 26,3-38,5 kb arasında değişim gösterdi.

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Cite This Article

TÜKEL, Ç., AKÇELİK, M. (2022). Identification of lactoce plasmids in Lactococcus lactis subsp. lactis stains. Turkish Journal of Biology, Cilt: 24 - Sayı: 3, 405–424. https://ajindex.com/articles/600618

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References (15)

  1. Maeda, S.,Gasson, M. Cloning Expression And Location of the Streptococcus lactis Gene for Phospho-β- Galactosidase. J. General. Microbiol. 132; 331-340, 1986
  2. De Vos, W.M., Simmons, G. Molecular Cloning of Lactose Genes In Dairy Lactic Streptococci: The Phospho-β- Galactosidase Genes And Their Expression Products. Biochemie, 70; 461-473, 1988
  3. Venema, G. Molecular Biology And Genetic Modification of Lactococci. J. Dairy Sci. 76; 2133-2144, 1993
  4. Kok, J. Inducible Gene Expression And Environmentally Regulated Genes In Lactic Acid Bacteria. Antonie van Leeuwen. 70; 129-145, 1996
  5. Liu, C.Q., Dunn, N.W., Duan, K. Cloning And Sequence Analysis of a Plasmid Replicon From Lactococcus lactis subsp. cremoris FG2. J. Gen. Microbiol. 43; 75-80, 1997
  6. Akçelik, M. The Conjugal Plasmid pll10236 Encodes Lactose Fermentation Ability, Restriction Modification Activity And Bacteriocin Production And Immunity In Lactococcus lactis subsp. lactis LL102. Food Microbiology 16; 487- 494, 1999
  7. Mayer, H., Deixler, W.E., Bertsch, W. Plasmid Profiles of Mesophilic Lactic Acid Bacteria. Milchwirtschaftliche Ber. 114; 3-12, 1993
  8. Morita, H., Kamizono, K., Nakamura, S., Fujıta, Y., Sakata, R., Nagata, Y. A, McKay, L.L. Cloning of Citrate Permease Gene of Lactococcus lactis subsp. lactis biovar. diacetylactis N-7 And Expression In Citrate-Negative Lactococci. Milchwissenschaft, 52; 138-141, 1997
  9. Platteuw, C., Hugenholtz, J., Starrenburg, M., Alen-Boerritger, I., De Vos, W.M. Metabolic Engineering Of Lactococcus lactis: Influence of The Overproduction of α-Acetolactate Synthase In Strains Deficient In Lactate Dehydrogenase as a Function Of Culture Conditions. Appl. Environ. Microbiol. 61;3967-3971, 1995
  10. Harrigan, F.W., McCance, E.M. Laboratory Methods In Microbiology. Academic Press London, New York, 285s, 1966
  11. Huggins, R.A. Progress In Dairy Starter Culture Technology. Food Technology, 38; 41-50, 1984
  12. Holt, G.H., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T. Bergey’ s Manual of Determinative Bacteriology. Williams and Wilkins Co., Ninth Edition, 787 p., 1994
  13. McKay, L.L., Baldwin, K.A., Zottola, E.A. Loss of Lactose Metabolism In Lactic Streptococci. Appl. Microbiol. 23(6); 1090-1096, 1972
  14. Terzaghi, B.E., Sandine, W.E. Improved Medium For Lactic Streptococci And Their Bacteriophages. Appl. Microbiol. 29(6); 807-813, 1975
  15. Anderson, D.G., McKay, L.L. A Simple And Rapid Method For Isolating Large Plasmid DNA From Lactic S