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Journals / Turkish Journal of Veterinary and Animal Sciences / 2004 / Cilt: 28 - Sayı: 3

Conjugal Transfer and Stability of the Plasmids Determining Exopolysaccharide Production in Lactococcus lactis Strains

Ela KOÇER
Çağla TÜKEL
Pages
481–487
Publication Date
2004-03-01
DOI
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⚡ TL;DR(Too Long; Didn't Read)

On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 x 10-3 - 4.5 x 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.

📌 3 Key Takeaways

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Objective & Hypothesis:A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies.
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Key Findings & Data:Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 x 10-2 - 2.8 x 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40.
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Conclusion & Impact:On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 x 10-3 - 4.5 x 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Exopolysaccharide · conjugation · plasmid

Abstract

A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 x 10-2 - 2.8 x 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 x 10-3 - 4.5 x 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.

Özet

A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 x 10-2 - 2.8 x 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 x 10-3 - 4.5 x 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.

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Cite This Article

KOÇER, E., TÜKEL, Ç. (2004). Conjugal Transfer and Stability of the Plasmids Determining Exopolysaccharide Production in Lactococcus lactis Strains. Turkish Journal of Veterinary and Animal Sciences, Cilt: 28 - Sayı: 3, 481–487. https://ajindex.com/articles/177959

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