SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 4

Protein Profile and Plasmid Content of Lactococcus lactis subsp. lactis LL52 and Lactococcus lactis subsp. cremoris LC79 Strains under Several Stress Conditions

Rahmi LALE
Çağla TÜKEL
Mustafa AKÇELİK
Pages
247–252
Publication Date
2007-04-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Plasmid analysis indicated that there was no relationship between stress responses and plasmids in these bacteria.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Differences in the protein and plasmid content of 2 Lactococcus lactis strains, L. lactis subsp. lactis LL52 and L. lactis subsp. cremoris LC79, under the stresses of high and low temperature, osmotic shock, and low pH were determined.
📊
Key Findings & Data:We identified 3 new proteins with molecular masses of 16.0, 29.4, and 45.0 kDa as high temperature stress response specific in strain LL52.
💡
Conclusion & Impact:Plasmid analysis indicated that there was no relationship between stress responses and plasmids in these bacteria.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Lactococcus lactis · stress · protein

Abstract

Differences in the protein and plasmid content of 2 Lactococcus lactis strains, L. lactis subsp. lactis LL52 and L. lactis subsp. cremoris LC79, under the stresses of high and low temperature, osmotic shock, and low pH were determined. We identified 3 new proteins with molecular masses of 16.0, 29.4, and 45.0 kDa as high temperature stress response specific in strain LL52. High temperature stress did not cause any changes in the protein content of strain LC79. Proteins that were specific for salt stress and low pH stress responses ranged between 16.0 and 40.5 kDa, and 24.8 and 107.5 kDa, respectively, in both strains. No proteins were related to low temperature stress in LL52 and LC79 strains. Plasmid analysis indicated that there was no relationship between stress responses and plasmids in these bacteria.

Özet

Differences in the protein and plasmid content of 2 Lactococcus lactis strains, L. lactis subsp. lactis LL52 and L. lactis subsp. cremoris LC79, under the stresses of high and low temperature, osmotic shock, and low pH were determined. We identified 3 new proteins with molecular masses of 16.0, 29.4, and 45.0 kDa as high temperature stress response specific in strain LL52. High temperature stress did not cause any changes in the protein content of strain LC79. Proteins that were specific for salt stress and low pH stress responses ranged between 16.0 and 40.5 kDa, and 24.8 and 107.5 kDa, respectively, in both strains. No proteins were related to low temperature stress in LL52 and LC79 strains. Plasmid analysis indicated that there was no relationship between stress responses and plasmids in these bacteria.

🔖

Cite This Article

LALE, R., TÜKEL, Ç., AKÇELİK, M. (2007). Protein Profile and Plasmid Content of Lactococcus lactis subsp. lactis LL52 and Lactococcus lactis subsp. cremoris LC79 Strains under Several Stress Conditions. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 4, 247–252. https://ajindex.com/articles/122191

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Kim, W.S., Ren, J., Dunn, N.W.: Differentiation of Lactococcus lactis subspecies lactis and subspecies cremoris strains by their adaptive response to stresses. FEMS Microbiol. Lett., 1999; 171: 57-65. 2. Duwat, P., Ehrlich, S.D., Gruss, A.: The recA gene of Lactococcus lactis: characterization and involvement in oxidative and thermal Kim, W.S., Park, J.H., Tandianus, J.E., Ren, J., Su, P., Dunn, N.W.: A distinct physiological state of Lactococcus lactis cell that confers survival against a direct and prolonged exposure to severe stresses. FEMS Microbiol. Lett., 2002; 212: 203-208.
  2. Wood, J.M., Bremer, E., Csonka, L.N., Kraemer, R., Poolman, B., Van Der Heide, T., Smith, L.T.: Osmosensing and osmoregulatory compatible solute accumulation by bacteria. Comp. Biochem. Physiol. Part A., 2001; 130: 437-460.
  3. Terzaghi, B.E., Sandine, W.E.: Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol., 1975; 29: 807-813.
  4. Boutibonnes, P., Bisson, W.E., Thammavongs, B., Hartke, A., Panoff, J.M., Benachour, A., Auffray, Y.: Induction of thermotolerance by chemical agents in Lactococcus lactis subsp. lactis IL1403. Int. J. Food Microbiol., 1995; 25: 83-94.
  5. Heukeshoven, J., Dernick, R.: Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix. Electrophoresis, 1995; 16: 748-756.
  6. Anderson, D.G., McKay, L.L.: A simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol., 1983; 46: 549-552.
  7. Stougaard, P., Molin, S.: Vertical dye-buoyant density gradients for rapid analysis and preparation of plasmid DNA. Anal. Biochem., 1981; 118: 191-193.
  8. Kim, W.S., Park, J.H., Ren, J., Su, P., Dunn, N.W.: Survival response and rearrangement of plasmid DNA of Lactococcus lactis during long-term starvation. Appl. and Environ. Microbiol., 2001; 67: 4594-4602.
  9. Weber, A., Jung, K.: Profiling early osmostress-dependent gene expression in Escherichia coli using DNA macroarrays. J. Bacteriol., 2002; 184: 5502-5507.
  10. Mogk, A., Homuth, G., Scholz, C., Kim, L., Schmid, F.X., Schumann, W.: The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis. EMBO J., 1997; 16: 4579-4590.
  11. Koch, B., Kilstrup, M., Vogensen, F.K., Hammer, K.: Induced levels of heat shock proteins in dnaK mutant of Lactococcus lactis. J. Bacteriol., 1998; 180: 3873-3881.
  12. Guillot, A., Obis, D., Mistou, M.Y.: Fatty acid membrane composition and activation of glycine-betaine transport in Lactococcus lact