The results of thesemethods confirmed the findings of absorption spectra study that the compound interactswith the DNA
📌 3 Key Takeaways
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Objective & Hypothesis:The DNA binding activity of previously synthesized and characterized 2(3), 9(10), 16(17), 23(24) –Tetrakis 4-(4-(2-phenylprop-2-yl) phenoxy) phthalocyanine compound (PcF) with CT-DNA was studied using UV/Vis, emission spectroscopic titrations,the melting temperature, viscosity measurement, and agarose gel electrophoresis methodsin a Tris-HCl buffer solution at a pH of 7.1 at room temperature.
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Key Findings & Data:The absorption titrationspectra studies of PcF showed that absorbance intensities were decreased with increasing ofconcentrations of CT-DNA and the finding proved that the compound interacts with theDNA.
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Conclusion & Impact:The results of thesemethods confirmed the findings of absorption spectra study that the compound interactswith the DNA
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
The DNA binding activity of previously synthesized and characterized 2(3), 9(10), 16(17), 23(24) –Tetrakis 4-(4-(2-phenylprop-2-yl) phenoxy) phthalocyanine compound (PcF) with CT-DNA was studied using UV/Vis, emission spectroscopic titrations,the melting temperature, viscosity measurement, and agarose gel electrophoresis methodsin a Tris-HCl buffer solution at a pH of 7.1 at room temperature. The absorption titrationspectra studies of PcF showed that absorbance intensities were decreased with increasing ofconcentrations of CT-DNA and the finding proved that the compound interacts with theDNA. Addition to absorption titration study, emission spectroscopic titration, the meltingtemperature, viscosity measurement, and agarose gel electrophoresis methods were alsoperformed to investigate the binding activities of PcF with the DNA. The results of thesemethods confirmed the findings of absorption spectra study that the compound interactswith the DNA
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Cite This Article
AĞIRTAŞ, M. S., ARSLANTAŞ, A., DURMUŞ, C. (2022). An Investigation upon DNA Interaction of 2 (3), 9 (10), 16 (17), 23 (24) –Tetrakis 4-(4-(2-phenylprop-2-yl) phenoxy) Phthalocyanine Compound. Hittite Journal of Science and Engineering, Cilt: 7 - Sayı: 2, 81–87. https://ajindex.com/articles/537948
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