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AI Summary & Key Takeaways
AI Insight⚡ TL;DR(Too Long; Didn't Read)
It was concluded that 0.75% ONPG concentration is suitable for high beta-galactosidase activity.
📌 3 Key Takeaways
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Objective & Hypothesis:In this study, optimum conditions affecting the activity of beta-galactosidase enzyme from Alternaria alternata were determined and its stability was investigated.
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Key Findings & Data:It was concluded that 0.75% ONPG concentration is suitable for high beta-galactosidase activity.
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Conclusion & Impact:The enzyme was stable between 20-50$^circ$ C in the pH range 3.0-7.0 and kept more than 50% of its original activity after 40 min at 60$^circ$ C.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
In this study, optimum conditions affecting the activity of beta-galactosidase enzyme from Alternaria alternata were determined and its stability was investigated. It was concluded that 0.75% ONPG concentration is suitable for high beta-galactosidase activity. The optimum temperature and pH were found to be 50$^circ$ C and 5, respectively. The enzyme was stable between 20-50$^circ$ C in the pH range 3.0-7.0 and kept more than 50% of its original activity after 40 min at 60$^circ$ C.
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Cite This Article
SEYİS, I. (2022). Characterization of beta-glactosidase from Alternaria alternata. Hacettepe Journal of Biology and Chemistry, Cilt: 32 - Sayı:, 105–110. https://ajindex.com/articles/529425