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Journals / Türk Biyokimya Dergisi / 2015 / Cilt: 40 - Sayı: 6

Beş farklı klinik Klebsiella türünün antibiyotik dirençliliği ile plazmid profillerinin karşılaştırmalı olarak değerlendirilmesi

Comparative assessment of five clinical Klebsiella isolates in terms of antibiotic resistance and plasmid profiles

Işıl SEYİS BİLKAY
Nermin Hande AVCIOĞLU
Pages
448–455
Publication Date
2022-11-03
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Among these profiles, P1 (52.23%) was the most observed type which exhibits >10 kbp plasmid dNA band and this profile was isolated from all strains Conclusion: Finally, it is observed that antibiotic resistancecan be due to the plasmid or chromosomal sourcesand different strains of the same genus may exhibit thesame plasmid profile because of the plasmid transformationfrom one strain to another.

📌 3 Key Takeaways

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Objective & Hypothesis:objective: This study is firstly aimed to biotype and to investigate the antibiotic resistance profiles of Klebsiella strains which were isolated from clinical materials.
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Key Findings & Data:Also plasmid profile types of Klebsiella species were investigated and the results were compared with each other.Methods: Klebsiella strains were biotyped by BBL Enterotube ii and the antibiotic resistance was tested by Kirby Bauer disc diffusion method. in order to analyse Klebsiella strains genotypically, plasmid dNA’s of them were isolated and generated plasmid profile types determined by dice coefficients of similarity.Results: According to biotyping, Klebsiella strains were distributed to five different species (K. pneumoniae, K. ornithinolytica, K. oxytoca, K. terrigena, K. rhinoscleromatis).
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Conclusion & Impact:Among these profiles, P1 (52.23%) was the most observed type which exhibits >10 kbp plasmid dNA band and this profile was isolated from all strains Conclusion: Finally, it is observed that antibiotic resistancecan be due to the plasmid or chromosomal sourcesand different strains of the same genus may exhibit thesame plasmid profile because of the plasmid transformationfrom one strain to another.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

objective: This study is firstly aimed to biotype and to investigate the antibiotic resistance profiles of Klebsiella strains which were isolated from clinical materials. Also plasmid profile types of Klebsiella species were investigated and the results were compared with each other.Methods: Klebsiella strains were biotyped by BBL Enterotube ii and the antibiotic resistance was tested by Kirby Bauer disc diffusion method. in order to analyse Klebsiella strains genotypically, plasmid dNA’s of them were isolated and generated plasmid profile types determined by dice coefficients of similarity.Results: According to biotyping, Klebsiella strains were distributed to five different species (K. pneumoniae, K. ornithinolytica, K. oxytoca, K. terrigena, K. rhinoscleromatis). As well as K. pneumoniae (37.33%) was the most isolated strain, K. terrigena (8.95%) which is not found to be investigated as much as other strains in the literature, was also observed. Additionally, it is determined that Klebsiella strains were resistant to at least one and at most seven antibiotics. With a similarity coefficient of 84%, it was observed that five different Klebsiella species displayed 17 different plasmid profile types. Among these profiles, P1 (52.23%) was the most observed type which exhibits >10 kbp plasmid dNA band and this profile was isolated from all strains Conclusion: Finally, it is observed that antibiotic resistancecan be due to the plasmid or chromosomal sourcesand different strains of the same genus may exhibit thesame plasmid profile because of the plasmid transformationfrom one strain to another.

Özet

Amaç: Bu çalışma öncelikle klinik materyallerden izole edilen Klebsiella türlerinin biyotiplendirilmesi ve antibiyotik direnç profillerinin belirlenmesini amaçlamaktadır. Bunun yanı sıra çalışmamızda Klebsiella türlerinin plazmid profilleri incelenmiş ve antibiyotik direnç profilleri ile karşılaştırılarak yorumlanmıştır.Metod: Klebsiella türleri BBL Enterotube ii kullanılarak biyotiplemesi yapıldı ve antibiyotik dirençlilikleri Kirby Bauer disk difüzyon metodu kullanılarak test edildi. Klebsiella suşlarının genotipik olarak analiz edilmesi için söz konusu türlerin plazmid dNA'ları izole edildi ve oluşturulan plazmid tipleri benzerlik katsayısı kullanılarak belirlendi.Bulgular: Biyotipleme sonuçlarına göre Klebsiella türlerinin 5 farklı türe (K. pneumoniae, K. ornithinolytica, K. oxytoca, K. terrigena, K. rhinoscleromatis) ayrıldığı belirlendi. K. pneumoniae (%37.33) türünün en fazla izole edilen tür olduğu, aynı zamanda literatürde diğer Klebsiella türlerine kıyasla daha az çalışılmış olan K. terrigena'nın (%8.95) da çalışmamızda izole edildiği gözlendi. Ayrıca, Klebsiella türlerinin en az 1 en fazla 7 antibiyotiğe dirençli oldukları belirlendi. 5 farklı Klebsiella türünün %84 benzerlik katsayısı ile 17 farklı plazmid profil tipine ayrıldığı saptandı. Bu profiller içerisinde >10 kbç plasmid dNA bantı içeren P1 (%52.23) plazmid profil tipinin Klebsiella türlerinin hepsinde en fazla izole edilen tip olduğu belirbelirlendi.Sonuç: Sonuç olarak, antibiyotik direncinin plazmid veya kromozomal kaynaklı olabileceği ve aynı cins içerisinde yer alan farklı türlerin, bir türden diğer türe aktarılan plazmidler nedeniyle aynı plazmid profil tipini gösterebileceği saptandı

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Cite This Article

BİLKAY, I. S., AVCIOĞLU, N. H. (2022). Beş farklı klinik Klebsiella türünün antibiyotik dirençliliği ile plazmid profillerinin karşılaştırmalı olarak değerlendirilmesi. Türk Biyokimya Dergisi, Cilt: 40 - Sayı: 6, 448–455. https://ajindex.com/articles/621099

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