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Journals / Clinical Dentistry and Research / 2017 / Cilt: 41 - Sayı: 2

REAL-TIME POLYMERASE CHAIN REACTION QUANTIFICATION OF MICRO-LEAKAGE BETWEEN TITANIUM–IMPLANT AND ZIRCONIUM– IMPLANT INTERFACES

TİTANYUM-İMPLANT VE ZİRKOYA-İMPLANT ARAYÜZLERİNDEKİ MİKROSIZINTININ GERÇEK ZAMANLI PZR KULLANILARAK NİCEL ÖLÇÜMÜ

Cem SAHİN
Alper ERGİN
Pages
83–88
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The microorganism proliferations at implant-zirconiainterface were significantly higher than corresponding implanttitaniuminterface at each time intervals (p

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Objective & Hypothesis:Background and Aim: Zirconia superstructures areexpressed to have better specifications like aesthetics andbiocompatibility; however, the micro-leakage is still undefined.The aim of this study was to measure bacterial micro-leakagealong two different implant-abutment interfaces with RealtimePCR quantitatively at in-vitro conditions.Materials and Methods: Three microliters of FusobacteriumNucleatum standard density were inoculated into innercompartment of the implant’s fixtures using micropipette.Two different abutment types (zirconia=7, titanium=7) withinternal-hex geometries were fixed onto their own fixture.The combinations were immersed in 5 ml of thioglycolatemedia.
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Key Findings & Data:Media (0.2 ml) were taken from tubes for DNAextraction.
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Conclusion & Impact:The microorganism proliferations at implant-zirconiainterface were significantly higher than corresponding implanttitaniuminterface at each time intervals (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background and Aim: Zirconia superstructures areexpressed to have better specifications like aesthetics andbiocompatibility; however, the micro-leakage is still undefined.The aim of this study was to measure bacterial micro-leakagealong two different implant-abutment interfaces with RealtimePCR quantitatively at in-vitro conditions.Materials and Methods: Three microliters of FusobacteriumNucleatum standard density were inoculated into innercompartment of the implant’s fixtures using micropipette.Two different abutment types (zirconia=7, titanium=7) withinternal-hex geometries were fixed onto their own fixture.The combinations were immersed in 5 ml of thioglycolatemedia. Media (0.2 ml) were taken from tubes for DNAextraction. High-Pure PCR Template Preparation Protocolwith Proteinase K was used for the process. Real-time PCRanalysis was performed with Lightcycler 480 system usingF.nucleatum specific; F:CGCAGAAGGTGAAAGTCCTGTAT,R:TGGTCCTCACTGATTCACACAGA (101 bp) primer set. Mann-Whitney U test was used to compare 2nd, 4th and 8th day ofcorresponding Zirconia and Titanium groups.Results: The highest mean cq number (DNA quantification)was obtained at the day 8 of the zirconia group and followed bymean cq numbers obtained at the 4th and 2nd day of zirconiagroups. The microorganism proliferations at implant-zirconiainterface were significantly higher than corresponding implanttitaniuminterface at each time intervals (p

Özet

Amaç: Zirkonya üstyapılar estetik ve biyouyumluluk gibi üstün özellikleriyle tanımlanır, ancak mikrosızıntı halen tanımsızdır. Bu çalışmanın amacı gerçek zamanlı PZR kullanarak iki farklı implant-abutment birleşimindeki mikrosızıntıyı nicel olarak incelemektir. Gereç ve Yöntem: Mikropipet kullanarak 3 mikrolitre Fuzobakterium Nukleatum standardı implantın iç kompartımanına yerleştirildi. İnternal hex geometrisine sahip 2 farklı üstyapı (Zirkonya=7, Titanyum=7) implant üzerine sabitlendi. Kombinasyon 5 Ml tiyoglukolat içerisine daldırıldı. DNA çıkarımı için 0.2 ml sıvı örneği alındı. Bu süreçte protein K içerikli yüksek saflıktaki PCR hazırlık kiti kullanıldı. Analizler, F. Nucleatum primer spesifiği: F:CGCAGAAGGTGAAAGTCCTGTAT, R:TGGTCCTCACTGATTCACACAGA (101 bp) kullanılarak Lightcycler 480 cihazında yapıldı. Mann-Whitney U ve Friedman analizleri kullanılarak 2, 4 ve 8. günlerdeki Titanyum ve Zirkonya örnekler karşılaştırıldı. Bulgular: En yüksek Cq sayısı (DNA sayısı) Zirkonya grupta 8. gün sonunda gözlemlendi. Bunu 4. ve 2. günlerdeki Zirkonya gruplar takip etti. Tüm zaman aralıklarında implant-Zirkonya ara yüzündeki üreme İmplant-Titanyum ara yüzündekinden belirgin şekilde fazla oldu. (p

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Cite This Article

SAHİN, C., ERGİN, A. (2022). REAL-TIME POLYMERASE CHAIN REACTION QUANTIFICATION OF MICRO-LEAKAGE BETWEEN TITANIUM–IMPLANT AND ZIRCONIUM– IMPLANT INTERFACES. Clinical Dentistry and Research, Cilt: 41 - Sayı: 2, 83–88. https://ajindex.com/articles/474585

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