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Journals / Clinical Dentistry and Research / 2017 / Cilt: 41 - Sayı: 3

EFFECT OF NICOTINE ON PERIODONTAL LIGAMENT DERIVED CELLS CULTURED UNDER MECHANICAL STRESS

MEKANİK STRESS ALTINDA KÜLTÜRE EDİLEN PERİODONTAL LİGAMENT KAYNAKLI HÜCRELERDE NİKOTİNİN ETKİLERİ

Kenichi MATSUZAKA
Kenji SUEİSHİ
Taiki MORİKAWA
Yuki IİJİMA
Takashi INOUE
Pages
106–113
Publication Date
2022-10-11
DOI
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Expressionof IL-6 and VEGF mRNA in the NI+MS group, NI group and MSgroup were significantly higher than that of the CONT group.Conclusion: Nicotine suppressed expression of OPG andaccelerated osteoclastogenesis in PDL-derived cells undercentrifugal force.

📌 3 Key Takeaways

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Objective & Hypothesis:Background and Aim: The purpose of this study was toinvestigate the effects of nicotine on periodontal ligament(PDL) cells cultured under mechanical stress.Materials And Methods: Periodontal ligament-derived cellsobtained from rat incisors were cultured with nicotine (20ng/ml, NI), under centrifugal force (2000 rpm/20 min, MS) asexperimental group (NI+MS).
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Key Findings & Data:The mRNAlevels of RANKL, osteoprotegerin (OPG), interleukin-6 (IL-6),and vascular endothelial growth factor (VEGF) were analyzedusing quantitative real-time polymerase chain reaction at day1 of culture.Results: RANKL positive cells and RANKL mRNA expressionin the NI+MS group, NI group, and MS group were significantlyhigher than that of the CONT group.
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Conclusion & Impact:Expressionof IL-6 and VEGF mRNA in the NI+MS group, NI group and MSgroup were significantly higher than that of the CONT group.Conclusion: Nicotine suppressed expression of OPG andaccelerated osteoclastogenesis in PDL-derived cells undercentrifugal force.
🔬Design:Research Article
📍Scope:2000 Verileri
⚙️Variables:Domain Parameters

Abstract

Background and Aim: The purpose of this study was toinvestigate the effects of nicotine on periodontal ligament(PDL) cells cultured under mechanical stress.Materials And Methods: Periodontal ligament-derived cellsobtained from rat incisors were cultured with nicotine (20ng/ml, NI), under centrifugal force (2000 rpm/20 min, MS) asexperimental group (NI+MS). The control groups were treatedwith NI alone (NI), under MS without NI (MS), and withoutany treatment (CONT). An immunofluorescence study usingan anti-receptor activator of nuclear factor-kappa B ligand(RANKL) primary antibody was also performed. The mRNAlevels of RANKL, osteoprotegerin (OPG), interleukin-6 (IL-6),and vascular endothelial growth factor (VEGF) were analyzedusing quantitative real-time polymerase chain reaction at day1 of culture.Results: RANKL positive cells and RANKL mRNA expressionin the NI+MS group, NI group, and MS group were significantlyhigher than that of the CONT group. Expression of RANKLmRNA of the NI+MS group was higher than that of the CONTgroup and NI group. Expression of OPG mRNA in the MS groupwas significantly higher than that of other 3 groups. Expressionof IL-6 and VEGF mRNA in the NI+MS group, NI group and MSgroup were significantly higher than that of the CONT group.Conclusion: Nicotine suppressed expression of OPG andaccelerated osteoclastogenesis in PDL-derived cells undercentrifugal force.

Özet

Amaç: Bu çalışmanın amacı mekanik stress altında kültüre edilen periodontal ligament hücrelerinde (PDL) nikotinin etkilerini araştırmaktır. Gereç ve yöntemler: Fare kesici dişlerinden elde edilen periodontal kaynaklı hücreler, deney grubu olarak (NI+MS) nikotin ile (20 ng/ml, NI) santrifüjel kuvvet altında (2000 rpm/20 dk, MS) kültüre edilmişlerdir. Kontrol grupları, sadece NI (NI), MS altında NI içermeyen (MS), ve herhangi bir işlem uygulanmamış (CONT) olarak ayrılmıştır. Nüklear factor-kappa B ligand (RANKL) primer antikorunun antireseptör aktivatörü kullanılarak immünoflorasan bir çalışma yapılmıştır. RANKL, osteoprotegerin (OPG), interlökin-6 (IL-6), ve vasküler endotelyal büyüme faktörü (VEGF) mRNA seviyeleri kuantitatif real-time polimeraz zincir reaksiyonu kullanilarak 1 günlük kültürde analiz edilmiştir. Bulgular: RANKL pozitif hücreler ve NI+MS, NI ve MS gruplarındaki mRNA salınımı kontrol grubundan belirgin bir şekilde daha yüksek bulunmuştur. NI+MS grubundaki RANKL mRNA salınımı CONT ve NI gruplarından daha yüksek bulunmuştur. MS grubundaki OPG mRNA salınımı diğer üç gruptan belirgin bir şekilde yüksektir. NI+MS, NI ve MS gruplarındaki VEGF ve IL-6 mRNA salınımı CONT grubundan belirgin bir şekilde yüksektir. Sonuçlar: Nikotin, santrifüjel kuvvet altındaki PDL-kaynaklı hücrelerdeki OPG salınımını baskılamış ve osteoklastogenezisi hızlandırmıştır

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Cite This Article

MATSUZAKA, K., SUEİSHİ, K., MORİKAWA, T., IİJİMA, Y., INOUE, T. (2022). EFFECT OF NICOTINE ON PERIODONTAL LIGAMENT DERIVED CELLS CULTURED UNDER MECHANICAL STRESS. Clinical Dentistry and Research, Cilt: 41 - Sayı: 3, 106–113. https://ajindex.com/articles/474586

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