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Journals / Clinical Dentistry and Research / 2018 / Cilt: 42 - Sayı: 1

THE EFFECT OF EPITHELIAL RESTS OF MALASSEZ CELLS ON THE OSTEOGENIC DIFFERENTIATION OF MOUSE iPS CELLS

PLURİPOTENT KÖK HÜCRELERİN OSTEOJENİK FARKLILAŞMASI ÜZERİNE MALASSEZ EPİTEL ARTIKLARININ ETKİSİ

Koichi Naono
Tungalag Ser Od
Akram Al Wahabi
Kenichi Matsuzaka
Takashi Inoue
Pages
3–10
Publication Date
2022-10-11
DOI
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⚡ TL;DR(Too Long; Didn't Read)

RUNX2, BSP and OCN mRNA expression levels by miPS cells cultured on MMC-treated ERM cells were significantly higher than miPS cells cultured on FA-treated ERM cells at day 10 (p

📌 3 Key Takeaways

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Objective & Hypothesis:Background and Aim: The purpose of this study was to evaluate the epithelial rest of Malassez (ERM) cell on the differentiation of mouse induced pluripotent stem (miPS) cells to osteogenic cells.
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Key Findings & Data:The miPS cells on each kind of feeder cells were evaluated morphologically and their mRNA expressions levels of osteogenic markers Runt-related transcription factor 2 (RUNX2), secreted phosphoprotein1 used for bone sialoprotein (BSP) and bone gammacarboxyglutamate protein3 used for osteocalcin (OCN) after 6 and 10 days of culture.
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Conclusion & Impact:RUNX2, BSP and OCN mRNA expression levels by miPS cells cultured on MMC-treated ERM cells were significantly higher than miPS cells cultured on FA-treated ERM cells at day 10 (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background and Aim: The purpose of this study was to evaluate the epithelial rest of Malassez (ERM) cell on the differentiation of mouse induced pluripotent stem (miPS) cells to osteogenic cells. Materials and Methods: The porcine ERM cells treated with or without mitomycin C (MMC) or formaldehyde acid (FA) were used as feeder cells for miPS cells culture. The miPS cells on each kind of feeder cells were evaluated morphologically and their mRNA expressions levels of osteogenic markers Runt-related transcription factor 2 (RUNX2), secreted phosphoprotein1 used for bone sialoprotein (BSP) and bone gammacarboxyglutamate protein3 used for osteocalcin (OCN) after 6 and 10 days of culture. Results: The colonies of miPS cells cultured on MMC-treated ERM cells were flattened and spreading. The miPS cells cultured on FA-treated ERM cells were more accumulated than the others. RUNX2, BSP and OCN mRNA expression levels by miPS cells cultured on MMC-treated ERM cells were significantly higher than miPS cells cultured on FA-treated ERM cells at day 10 (p

Özet

Amaç: Bu çalışmanın amacı Malassez epithelial artıklarının (ERM) indüklenmiş fare pluripotent kök hücrelerinin (miPS) osteojenik hücrelere farklılaşması üzerine etkisini incelemektir. Gereç ve Yöntem: Mitomisin C (MMC) veya formaldehit asit (FA) ile muamele edilmiş veya edilmemiş domuz ERM hücreleri miPS hücre kültürleri için besleyici hücre olarak kullanıldı. Herbir besleyici hücre üzerindeki MiPS hücre kültürünün 6 ve 10 günündeki morfolojik değişimler ve osteojenik markerların; Runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP) için kullanılan phosphoprotein1 ve osteocalcin (OCN) için kullanılan gammacarboxyglutamate protein3’ün mRNA ekspresyon düzeyleri incelendi. Bulgular: MMC ile muamele edilen ERM hücreleri üzerinde çoğalan miPS hücre kolonileri yayılmış ve düzleşmişti. FA ile muamele edilen ERM hücreleri üzerine kültüre olan miPS hücreleri diğerlerine gore daha fazla çoğalmıştı. MMC ile muamele edilen ERM hücreleri üzerinde çoğalan miPS hücrenin RUNX2, BSP ve OCN mRNA ekspresyon düzeyleri 10 günlük kültürde FA ile muamele edilen ERM hücre ortamında çoğalan miPS hücrelerinden önemli derecede daha fazlaydı. Sonuç: Besleyici hücre olarak kullanılan MMC-ile muamele edilen ERM hücreleri miPS hücrelerinin osteojenik farklılaşmasını artırır.

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Cite This Article

Naono, K., Od, T. S., Wahabi, A. A., Matsuzaka, K., Inoue, T. (2022). THE EFFECT OF EPITHELIAL RESTS OF MALASSEZ CELLS ON THE OSTEOGENIC DIFFERENTIATION OF MOUSE iPS CELLS. Clinical Dentistry and Research, Cilt: 42 - Sayı: 1, 3–10. https://ajindex.com/articles/474573

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