SavedAdd your journal

Journals / Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi / 2017 / Cilt: 18 - Sayı: 1

Insecticidal activity of isolated bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)

Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)'den izole edilen bakterilerin insektisidal aktivitesi

Nurcan ALBAYRAK İSKENDER
Serkan ÖRTÜCÜ
Yaşar AKSU
Pages
56–62
Publication Date
2022-10-11
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The highest insecticidal activity was obtained from L. sphaericus (Abk1) with 30% after application (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The fall webworm (Hyphantria cunea) is a polyphagous pest with numerous host plants.
📊
Key Findings & Data:Some morphological, biochemical and other phenotypic characteristics (with API 20E, API 50 CH, API Staph and API Coryne kits) of bacterial isolates were determined.
💡
Conclusion & Impact:The highest insecticidal activity was obtained from L. sphaericus (Abk1) with 30% after application (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The fall webworm (Hyphantria cunea) is a polyphagous pest with numerous host plants. In the present study, the bacterial flora of H.cunea was investigated to identify new organisms that can be used as microbial control agent against the pest. Six bacteria were isolated and cultured from H. cunea. Some morphological, biochemical and other phenotypic characteristics (with API 20E, API 50 CH, API Staph and API Coryne kits) of bacterial isolates were determined. In addition, 16S rRNA gene region was sequenced. As a result of the studies conducted, bacterial isolates were identified as Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) and Microbacterium oxydans (Abk8). All bacterial isolates were tested for 12 days against third-fourth instar larvae of H. cunea. The highest insecticidal activity was obtained from L. sphaericus (Abk1) with 30% after application (p

Özet

Amerikan Beyaz Kelebeği, Hyphantria cunea (Drury) sayısız konukçu bitkisi olan polifag bir zararlıdır. Bu çalışmada, H. cunea'nın bakteriyal florası zararlıya karşı mikrobiyal kontrol ajanı olabilecek yeni organizmaları identifiye etmek için araştırıldı. H. cunea'dan altı bakteri izole edilerek saflaştırıldı. Bakteriyal izolatların morfolojik, biyokimyasal, diğer fenotipik özellikleri (API 20E, API 50 CH, API Staph ve API Coryne) belirlendi. İlave olarak, 16S rRNA gen sekans analizi de yapıldı. Yapılan çalışmalar sonucunda izolatlar; Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) ve Microbacterium oxydans (Abk8) olarak tanımlandı. Bütün bakteriler H. cunea'nın üçüncü-dördüncü gömlek larvalarına karşı oniki gün boyunca test edildi. En yüksek insektisidal aktivite %30 ile L. sphaericus (Abk1)'dan elde edildi (p

🔖

Cite This Article

İSKENDER, N. A., ÖRTÜCÜ, S., AKSU, Y. (2022). Insecticidal activity of isolated bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, Cilt: 18 - Sayı: 1, 56–62. https://ajindex.com/articles/450703

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Abbott WS (1925) A method of computing the effectiveness of aninsecticide. J Econ Entomol 18: 265-267.
  2. Albayrak Iskender N, Örtücü S, Aksu Y (2012). Pathogenicity of three isolates of the entomopathogenic fungi Beauveria bassiana to control Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) larvae. Kırgızistan-Türkiye Manas Üniversitesi, Fen Bilimleri Dergisi 2 (13), 16-21.
  3. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
  4. Ben-Dov E, Boussiba S, Zaritsky A (1995). Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis. J Bacteriol 10: 2581-2587.
  5. Chandel K, Mendki MJ, Parikh RH, Kulkarni G, Tikar SN, Sukumaran D, Prakash S, Parashar BD, Shouche YS, Veer V (2013) Midgut microbial community of Culex quinquefasciatus mosquito populations from India. Plos One 8: 11. Geetha I, Manonmani AM, Prabakaran G (2011) Bacillus amyloliquefaciens: A mosquitocidal bacterium from mangrove forests of Andaman and Nicobar islands, India. Acta Trop 120: 155- 159. Gupta AK, Nayduch D, Verma P, Shah B, Ghate HM, Patole MS, Yogesh YS (2012) Phylogenetic characterization of bacteria in the gut of house flies (Musca domestica L.). Fems 79: 581-593.
  6. Harley JP, Prescott LM (2002) Laboratory Exercises in Microbiology. Fifth Edition New York: The McGraw-Hill Compaines. Huang S, Sheng P, Zhang H (2012) Isolation and ıdentification of cellulolytic bacteria from the gut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae). Int J Mol Sci 13: 2563-2577.
  7. Leroy PD, Sabri A, Heuskin S, Thonart P, Lognay G, Verheggen FJ, Francis F, Brostaux Y, Felton GW, Haubruge E (2011) Microorganisms from aphid honeydew attract and enhance the efficacy of natural enemies. Nat Commun 2: 1-7.
  8. Moar WJ, Pusztzai-Carey M, Mack TP (1995) Toxity of purified proteins and the HD-1 strain from Bacillus thuringiensis againts lesser cornstalk borer (Lepidoptera: Pyralidae). J Econ Entomol 88: 606- 609. Mongodin EF, Shapir N, Daugherty SC, DeBoy RT, Emerson JB, Shvartzbeyn A, Radune D, Vamathevan J, Riggs F, Grinberg V, Khouri H, Wackett LP, Nelson KE, Sadowsky MJ (2006) Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1. PLoS Genet 2: 2094-2106.
  9. Ozsahin E, Sezen K, Demir I, Demirbag Z (2014) Bacterial isolates from Palomena prasina (Hemiptera: Pentatomidae) include potential microbial control agents. Biocontrol Sci Techn 24: 1039-1051. Suryadi BF,